photo of the Bolt Mini Gel Tank  

Find resources for the original Bolt® Mini Gel Tank including FAQs, how to upgrade your Bolt Mini Gel Tank for versatility, instructional videos for how to use your Bolt Mini Gel Tank with NuPAGE gels, Novex gels, and the new Bolt gels.

If you own an original Bolt® Mini Gel Tank, you can request a free set of replacement cam handles (Cat. No. A26732) that will facilitate use of the new 10 cm Bolt® mini gels.

Equal performance of the original Bolt 10.5 cm gels in the original Bolt mini gel tank and the new Bolt 10 cm gels in the Bolt mini gel tank with the new cam handles.

Equal performance of a new Bolt 4–12% Bis-Tris Plus gel with protein standards and samples. The same protein standards and samples were loaded in 10 µL sample volumes in a new Bolt gel and an original Bolt gel. Similarly sharp, straight bands with nearly identical migration were observed with the new Bolt gel (A) as compared to the original Bolt gel (B). Lane 1: SeeBlue Plus2 Pre-Stained Standard; lane 2: 10 µg E. coli lysate; lane 3: blend of 12 purified proteins (Mark12 Unstained Standard); lane 4: 40 µg HeLa cell lysate; lane 5: 20 µg HeLa cell lysate; lane 6: 5 µg BSA; lane 7: 40 µg Jurkat cell lysate; lane 8: 5 µg of a GST fusion protein; lane 9: Novex Sharp Protein Standard; and lane 10: 5 µg β-galactosidase. Gel electrophoresis was performed at 200 V (constant), gels were stained using SimplyBlue SafeStain, and images were acquired using a flatbed scanner.

New vs old bolt tank

Q. What is the difference between the original bolt mini gel tank and the upgraded bolt mini gel tank with the new cam handles?
A. The table below explains the differences.

 Original Bolt Mini Gel Tank with original cam handlesEnhanced Bolt Mini Gel Tank with replacement cam handles
Easy sample loading with forward-facing well configuration

Less running buffer required

Side-by-side simultaneous visualization of two gels

Simplified monitoring of pre-stained protein markers against a white tank stand

Compatible mini protein gels
Original Bolt Bis-Tris Plus gels (10.5 cm)

 
New Bolt Bis-Tris Plus gels (10 cm) 

NuPAGE Tris-Acetate Protein gels 

NuPAGE Bis-Tris Protein gels 

Novex Tris-Glycine Mini Protein gels 

Novex Tricine Mini Protein gels 

Q. What exactly comes with the new tank?
A. The new tank will include the exact same components as the old tank with the following exceptions: 2 new cam handles sized for 10 cm gels, an updated manual, and a special insert describing the changes to the tank compared to the original one.

Q. Which mini gels will run* in the tank with the 10 cm cam handle?
A.

  • NuPAGE® Tris-Acetate Protein Gels
  • New 10 cm Bolt® Bis-Tris Plus Gels
  • NuPAGE® Bis-Tris Protein Gels
  • Novex® Tris-Glycine Mini Protein Gels
  • Novex ®Tricine Protein Gels

*Refer to the user manual for specific run conditions for each.

Q. In what way have the Bolt® gels changed?
A. The original Bolt® mini gels were 10.5 cm. The new Bolt® gels will be 10 cm.

Q. What is the name of the new gel? What is the price?
A. Even though the size has changed (to 10 cm), the mini gels will keep their current name: Bolt® Bis-Tris Plus Gel. There is no price difference between the original Bolt® gels and the new Bolt® gels.

Q. Can Bio-Rad gels run in the Bolt® Mini Gel Tank with the new cam handles?
A. No. Bio-Rad gels are a different size (length, width, and depth) and have a special feature that precludes them from fitting in the Bolt® Mini Gel Tank.

Q. Is there a wet transfer western blot module available?
A. Yes, the Mini Blot Module.

Q. Which power supplies are compatible with the tank?
A. The tank is compatible with the Life Technologies™ PowerEase® and Zoom® power supplies as well as Bio-Rad, Thermo Fisher Scientific, and Hoefer power supplies. Novex® power supply adapters are supplied for power supplies not designed for use with covered or retractable power leads.

If you own an original Bolt® Mini Gel Tank, you can request a free set of replacement cam handles (Cat. No. A26732) that will facilitate use of the new 10 cm Bolt® mini gels.

