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Tetra Electrophoresis Cores |
High-quality protein gel electrophoresis is essential for a successful western blotting experiment. Invitrogen offers precast protein gels in four distinct chemistries for SDS-PAGE, enabling you to customize the separation based on your target protein's molecular weight. This helps ensure sharp, well-defined bands, leading to efficient transfer and enhanced detection of your protein.
Invitrogen Tetra Electrophoresis Cores allow you to run high-performance Invitrogen Precast Protein Gels in your Bio-Rad Mini-PROTEAN® Tetra Cell tank. The Tetra Electrophoresis Cores are easy to assemble and use. The set includes:
- Tetra Electrophoresis Primary Core—run up to 2 gels
- Tetra Electrophoresis Companion Core—run two additional gels when combined with the primary core
- Buffer dam—run 1 gel when used in combination with the primary core or 3 gels when used in combination with the primary and companion cores
Upgrade to high-performance protein gels:
- Fast run times—separate your proteins in 14 minutes (Bolt Bis-Tris Plus gels) with fast protocols
- WedgeWell format—high-capacity wells with double the sample loading volume and in an easy-to-load format
- Excellent protein integrity—neutral pH helps reduce protein degradation
- Wide range of molecular weight separation—optimal protein separation
- Long shelf life—up to 16 months at room temperature
Tetra performance data
Avoid losing important information about your sample and obtain sharp straight bands for high and low molecular weight proteins alike
Both Bolt Bis-Tris Plus and Novex Tris-Glycine Plus WedgeWell format mini gels outperform Bio-Rad gels producing crisp bands and straight protein lanes, as shown in Figure 1.
Conversely, proteins can be observed in the wells of Bio-Rad for lysate loads above 20 µg, and protein bands appear to be less sharp. Bolt gradient gels in combination with MES running buffer, display an exceptional resolving power, with protein bands resolved across a broader range of the gel.
Figure 1. Performance comparison of Invitrogen mini gel electrophoresis versus Bio-Rad’s TGX mini gels run with Tetra Electrophoresis Cores in Bio-Rad’s Mini-PROTEAN Tetra Cell tank. Coomassie (SimplyBlue Safe Stain) stained gels loaded with PageRuler Plus Unstained Standard and HEK293 lysate.
Different tank, same exceptional performance
Invitrogen precast mini gels show excellent separation whether the electrophoretic separation is performed with the Tetra Electrophoresis Cores in the Bio-Rad’s Mini-PROTEAN Tetra Cell or in the Invitrogen Mini Cell Tank, as shown in Figure 2.
Figure 2. Equivalent performance of NuPAGE Bis-Tris 4–12% 10W gels run with MES running buffer in the Mini gel Tank and the Tetra Electrophoresis Cores in the Bio-Rad Mini-PROTEAN Tetra tank. Coomassie (SimplyBlue Safe Stain) stained gels loaded with PageRuler Plus Unstained Standard and HEK293 lysate.
Fast run time
Tetra Electrophoresis Cores allow the adoption of rapid protocols that reduce the overall separation time of Bolt Bis-Tris Plus and Novex Tris-Glycine Plus WedgeWell format mini gels. Figure 3 shows that the impact to gel performance is negligible.
Figure 3. The rapid protocol saves 14 minutes of run time while having similar performance to the standard protocol. Coomassie (SimplyBlue Safe Stain) stained gels loaded with PageRuler Plus Unstained Standard and HEK293 lysate.
Tetra Core Set
| Tetra Core Set | |
|---|---|
| Gel specifications | Gel size: Mini (8 x 8 cm) Gel cassette: 10 x 10 cm Gel format: standard or WedgeWell Thickness: 1.0 mm or 1.5 mm |
| Capacity | Up to 4 gels |
| Advantages | Allows running Invitrogen mini gels in Bio-Rad Mini-PROTEAN Tetra Cell |
| Buffer requirements | 750 mL for 2 gels 1100 mL for 4 gels |
| Unit dimensions | 15 x 5.1 x 14.4 cm (Primary) 15 x 5.1 x 12.3 cm (Companion) |
| Material | Polycarbonate |
| Electrode wire | Platinum |
| Electrode limits | 600 VDC or 30 Watts |
| Transfer unit | - |
| Transfer capacity | - |
| Compatible power supply | PowerEase Touch systems, Bio-Rad Power Pac systems |
| Catalog number | 1658040 |
Gel chemistry selection guide
Tailor the separation to your target protein by selecting one of the four available gel chemistries.
