Resources for Upgrading the Bolt® Mini Gel Tank
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Find resources for the original Bolt® Mini Gel Tank including FAQs, how to upgrade your Bolt® Mini Gel Tank for versatility, instructional videos for how to use your Bolt® Mini Gel Tank with NuPAGE® gels, Novex® gels, and the new Bolt® gels. |
If you own an original Bolt® Mini Gel Tank, you can request a free set of replacement cam handles (Cat. No. A26732) that will facilitate use of the new 10 cm Bolt® mini gels.
Equal performance of the original Bolt® 10.5 cm gels in the original Bolt® mini gel tank and the new Bolt® 10 cm gels in the Bolt® mini gel tank with the new cam handles.
Equal performance of a new Bolt® 4–12% Bis-Tris Plus gel with protein standards and samples. The same protein standards and samples were loaded in 10 µL sample volumes in a new Bolt® gel and an original Bolt® gel. Similarly sharp, straight bands with nearly identical migration were observed with the new Bolt® gel (A) as compared to the original Bolt® gel (B). Lane 1: SeeBlue® Plus2 Pre-Stained Standard; lane 2: 10 µg E. coli lysate; lane 3: blend of 12 purified proteins (Mark12™ Unstained Standard); lane 4: 40 µg HeLa cell lysate; lane 5: 20 µg HeLa cell lysate; lane 6: 5 µg BSA; lane 7: 40 µg Jurkat cell lysate; lane 8: 5 µg of a GST fusion protein; lane 9: Novex® Sharp Protein Standard; and lane 10: 5 µg β-galactosidase. Gel electrophoresis was performed at 200 V (constant), gels were stained using SimplyBlue™ SafeStain, and images were acquired using a flatbed scanner.
Q. What is the difference between the original bolt mini gel tank and the upgraded bolt mini gel tank with the new cam handles?
A. The table below explains the differences.
| Original Bolt® Mini Gel Tank with original cam handles | Enhanced Bolt® Mini Gel Tank with replacement cam handles | |
|---|---|---|
| Easy sample loading with forward-facing well configuration | ✓ |
✓ |
| Less running buffer required | ✓ |
✓ |
| Side-by-side simultaneous visualization of two gels | ✓ |
✓ |
| Simplified monitoring of pre-stained protein markers against a white tank stand | ✓ |
✓ |
| Compatible mini protein gels | ||
| Original Bolt® Bis-Tris Plus gels (10.5 cm) | ✓ |
|
| New Bolt® Bis-Tris Plus gels (10 cm) | ✓ |
|
| NuPAGE® Tris-Acetate Protein gels | ✓ |
|
| NuPAGE® Bis-Tris Protein gels | ✓ |
|
| Novex® Tris-Glycine Mini Protein gels | ✓ |
|
| Novex® Tricine Mini Protein gels | ✓ |
|
Q. What exactly comes with the new tank?
A. The new tank will include the exact same components as the old tank with the following exceptions: 2 new cam handles sized for 10 cm gels, an updated manual, and a special insert describing the changes to the tank compared to the original one.
Q. Which mini gels will run* in the tank with the 10 cm cam handle?
A.
- NuPAGE® Tris-Acetate Protein Gels
- New 10 cm Bolt® Bis-Tris Plus Gels
- NuPAGE® Bis-Tris Protein Gels
- Novex® Tris-Glycine Mini Protein Gels
- Novex ®Tricine Protein Gels
*Refer to the user manual for specific run conditions for each.
Q. In what way have the Bolt® gels changed?
A. The original Bolt® mini gels were 10.5 cm. The new Bolt® gels will be 10 cm.
Q. What is the name of the new gel? What is the price?
A. Even though the size has changed (to 10 cm), the mini gels will keep their current name: Bolt® Bis-Tris Plus Gel. There is no price difference between the original Bolt® gels and the new Bolt® gels.
Q. Can Bio-Rad gels run in the Bolt® Mini Gel Tank with the new cam handles?
A. No. Bio-Rad gels are a different size (length, width, and depth) and have a special feature that precludes them from fitting in the Bolt® Mini Gel Tank.
Q. Is there a wet transfer western blot module available?
A. Yes, the Mini Blot Module.
Q. Which power supplies are compatible with the tank?
A. The tank is compatible with the Life Technologies™ PowerEase® and Zoom® power supplies as well as Bio-Rad, Thermo Fisher Scientific, and Hoefer power supplies. Novex® power supply adapters are supplied for power supplies not designed for use with covered or retractable power leads.