Old Cat. No.ProductQuantityNew Cat. No.ProductQuantity
B0412ABolt Welcome Pack A (4 – 12%, 10 well) 1 kitNW0412ABolt Welcome Pack A (4 – 12%, 10 well) 1 kit
B0412AIB2Bolt Welcome Pack + iBlot 2 System 1 kitNW0412AIB2Bolt Welcome Pack + iBlot 2 System 1 kit
B0412BBolt Welcome Pack B (4 – 12%, 15 well) 1 kitNW0412BBolt Welcome Pack B (4 – 12%, 15 well) 1 kit
B2000Bolt Tank and Blot Module Combo 1 kitNW2000Mini Gel Tank and Blot Module Set1 kit
B4478592Cassette Clamp, right1 unitA25945Cassette Clamp, right1 unit
B4478593Cassette Clamp, left1 unitA25946Cassette Clamp, left1 unit
B4477599Bolt Mini Gel Tank 1 unitA25977Mini Gel Tank 1 unit
B4478591Bolt Tank Lid1 unitA25944Tank Lid1 unit
B4478640Bolt Tank Base1 unitA25950Tank Base1 unit
BG00080BOXBolt 8% Bis-Tris Plus Gel, 10 Well10 gelsNW00080BOXBolt 8% Bis-Tris Plus Gel, 10 Well10 gels
BG00082BOXBolt 8% Bis-Tris Plus Gel, 12 Well10 gelsNW00082BOXBolt 8% Bis-Tris Plus Gel, 12 Well10 gels
BG00085BOXBolt 8% Bis-Tris Plus Gel, 15 Well10 gelsNW00085BOXBolt 8% Bis-Tris Plus Gel, 15 Well10 gels
BG00087BOXBolt 8% Bis-Tris Plus Gel, 17 Well10 gelsNW00087BOXBolt 8% Bis-Tris Plus Gel, 17 Well10 gels
BG00100BOXBolt 10% Bis-Tris Plus Gel, 10 Well10 gelsNW00100BOXBolt 10% Bis-Tris Plus Gel, 10 Well10 gels
BG00102BOXBolt 10% Bis-Tris Plus Gel, 12 Well10 gelsNW00102BOXBolt 10% Bis-Tris Plus Gel, 12 Well10 gels
BG00105BOXBolt 10% Bis-Tris Plus Gel, 15 Well10 gelsNW00105BOXBolt 10% Bis-Tris Plus Gel, 15 Well10 gels
BG00107BOXBolt 10% Bis-Tris Plus Gel, 17 Well10 gelsNW00107BOXBolt 10% Bis-Tris Plus Gel, 17 Well10 gels
BG00120BOXBolt 12% Bis-Tris Plus Gel, 10 Well10 gelsNW00120BOXBolt 12% Bis-Tris Plus Gel, 10 Well10 gels
BG00122BOXBolt 12% Bis-Tris Plus Gel, 12 Well10 gelsNW00122BOXBolt 12% Bis-Tris Plus Gel, 12 Well10 gels
BG00125BOXBolt 12% Bis-Tris Plus Gel, 15 Well10 gelsNW00125BOXBolt 12% Bis-Tris Plus Gel, 15 Well10 gels
BG00127BOXBolt 12% Bis-Tris Plus Gel, 17 Well10 gelsNW00127BOXBolt 12% Bis-Tris Plus Gel, 17 Well10 gels
BG04120BOXBolt 4 – 12% Bis-Tris Plus Gel, 10 Well10 gelsNW04120BOXBolt 4 – 12% Bis-Tris Plus Gel, 10 Well10 gels
BG04122BOXBolt 4 – 12% Bis-Tris Plus Gel, 12 Well10 gelsNW04122BOXBolt 4 – 12% Bis-Tris Plus Gel, 12 Well10 gels
BG04125BOXBolt 4 – 12% Bis-Tris Plus Gel, 15 Well10 gelsNW04125BOXBolt 4 – 12% Bis-Tris Plus Gel, 15 Well10 gels
BG04127BOXBolt 4 – 12% Bis-Tris Plus Gel, 17 Well10 gelsNW04127BOXBolt 4 – 12% Bis-Tris Plus Gel, 17 Well10 gels
BT2010Bolt Empty Gel Cassettes20 cassettesNW2010Bolt Empty Mini Gel Cassettes20 cassettes
BT3010Bolt Empty Gel Cassette Combs, 10 Well20 combsNW3010Bolt Empty Mini Gel Cassette Combs, 10 Well20 combs
BT3012Bolt Empty Gel Cassette Combs, 12 Well20 combsNW3012Bolt Empty Mini Gel Cassette Combs, 12 Well20 combs
NANANAA26732Cam Handles Kit 2 units

Equal performance of the original Bolt 10.5 cm gels in the original Bolt mini gel tank and the new Bolt 10 cm gels in the Bolt mini gel tank with the new cam handles.