| Bis-Tris | Tris-Glycine | Tris-Acetate | Tricine | |
|---|---|---|---|---|
| Molecular weight range | Broad (6–400 kDa) | Broad (6–400 kDa) | High (40–500 kDa) | Low (2.5–40 kDa) |
| WedgeWell format | ✓ | ✓ | ✓ | |
| Reduced protein degradation | ✓ | ✓ | ||
| Extended shelf life | ✓ | |||
| Faster Run times | ✓ |
Electrophoresis conditions for NuPAGE and Novex Mini Gels using the Invitrogen Tetra Electrophoresis
| Gel type | Voltage (constant) | Estimated run time |
|---|---|---|
| Bolt Bis-Tris Plus with MES Running Buffer | 200 V | 20 minutes |
| Bolt Bis-Tris Plus with MOPS Running Buffer | 200 V | 28 minutes |
| NuPAGE Bis-Tris with MES Running Buffer | 200 V | 25 minutes |
| NuPAGE Bis-Tris with MOPS Running Buffer | 200 V | 38 minutes |
| Novex Tris-Glycine Plus with SDS Running Buffer | 225 V | 33 minutes |
| Novex Tris-Glycine Plus with Native Running Buffer | 225 V | 90 minutes |
| NuPAGE Tris-Acetate with SDS Running Buffer | 150 V | 54 minutes |
| NuPAGE Tris-Acetate with Native Running Buffer | 125 V | 120 minutes |
| Novex Tricine with SDS Running Buffer | 150 V | 63 minutes |
| NativePAGE Bis-Tris with NativePAGE Running Buffer | 150 V | 120 minutes |
For fast run times, the recommended rapid run conditions are provided in the following table for various types of gels. To reduce the heat generated during the rapid run, always fill the tank to the 4 gel fill line even if running only 2 gels.
| Gel type | Voltage (constant) | Estimated run time |
|---|---|---|
| Bolt Bis-Tris with MES Running Buffer | 250 V | 14 minutes |
| Novex Tris-Glycine with SDS Running Buffer | 300 V | 20 minutes |
Note: Run time may vary depending on gel percentage and the power supply used for electrophoresis. For the fastest run time, equilibrate gels to room temperature if stored at 4°C. Use caution when discarding the running buffer after the run is complete as rapid protocols will result in hotter buffer temperatures.
Power output needed
| Number of mini gels | 100 W | 200 W | 300 W |
|---|---|---|---|
| Up to 4 | ✓ | ✓ | ✓ |
| Up to 8 | X | ✓ | ✓ |
| Up to 12 | X | ✓ | ✓ |
| Up to 16 | X | X | ✓ |
Tetra performance data
Avoid losing important information about your sample and obtain sharp straight bands for high and low molecular weight proteins alike
Both Bolt Bis-Tris Plus and Novex Tris-Glycine Plus WedgeWell format mini gels outperform Bio-Rad gels producing crisp bands and straight protein lanes, as shown in Figure 1.
Conversely, proteins can be observed in the wells of Bio-Rad for lysate loads above 20 µg, and protein bands appear to be less sharp. Bolt gradient gels in combination with MES running buffer, display an exceptional resolving power, with protein bands resolved across a broader range of the gel.
Figure 1. Performance comparison of Invitrogen mini gel electrophoresis versus Bio-Rad’s TGX mini gels run with Tetra Electrophoresis Cores in Bio-Rad’s Mini-PROTEAN Tetra Cell tank. Coomassie (SimplyBlue Safe Stain) stained gels loaded with PageRuler Plus Unstained Standard and HEK293 lysate.
Different tank, same exceptional performance
Invitrogen precast mini gels show excellent separation whether the electrophoretic separation is performed with the Tetra Electrophoresis Cores in the Bio-Rad’s Mini-PROTEAN Tetra Cell or in the Invitrogen Mini Cell Tank, as shown in Figure 2.
Figure 2. Equivalent performance of NuPAGE Bis-Tris 4–12% 10W gels run with MES running buffer in the Mini gel Tank and the Tetra Electrophoresis Cores in the Bio-Rad Mini-PROTEAN Tetra tank. Coomassie (SimplyBlue Safe Stain) stained gels loaded with PageRuler Plus Unstained Standard and HEK293 lysate.
