XCell SureLock Mini-Cell Electrophoresis System

The XCell SureLock Mini-Cell’s unique design allows you to run mini-gels quickly, easily, and leak-free without any clamps. The set-up time is only 30 seconds; just drop the Buffer Core and the Gel Tension Wedge into the Lower Buffer Chamber, insert the gels, and pull the Gel Tension Wedge forward. Tough polycarbonate construction of the unit boosts durability.

Power supplies  Protein gels

XCell SureLock Mini-Cell specifications

CapacityUp to 2 mini gels
Gel compatibilityGel size: 8 x 8cm
Gel cassette: 10 x 10cm
Thickness: 1.0mm or 1.5mm
Compatible gelsAll Invitrogen precast mini gels, SureCast handcast gels, and Invitrogen empty mini gel cassettes
Buffer chamber RequirementUpper chamber: 200 mL
Lower chamber: 600 mL
Optional transfer apparatusXCell II Blot Module
Transfer capacityUp to 2 mini gels
Compatible power suppliesPowerEase 90W, PowerEase 300W, Owl systems, for other systems such as Hoefer or Bio-Rad use with Novex Power Supply Adapters (Cat. No. ZA10001)
Unit dimensions14 x 13 x 16 cm (height with lid on)
MaterialPolycarbonate
Electrode wirePlatinum
Electrode limits1,500 VDC or 75 Watts
Chemical resistanceImpervious to most alcohols but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene), or acetone.
Catalog numberEI0001

The XCell SureLock Mini-Cell is covered under U.S. Patent No. 6,001,223.

How XCell SureLock Mini-Cell works

The XCell SureLock Mini-Cell holds gels firmly with a simple gel tension wedge. When the lever on the gel tension wedge is pushed forward into the locked position, an even, horizontal force is generated. This seals the gel/buffer core assembly firmly into position in the lower buffer chamber. The positive locking action of the gel tension wedge ensures a trouble-free, leak-free gel run every time.

XCell SureLock Mini-Cell Locked
Locked Position
XCell SureLock Mini-Cell Unlocked
Unlocked Position

Transfer using the XCell II Blot Module

The XCell II Blot Module allows you to easily transfer proteins or nucleic acids from mini-gels to membranes. It fits neatly into the XCell SureLock and XCell II Mini-Cells in place of the gel/buffer core assembly. It requires less than 200 mL of transfer buffer for western, Southern, and northern transfers. Tough platinized titanium and stainless steel electrodes create a uniform electrical field without clamps or hinged gel holders. Maximum blot size is 9cm x 9cm. Learn more here.

Easily set up the XCell SureLock Mini-Cell in three simple steps

XCell-SureLock-Mini-Cell-three-simple-steps-step1-155x153

Step 1. Place one or two precast gels on buffer core.

XCell-SureLock-Mini-Cell-three-simple-steps-step2-155x153

Step 2. Drop buffer core and gels into the lower buffer chamber of XCell SureLock Mini-Cell.

XCell-SureLock-Mini-Cell-three-simple-steps-step3-155x153

Step 3. Lock the gel tension wedge, load samples, fill buffer chambers, and you're set up.

Recommended electrophoresis run conditions

Gel TypeVoltage (V)Current (mA)Ending CurrentRun Time
Tris-glycine SDS-PAGE125 30-408-1290 min
Tris-glycine Native1256-123-61-12 hr
Bis-Tris (MES)200110-12570-8035-50 min
Bis-Tris (MOPS)200110-12570-8035-50 min
Tris-acetate SDS-PAGE15040-5525-4050 min
Tricine125804090 min
Zymogram12530-408-1290 min
IEF pH 3-7 or pH 3-10100-1 h
200-2 h
500- 30 min
562.5 hr
TBE20010-184-630-90 min
TBE-Urea180V10-206-1450-75 min
DNA retardation100V12-156-1590 min

XCell SureLock Mini-Cell specifications

CapacityUp to 2 mini gels
Gel compatibilityGel size: 8 x 8cm
Gel cassette: 10 x 10cm
Thickness: 1.0mm or 1.5mm
Compatible gelsAll Invitrogen precast mini gels, SureCast handcast gels, and Invitrogen empty mini gel cassettes
Buffer chamber RequirementUpper chamber: 200 mL
Lower chamber: 600 mL
Optional transfer apparatusXCell II Blot Module
Transfer capacityUp to 2 mini gels
Compatible power suppliesPowerEase 90W, PowerEase 300W, Owl systems, for other systems such as Hoefer or Bio-Rad use with Novex Power Supply Adapters (Cat. No. ZA10001)
Unit dimensions14 x 13 x 16 cm (height with lid on)
MaterialPolycarbonate
Electrode wirePlatinum
Electrode limits1,500 VDC or 75 Watts
Chemical resistanceImpervious to most alcohols but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene), or acetone.
Catalog numberEI0001

The XCell SureLock Mini-Cell is covered under U.S. Patent No. 6,001,223.

How XCell SureLock Mini-Cell works

The XCell SureLock Mini-Cell holds gels firmly with a simple gel tension wedge. When the lever on the gel tension wedge is pushed forward into the locked position, an even, horizontal force is generated. This seals the gel/buffer core assembly firmly into position in the lower buffer chamber. The positive locking action of the gel tension wedge ensures a trouble-free, leak-free gel run every time.

XCell SureLock Mini-Cell Locked
Locked Position
XCell SureLock Mini-Cell Unlocked
Unlocked Position

Transfer using the XCell II Blot Module

The XCell II Blot Module allows you to easily transfer proteins or nucleic acids from mini-gels to membranes. It fits neatly into the XCell SureLock and XCell II Mini-Cells in place of the gel/buffer core assembly. It requires less than 200 mL of transfer buffer for western, Southern, and northern transfers. Tough platinized titanium and stainless steel electrodes create a uniform electrical field without clamps or hinged gel holders. Maximum blot size is 9cm x 9cm. Learn more here.

Easily set up the XCell SureLock Mini-Cell in three simple steps

XCell-SureLock-Mini-Cell-three-simple-steps-step1-155x153

Step 1. Place one or two precast gels on buffer core.

XCell-SureLock-Mini-Cell-three-simple-steps-step2-155x153

Step 2. Drop buffer core and gels into the lower buffer chamber of XCell SureLock Mini-Cell.

XCell-SureLock-Mini-Cell-three-simple-steps-step3-155x153

Step 3. Lock the gel tension wedge, load samples, fill buffer chambers, and you're set up.

Recommended electrophoresis run conditions

Gel TypeVoltage (V)Current (mA)Ending CurrentRun Time
Tris-glycine SDS-PAGE125 30-408-1290 min
Tris-glycine Native1256-123-61-12 hr
Bis-Tris (MES)200110-12570-8035-50 min
Bis-Tris (MOPS)200110-12570-8035-50 min
Tris-acetate SDS-PAGE15040-5525-4050 min
Tricine125804090 min
Zymogram12530-408-1290 min
IEF pH 3-7 or pH 3-10100-1 h
200-2 h
500- 30 min
562.5 hr
TBE20010-184-630-90 min
TBE-Urea180V10-206-1450-75 min
DNA retardation100V12-156-1590 min
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