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Rapid expression of functional protein

Producing a functional mammalian protein often necessitates using a mammalian host to ensure proper posttranslational modification and function. Protein expression in our proprietary serum-free Expi293™, FreeStyle™ 293, and FreeStyle™ CHO-S suspension systems allows for rapid production of functional, soluble proteins.

  • Small-scale expression studies—multiple expression time points can be evaluated in Expi293™, FreeStyle™ 293, and FreeStyle™ CHO-S cell lines simultaneously, to quickly optimize your service expression conditions
  • Expression scale-up—from 1 L to 200 L
  • Protein purification services—affinity, ion exchange, HIC, SEC, and other purification methods are employed

For inquiries about our protein expression services, contact custom.services@lifetech.com.

FAQs

What do I need to provide as starting material?

We can use the expression vector you already have, or we can begin the project by constructing the expression vector for you. We recommend that you start with a GeneArt® expression-optimized gene, which can improve expression and subsequent protein yield.

How long does the service take?

Most mammalian expression services projects are completed in 5–6 weeks (including purification).

What does the service deliver?

You will receive purified protein and a comprehensive data report covering the expression and purification.