Fast run time
Tetra Electrophoresis Cores allow the adoption of rapid protocols that reduce the overall separation time of Bolt Bis-Tris Plus and Novex Tris-Glycine Plus WedgeWell format mini gels. Figure 3 shows that the impact to gel performance is negligible.
Figure 3. The rapid protocol saves 14 minutes of run time while having similar performance to the standard protocol. Coomassie (SimplyBlue Safe Stain) stained gels loaded with PageRuler Plus Unstained Standard and HEK293 lysate.
Tetra Core Set
| Tetra Core Set | |
|---|---|
| Gel specifications | Gel size: Mini (8 x 8 cm) Gel cassette: 10 x 10 cm Gel format: standard or WedgeWell Thickness: 1.0 mm or 1.5 mm |
| Capacity | Up to 4 gels |
| Advantages | Allows running Invitrogen mini gels in Bio-Rad Mini-PROTEAN Tetra Cell |
| Buffer requirements | 750 mL for 2 gels 1100 mL for 4 gels |
| Unit dimensions | 15 x 5.1 x 14.4 cm (Primary) 15 x 5.1 x 12.3 cm (Companion) |
| Material | Polycarbonate |
| Electrode wire | Platinum |
| Electrode limits | 600 VDC or 30 Watts |
| Transfer unit | - |
| Transfer capacity | - |
| Compatible power supply | PowerEase Touch systems, Bio-Rad Power Pac systems |
| Catalog number | 1658040 |
Gel chemistry selection guide
Tailor the separation to your target protein by selecting one of the four available gel chemistries.
| Bis-Tris | Tris-Glycine | Tris-Acetate | Tricine | |
|---|---|---|---|---|
| Molecular weight range | Broad (6–400 kDa) | Broad (6–400 kDa) | High (40–500 kDa) | Low (2.5–40 kDa) |
| WedgeWell format | ✓ | ✓ | ✓ | |
| Reduced protein degradation | ✓ | ✓ | ||
| Extended shelf life | ✓ | |||
| Faster Run times | ✓ |
Electrophoresis conditions for NuPAGE and Novex Mini Gels using the Invitrogen Tetra Electrophoresis
| Gel type | Voltage (constant) | Estimated run time |
|---|---|---|
| Bolt Bis-Tris Plus with MES Running Buffer | 200 V | 20 minutes |
| Bolt Bis-Tris Plus with MOPS Running Buffer | 200 V | 28 minutes |
| NuPAGE Bis-Tris with MES Running Buffer | 200 V | 25 minutes |
| NuPAGE Bis-Tris with MOPS Running Buffer | 200 V | 38 minutes |
| Novex Tris-Glycine Plus with SDS Running Buffer | 225 V | 33 minutes |
| Novex Tris-Glycine Plus with Native Running Buffer | 225 V | 90 minutes |
| NuPAGE Tris-Acetate with SDS Running Buffer | 150 V | 54 minutes |
| NuPAGE Tris-Acetate with Native Running Buffer | 125 V | 120 minutes |
| Novex Tricine with SDS Running Buffer | 150 V | 63 minutes |
| NativePAGE Bis-Tris with NativePAGE Running Buffer | 150 V | 120 minutes |
For fast run times, the recommended rapid run conditions are provided in the following table for various types of gels. To reduce the heat generated during the rapid run, always fill the tank to the 4 gel fill line even if running only 2 gels.
| Gel type | Voltage (constant) | Estimated run time |
|---|---|---|
| Bolt Bis-Tris with MES Running Buffer | 250 V | 14 minutes |
| Novex Tris-Glycine with SDS Running Buffer | 300 V | 20 minutes |
Note: Run time may vary depending on gel percentage and the power supply used for electrophoresis. For the fastest run time, equilibrate gels to room temperature if stored at 4°C. Use caution when discarding the running buffer after the run is complete as rapid protocols will result in hotter buffer temperatures.
Power output needed
| Number of mini gels | 100 W | 200 W | 300 W |
|---|---|---|---|
| Up to 4 | ✓ | ✓ | ✓ |
| Up to 8 | X | ✓ | ✓ |
| Up to 12 | X | ✓ | ✓ |
| Up to 16 | X | X | ✓ |
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