Eukaryotic protein expression
Baculovirus expression systems offer high-level expression, ease of scale-up, and production of recombinant protein with posttranslational modifications.
When a functional target protein is required and other expression systems are not amenable, we offer a baculovirus expression option in two proprietary insect cell lines—Sf9 and Sf21.
A titerless viral supernatant infection method helps to ensure that the optimal host and expression conditions are identified in real time and reproducible in scale up to 200 L.
For inquiries about our protein expression services, contact firstname.lastname@example.org.
Small-scale expression studies
A viral supernatant dilution scheme of at least three different volumes is added to 50 mL of Sf9 and Sf21 insect cell cultures. Standardized cell culture aliquots are removed at 0, 24, 48, and 72 hours and evaluated for cell density, viability, and size over time. The optimal expression conditions are identified via western blot analysis.
Protein purification services
Affinity, ion exchange, HIC, SEC, and other purification methods employed
Generation of recombinant bacmid and high-titer viral supernatant
From 1 L to 200 L
What advantages does a baculovirus expression system provide?
Baculovirus expression systems can be used when bacterial expression is not feasible within a bacterial expression system, and also when a target protein with posttranslational modifications is needed for functional and structural studies. We recommend that you start with a GeneArt® expression-optimized gene, which can improve expression and subsequent protein yield.
Is baculovirus infectious to humans?
No, it is completely safe but still enables protein expression within a eukaryotic system.
How long does a baculovirus expression project usually take?
Most services, starting with the baculovirus expression plasmid, can be completed in 6–7 weeks (including purification).
For Research Use Only. Not for use in diagnostic procedures.