Equal performance of a new Bolt 4–12% Bis-Tris Plus gel with protein standards and samples. The same protein standards and samples were loaded in 10 µL sample volumes in a new Bolt gel and an original Bolt gel. Similarly sharp, straight bands with nearly identical migration were observed with the new Bolt gel (A) as compared to the original Bolt gel (B). Lane 1: SeeBlue Plus2 Pre-Stained Standard; lane 2: 10 µg E. coli lysate; lane 3: blend of 12 purified proteins (Mark12 Unstained Standard); lane 4: 40 µg HeLa cell lysate; lane 5: 20 µg HeLa cell lysate; lane 6: 5 µg BSA; lane 7: 40 µg Jurkat cell lysate; lane 8: 5 µg of a GST fusion protein; lane 9: Novex Sharp Protein Standard; and lane 10: 5 µg β-galactosidase. Gel electrophoresis was performed at 200 V (constant), gels were stained using SimplyBlue SafeStain, and images were acquired using a flatbed scanner.

New vs old bolt tank

Q. What is the difference between the original bolt mini gel tank and the upgraded bolt mini gel tank with the new cam handles?
A. The table below explains the differences.

 Original Bolt Mini Gel Tank with original cam handlesEnhanced Bolt Mini Gel Tank with replacement cam handles
Easy sample loading with forward-facing well configuration

Less running buffer required

Side-by-side simultaneous visualization of two gels

Simplified monitoring of pre-stained protein markers against a white tank stand

Compatible mini protein gels
Original Bolt Bis-Tris Plus gels (10.5 cm)

 
New Bolt Bis-Tris Plus gels (10 cm) 

NuPAGE Tris-Acetate Protein gels 

NuPAGE Bis-Tris Protein gels 

Novex Tris-Glycine Mini Protein gels 

Novex Tricine Mini Protein gels 

Q. What exactly comes with the new tank?
A. The new tank will include the exact same components as the old tank with the following exceptions: 2 new cam handles sized for 10 cm gels, an updated manual, and a special insert describing the changes to the tank compared to the original one.

Q. Which mini gels will run* in the tank with the 10 cm cam handle?
A.

  • NuPAGE® Tris-Acetate Protein Gels
  • New 10 cm Bolt® Bis-Tris Plus Gels
  • NuPAGE® Bis-Tris Protein Gels
  • Novex® Tris-Glycine Mini Protein Gels
  • Novex ®Tricine Protein Gels

*Refer to the user manual for specific run conditions for each.

Q. In what way have the Bolt® gels changed?
A. The original Bolt® mini gels were 10.5 cm. The new Bolt® gels will be 10 cm.

Q. What is the name of the new gel? What is the price?
A. Even though the size has changed (to 10 cm), the mini gels will keep their current name: Bolt® Bis-Tris Plus Gel. There is no price difference between the original Bolt® gels and the new Bolt® gels.

Q. Can Bio-Rad gels run in the Bolt® Mini Gel Tank with the new cam handles?
A. No. Bio-Rad gels are a different size (length, width, and depth) and have a special feature that precludes them from fitting in the Bolt® Mini Gel Tank.

Q. Is there a wet transfer western blot module available?
A. Yes, the Mini Blot Module.

Q. Which power supplies are compatible with the tank?
A. The tank is compatible with the Life Technologies™ PowerEase® and Zoom® power supplies as well as Bio-Rad, Thermo Fisher Scientific, and Hoefer power supplies. Novex® power supply adapters are supplied for power supplies not designed for use with covered or retractable power leads.

If you own an original Bolt® Mini Gel Tank, you can request a free set of replacement cam handles (Cat. No. A26732) that will facilitate use of the new 10 cm Bolt® mini gels.

