photo of the Bolt Mini Gel Tank   

Find resources for the original Bolt® Mini Gel Tank including FAQs, how to upgrade your Bolt® Mini Gel Tank for versatility, instructional videos for how to use your Bolt® Mini Gel Tank with NuPAGE® gels, Novex® gels, and the new Bolt® gels.

If you own an original Bolt® Mini Gel Tank, you can request a free set of replacement cam handles (Cat. No. A26732) that will facilitate use of the new 10 cm Bolt® mini gels.

Equal performance of the original Bolt® 10.5 cm gels in the original Bolt® mini gel tank and the new Bolt® 10 cm gels in the Bolt® mini gel tank with the new cam handles.

Equal performance of a new Bolt® 4–12% Bis-Tris Plus gel with protein standards and samples. The same protein standards and samples were loaded in 10 µL sample volumes in a new Bolt® gel and an original Bolt® gel. Similarly sharp, straight bands with nearly identical migration were observed with the new Bolt® gel (A) as compared to the original Bolt® gel (B). Lane 1: SeeBlue® Plus2 Pre-Stained Standard; lane 2: 10 µg E. coli lysate; lane 3: blend of 12 purified proteins (Mark12™ Unstained Standard); lane 4: 40 µg HeLa cell lysate; lane 5: 20 µg HeLa cell lysate; lane 6: 5 µg BSA; lane 7: 40 µg Jurkat cell lysate; lane 8: 5 µg of a GST fusion protein; lane 9: Novex® Sharp Protein Standard; and lane 10: 5 µg β-galactosidase. Gel electrophoresis was performed at 200 V (constant), gels were stained using SimplyBlue™ SafeStain, and images were acquired using a flatbed scanner.

photo of original Bolt gel compared to new Bolt gel

Q. What is the difference between the original bolt mini gel tank and the upgraded bolt mini gel tank with the new cam handles?
A. The table below explains the differences.

Original Bolt® Mini Gel Tank with original cam handles Enhanced Bolt® Mini Gel Tank with replacement cam handles
Easy sample loading with forward-facing well configuration

Less running buffer required

Side-by-side simultaneous visualization of two gels

Simplified monitoring of pre-stained protein markers against a white tank stand

Compatible mini protein gels
Original Bolt® Bis-Tris Plus gels (10.5 cm)

New Bolt® Bis-Tris Plus gels (10 cm)

NuPAGE® Tris-Acetate Protein gels

NuPAGE® Bis-Tris Protein gels

Novex® Tris-Glycine Mini Protein gels

Novex® Tricine Mini Protein gels

Q. What exactly comes with the new tank?
A. The new tank will include the exact same components as the old tank with the following exceptions: 2 new cam handles sized for 10 cm gels, an updated manual, and a special insert describing the changes to the tank compared to the original one.

Q. Which mini gels will run* in the tank with the 10 cm cam handle?
A.

  • NuPAGE® Tris-Acetate Protein Gels
  • New 10 cm Bolt® Bis-Tris Plus Gels
  • NuPAGE® Bis-Tris Protein Gels
  • Novex® Tris-Glycine Mini Protein Gels
  • Novex ®Tricine Protein Gels

*Refer to the user manual for specific run conditions for each.

Q. In what way have the Bolt® gels changed?
A. The original Bolt® mini gels were 10.5 cm. The new Bolt® gels will be 10 cm.

Q. What is the name of the new gel? What is the price?
A. Even though the size has changed (to 10 cm), the mini gels will keep their current name: Bolt® Bis-Tris Plus Gel. There is no price difference between the original Bolt® gels and the new Bolt® gels.

Q. Can Bio-Rad gels run in the Bolt® Mini Gel Tank with the new cam handles?
A. No. Bio-Rad gels are a different size (length, width, and depth) and have a special feature that precludes them from fitting in the Bolt® Mini Gel Tank.

Q. Is there a wet transfer western blot module available?
A. Yes, the Mini Blot Module.

Q. Which power supplies are compatible with the tank?
A. The tank is compatible with the Life Technologies™ PowerEase® and Zoom® power supplies as well as Bio-Rad, Thermo Fisher Scientific, and Hoefer power supplies. Novex® power supply adapters are supplied for power supplies not designed for use with covered or retractable power leads.

If you own an original Bolt® Mini Gel Tank, you can request a free set of replacement cam handles (Cat. No. A26732) that will facilitate use of the new 10 cm Bolt® mini gels.

