Expi297


Precise protein expression is all in the fold

For structural biologists, insoluble and misfolded proteins mean starting over. The Expi293 Expression System is precisely designed with suspension 293 cells, optimized medium, and transfection reagent to enable native folding and maximum protein yields. Now your structural study can get the biological relevance it deserves.

Transient HEK 293 Expression Protocol

Learn how to produce recombinant proteins with the Gibco™ Expi293™ Expression System and achieve gram per liter protein yields in a transient HEK 293 system.

Expi293 Expression System for Structural Biology Applications

The Expi293 Expression System is precisely designed with suspension 293 cells, optimized medium, and transfection reagent to enable native folding and maximum protein yields. Now your structural study can get the biological relevance it deserves.

Rapid and ultrahigh-yield protein production in mammalian cells

The Expi293 Expression System is a rapid method of producing milligram to gram quantities of recombinant protein in mammalian cells (Figure 1).

Figure 1. Expression of human IgG and Fc-tagged Cripto achieve expression levels of over 1 g/L in the Expi293 ™ Expression System.

Study correctly folded, active protein and assembled complexes

The Expi293 Expression System is based on expression in mammalian 293 cells, which offers the benefits of native folding and posttranslational modifications. The two figures below show purification of the HDAC3/SMRT-DAD complex from 293 cells and E. coli. The 293 cells yield a stable pure HDAC3 complex (Figure 2A), whereas expression in E. coli (Figure 2B) does not.
 

Figure 2. Purification of the HDAC3/SMRT-DAD complex from (A) mammalian 293 cells and (B) E. coli. In both cases the protein complex was purified using an affinity tag on SMRT: Flag-tag for the mammalian-expressed proteins, and GST-tag for the E. coli-expressed proteins. The HDAC3 was untagged.

 

Fast and simple protocol—no more difficult than E. coli expression

Eliminate the hassle of working with baculovirus insect expression systems or creating stable mammalian cell lines. The Expi293 Expression System is quick and easy and is designed to provide ultrahigh protein yields in just a week (Figure 3).


Figure 3. Expi293 Expression System protocol.


Expi293 Protein Expression Data

The table below highlights yields of various proteins produced by the Expi293 Expression System.

See full list of expression data

Description Gene symbol Accession number Expi293 system yield (mg/L)
Plekstrin, mRNA (cDNA clone MGC:17111 IMAGE:4341823), complete CDS PLEK AAH1549.1 5,610
Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3), transcript variant 1, mRNA STAT3 NP_644805.1 2,139
BH3 interacting domain death agonist (BID), transcript variant 2, mRNA BID NP_001187.1 972
RAB38, member RAS oncogene family (RAB38), mRNA RAB38 NP_071732.1 702
S100 calcium binding protein A4 (S100A4), transcript variant 1, mRNA S100A4 NP_002952.1 609
NAD(P)H dehydrogenase, quinone 1 (NQ01) transcript variant 1, mRNA NQ01 NP_000894.1 525
Prosaposin (PSAP), transcript variant 1, mRNA PSAP NP_002769.1 524