Old Cat. No.ProductQuantityNew Cat. No.ProductQuantity
B0412ABolt Welcome Pack A (4 – 12%, 10 well) 1 kitNW0412ABolt Welcome Pack A (4 – 12%, 10 well) 1 kit
B0412AIB2Bolt Welcome Pack + iBlot 2 System 1 kitNW0412AIB2Bolt Welcome Pack + iBlot 2 System 1 kit
B0412BBolt Welcome Pack B (4 – 12%, 15 well) 1 kitNW0412BBolt Welcome Pack B (4 – 12%, 15 well) 1 kit
B2000Bolt Tank and Blot Module Combo 1 kitNW2000Mini Gel Tank and Blot Module Set1 kit
B4478592Cassette Clamp, right1 unitA25945Cassette Clamp, right1 unit
B4478593Cassette Clamp, left1 unitA25946Cassette Clamp, left1 unit
B4477599Bolt Mini Gel Tank 1 unitA25977Mini Gel Tank 1 unit
B4478591Bolt Tank Lid1 unitA25944Tank Lid1 unit
B4478640Bolt Tank Base1 unitA25950Tank Base1 unit
BG00080BOXBolt 8% Bis-Tris Plus Gel, 10 Well10 gelsNW00080BOXBolt 8% Bis-Tris Plus Gel, 10 Well10 gels
BG00082BOXBolt 8% Bis-Tris Plus Gel, 12 Well10 gelsNW00082BOXBolt 8% Bis-Tris Plus Gel, 12 Well10 gels
BG00085BOXBolt 8% Bis-Tris Plus Gel, 15 Well10 gelsNW00085BOXBolt 8% Bis-Tris Plus Gel, 15 Well10 gels
BG00087BOXBolt 8% Bis-Tris Plus Gel, 17 Well10 gelsNW00087BOXBolt 8% Bis-Tris Plus Gel, 17 Well10 gels
BG00100BOXBolt 10% Bis-Tris Plus Gel, 10 Well10 gelsNW00100BOXBolt 10% Bis-Tris Plus Gel, 10 Well10 gels
BG00102BOXBolt 10% Bis-Tris Plus Gel, 12 Well10 gelsNW00102BOXBolt 10% Bis-Tris Plus Gel, 12 Well10 gels
BG00105BOXBolt 10% Bis-Tris Plus Gel, 15 Well10 gelsNW00105BOXBolt 10% Bis-Tris Plus Gel, 15 Well10 gels
BG00107BOXBolt 10% Bis-Tris Plus Gel, 17 Well10 gelsNW00107BOXBolt 10% Bis-Tris Plus Gel, 17 Well10 gels
BG00120BOXBolt 12% Bis-Tris Plus Gel, 10 Well10 gelsNW00120BOXBolt 12% Bis-Tris Plus Gel, 10 Well10 gels
BG00122BOXBolt 12% Bis-Tris Plus Gel, 12 Well10 gelsNW00122BOXBolt 12% Bis-Tris Plus Gel, 12 Well10 gels
BG00125BOXBolt 12% Bis-Tris Plus Gel, 15 Well10 gelsNW00125BOXBolt 12% Bis-Tris Plus Gel, 15 Well10 gels
BG00127BOXBolt 12% Bis-Tris Plus Gel, 17 Well10 gelsNW00127BOXBolt 12% Bis-Tris Plus Gel, 17 Well10 gels
BG04120BOXBolt 4 – 12% Bis-Tris Plus Gel, 10 Well10 gelsNW04120BOXBolt 4 – 12% Bis-Tris Plus Gel, 10 Well10 gels
BG04122BOXBolt 4 – 12% Bis-Tris Plus Gel, 12 Well10 gelsNW04122BOXBolt 4 – 12% Bis-Tris Plus Gel, 12 Well10 gels
BG04125BOXBolt 4 – 12% Bis-Tris Plus Gel, 15 Well10 gelsNW04125BOXBolt 4 – 12% Bis-Tris Plus Gel, 15 Well10 gels
BG04127BOXBolt 4 – 12% Bis-Tris Plus Gel, 17 Well10 gelsNW04127BOXBolt 4 – 12% Bis-Tris Plus Gel, 17 Well10 gels
BT2010Bolt Empty Gel Cassettes20 cassettesNW2010Bolt Empty Mini Gel Cassettes20 cassettes
BT3010Bolt Empty Gel Cassette Combs, 10 Well20 combsNW3010Bolt Empty Mini Gel Cassette Combs, 10 Well20 combs
BT3012Bolt Empty Gel Cassette Combs, 12 Well20 combsNW3012Bolt Empty Mini Gel Cassette Combs, 12 Well20 combs
NANANAA26732Cam Handles Kit 2 units