If you own an original Bolt® Mini Gel Tank, you can request a free set of replacement cam handles (Cat. No. A26732) that will facilitate use of the new 10 cm Bolt® mini gels.
| Old Cat. No. | Product | Quantity | New Cat. No. | Product | Quantity |
|---|---|---|---|---|---|
| B0412A | Bolt® Welcome Pack A (4 – 12%, 10 well) | 1 kit | NW0412A | Bolt® Welcome Pack A (4 – 12%, 10 well) | 1 kit |
| B0412AIB2 | Bolt® Welcome Pack + iBlot® 2 System | 1 kit | NW0412AIB2 | Bolt® Welcome Pack + iBlot® 2 System | 1 kit |
| B0412B | Bolt® Welcome Pack B (4 – 12%, 15 well) | 1 kit | NW0412B | Bolt® Welcome Pack B (4 – 12%, 15 well) | 1 kit |
| B2000 | Bolt® Tank and Blot Module Combo | 1 kit | NW2000 | Mini Gel Tank and Blot Module Set | 1 kit |
| B4478592 | Cassette Clamp, right | 1 unit | A25945 | Cassette Clamp, right | 1 unit |
| B4478593 | Cassette Clamp, left | 1 unit | A25946 | Cassette Clamp, left | 1 unit |
| B4477599 | Bolt® Mini Gel Tank | 1 unit | A25977 | Mini Gel Tank | 1 unit |
| B4478591 | Bolt® Tank Lid | 1 unit | A25944 | Tank Lid | 1 unit |
| B4478640 | Bolt® Tank Base | 1 unit | A25950 | Tank Base | 1 unit |
| BG00080BOX | Bolt® 8% Bis-Tris Plus Gel, 10 Well | 10 gels | NW00080BOX | Bolt® 8% Bis-Tris Plus Gel, 10 Well | 10 gels |
| BG00082BOX | Bolt® 8% Bis-Tris Plus Gel, 12 Well | 10 gels | NW00082BOX | Bolt® 8% Bis-Tris Plus Gel, 12 Well | 10 gels |
| BG00085BOX | Bolt® 8% Bis-Tris Plus Gel, 15 Well | 10 gels | NW00085BOX | Bolt® 8% Bis-Tris Plus Gel, 15 Well | 10 gels |
| BG00087BOX | Bolt® 8% Bis-Tris Plus Gel, 17 Well | 10 gels | NW00087BOX | Bolt® 8% Bis-Tris Plus Gel, 17 Well | 10 gels |
| BG00100BOX | Bolt® 10% Bis-Tris Plus Gel, 10 Well | 10 gels | NW00100BOX | Bolt® 10% Bis-Tris Plus Gel, 10 Well | 10 gels |
| BG00102BOX | Bolt® 10% Bis-Tris Plus Gel, 12 Well | 10 gels | NW00102BOX | Bolt® 10% Bis-Tris Plus Gel, 12 Well | 10 gels |
| BG00105BOX | Bolt® 10% Bis-Tris Plus Gel, 15 Well | 10 gels | NW00105BOX | Bolt® 10% Bis-Tris Plus Gel, 15 Well | 10 gels |
| BG00107BOX | Bolt® 10% Bis-Tris Plus Gel, 17 Well | 10 gels | NW00107BOX | Bolt® 10% Bis-Tris Plus Gel, 17 Well | 10 gels |
| BG00120BOX | Bolt® 12% Bis-Tris Plus Gel, 10 Well | 10 gels | NW00120BOX | Bolt® 12% Bis-Tris Plus Gel, 10 Well | 10 gels |
| BG00122BOX | Bolt® 12% Bis-Tris Plus Gel, 12 Well | 10 gels | NW00122BOX | Bolt® 12% Bis-Tris Plus Gel, 12 Well | 10 gels |
| BG00125BOX | Bolt® 12% Bis-Tris Plus Gel, 15 Well | 10 gels | NW00125BOX | Bolt® 12% Bis-Tris Plus Gel, 15 Well | 10 gels |
| BG00127BOX | Bolt® 12% Bis-Tris Plus Gel, 17 Well | 10 gels | NW00127BOX | Bolt® 12% Bis-Tris Plus Gel, 17 Well | 10 gels |
| BG04120BOX | Bolt® 4 – 12% Bis-Tris Plus Gel, 10 Well | 10 gels | NW04120BOX | Bolt® 4 – 12% Bis-Tris Plus Gel, 10 Well | 10 gels |
| BG04122BOX | Bolt® 4 – 12% Bis-Tris Plus Gel, 12 Well | 10 gels | NW04122BOX | Bolt® 4 – 12% Bis-Tris Plus Gel, 12 Well | 10 gels |
| BG04125BOX | Bolt® 4 – 12% Bis-Tris Plus Gel, 15 Well | 10 gels | NW04125BOX | Bolt® 4 – 12% Bis-Tris Plus Gel, 15 Well | 10 gels |
| BG04127BOX | Bolt® 4 – 12% Bis-Tris Plus Gel, 17 Well | 10 gels | NW04127BOX | Bolt® 4 – 12% Bis-Tris Plus Gel, 17 Well | 10 gels |
| BT2010 | Bolt® Empty Gel Cassettes | 20 cassettes | NW2010 | Bolt® Empty Mini Gel Cassettes | 20 cassettes |
| BT3010 | Bolt® Empty Gel Cassette Combs, 10 Well | 20 combs | NW3010 | Bolt® Empty Mini Gel Cassette Combs, 10 Well | 20 combs |
| BT3012 | Bolt® Empty Gel Cassette Combs, 12 Well | 20 combs | NW3012 | Bolt® Empty Mini Gel Cassette Combs, 12 Well | 20 combs |
| NA | NA | NA | A26732 | Cam Handles Kit | 2 units |
For Research Use Only. Not for use in diagnostic procedures.