Protein Expression Protocol Calculator

We’ll do the calculations for you, using our protein expression protocol calculator

Save time and hassle with our interactive protein expression protocol calculator for the Gibco ExpiCHO and Expi293 Expression Systems. Simply input a few details about your planned experiment, and we’ll do the calculations for you and provide a customized one-page PDF protocol for your convenience.

How to use the expression calculator

  1. Select ExpiCHO or Expi293 expression system.
  2. Enter details about your cell culture preparation such as volume of culture to be transfected (mL), number of wells to be transfected, nominal volume of cell suspension 96 x 106/ml required (mL), and shaking diameter (mm).
  3. Enter specifics about your transfection setup.
  4. Get enhancer volumes (and feed volumes, if applicable) for your specific protocol.
  5. Download your finalized protocol to PDF and print, if desired.

Need information on alternative protein expression systems? Learn more

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For Research Use Only. Not for use in diagnostic procedures.