Old Cat. No. Product Quantity New Cat. No. Product Quantity
B0412A Bolt® Welcome Pack A (4 – 12%, 10 well)  1 kit NW0412A Bolt® Welcome Pack A (4 – 12%, 10 well)  1 kit
B0412AIB2 Bolt® Welcome Pack + iBlot® 2 System  1 kit NW0412AIB2 Bolt® Welcome Pack + iBlot® 2 System  1 kit
B0412B Bolt® Welcome Pack B (4 – 12%, 15 well)  1 kit NW0412B Bolt® Welcome Pack B (4 – 12%, 15 well)  1 kit
B2000 Bolt® Tank and Blot Module Combo  1 kit NW2000 Mini Gel Tank and Blot Module Set 1 kit
B4478592 Cassette Clamp, right 1 unit A25945 Cassette Clamp, right 1 unit
B4478593 Cassette Clamp, left 1 unit A25946 Cassette Clamp, left 1 unit
B4477599 Bolt® Mini Gel Tank  1 unit A25977 Mini Gel Tank  1 unit
B4478591 Bolt® Tank Lid 1 unit A25944 Tank Lid 1 unit
B4478640 Bolt® Tank Base 1 unit A25950 Tank Base 1 unit
BG00080BOX Bolt® 8% Bis-Tris Plus Gel, 10 Well 10 gels NW00080BOX Bolt® 8% Bis-Tris Plus Gel, 10 Well 10 gels
BG00082BOX Bolt® 8% Bis-Tris Plus Gel, 12 Well 10 gels NW00082BOX Bolt® 8% Bis-Tris Plus Gel, 12 Well 10 gels
BG00085BOX Bolt® 8% Bis-Tris Plus Gel, 15 Well 10 gels NW00085BOX Bolt® 8% Bis-Tris Plus Gel, 15 Well 10 gels
BG00087BOX Bolt® 8% Bis-Tris Plus Gel, 17 Well 10 gels NW00087BOX Bolt® 8% Bis-Tris Plus Gel, 17 Well 10 gels
BG00100BOX Bolt® 10% Bis-Tris Plus Gel, 10 Well 10 gels NW00100BOX Bolt® 10% Bis-Tris Plus Gel, 10 Well 10 gels
BG00102BOX Bolt® 10% Bis-Tris Plus Gel, 12 Well 10 gels NW00102BOX Bolt® 10% Bis-Tris Plus Gel, 12 Well 10 gels
BG00105BOX Bolt® 10% Bis-Tris Plus Gel, 15 Well 10 gels NW00105BOX Bolt® 10% Bis-Tris Plus Gel, 15 Well 10 gels
BG00107BOX Bolt® 10% Bis-Tris Plus Gel, 17 Well 10 gels NW00107BOX Bolt® 10% Bis-Tris Plus Gel, 17 Well 10 gels
BG00120BOX Bolt® 12% Bis-Tris Plus Gel, 10 Well 10 gels NW00120BOX Bolt® 12% Bis-Tris Plus Gel, 10 Well 10 gels
BG00122BOX Bolt® 12% Bis-Tris Plus Gel, 12 Well 10 gels NW00122BOX Bolt® 12% Bis-Tris Plus Gel, 12 Well 10 gels
BG00125BOX Bolt® 12% Bis-Tris Plus Gel, 15 Well 10 gels NW00125BOX Bolt® 12% Bis-Tris Plus Gel, 15 Well 10 gels
BG00127BOX Bolt® 12% Bis-Tris Plus Gel, 17 Well 10 gels NW00127BOX Bolt® 12% Bis-Tris Plus Gel, 17 Well 10 gels
BG04120BOX Bolt® 4 – 12% Bis-Tris Plus Gel, 10 Well 10 gels NW04120BOX Bolt® 4 – 12% Bis-Tris Plus Gel, 10 Well 10 gels
BG04122BOX Bolt® 4 – 12% Bis-Tris Plus Gel, 12 Well 10 gels NW04122BOX Bolt® 4 – 12% Bis-Tris Plus Gel, 12 Well 10 gels
BG04125BOX Bolt® 4 – 12% Bis-Tris Plus Gel, 15 Well 10 gels NW04125BOX Bolt® 4 – 12% Bis-Tris Plus Gel, 15 Well 10 gels
BG04127BOX Bolt® 4 – 12% Bis-Tris Plus Gel, 17 Well 10 gels NW04127BOX Bolt® 4 – 12% Bis-Tris Plus Gel, 17 Well 10 gels
BT2010 Bolt® Empty Gel Cassettes 20 cassettes NW2010 Bolt® Empty Mini Gel Cassettes 20 cassettes
BT3010 Bolt® Empty Gel Cassette Combs, 10 Well 20 combs NW3010 Bolt® Empty Mini Gel Cassette Combs, 10 Well 20 combs
BT3012 Bolt® Empty Gel Cassette Combs, 12 Well 20 combs NW3012 Bolt® Empty Mini Gel Cassette Combs, 12 Well 20 combs
NA NA NA A26732 Cam Handles Kit  2 units