XCell SureLock Mini-Cell Electrophoresis System

The XCell SureLock Mini-Cell’s unique design allows you to run mini-gels quickly, easily, and leak-free without any clamps. The set-up time is only 30 seconds; just drop the Buffer Core and the Gel Tension Wedge into the Lower Buffer Chamber, insert the gels, and pull the Gel Tension Wedge forward. Tough polycarbonate construction of the unit boosts durability.

Power supplies  Protein gels

XCell SureLock Mini-Cell specifications

Capacity Up to 2 mini gels
Gel compatibility Gel size: 8 x 8cm
Gel cassette: 10 x 10cm
Thickness: 1.0mm or 1.5mm
Compatible gels All Invitrogen precast mini gels, SureCast handcast gels, and Invitrogen empty mini gel cassettes
Buffer chamber Requirement Upper chamber: 200 mL
Lower chamber: 600 mL
Optional transfer apparatus XCell II Blot Module
Transfer capacity Up to 2 mini gels
Compatible power supplies PowerEase 90W, PowerEase 300W, Owl systems, for other systems such as Hoefer or Bio-Rad use with Novex Power Supply Adapters (Cat. No. ZA10001)
Unit dimensions 14 x 13 x 16 cm (height with lid on)
Material Polycarbonate
Electrode wire Platinum
Electrode limits 1,500 VDC or 75 Watts
Chemical resistance Impervious to most alcohols but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene), or acetone.
Catalog number EI0001

The XCell SureLock Mini-Cell is covered under U.S. Patent No. 6,001,223.

How XCell SureLock Mini-Cell works

The XCell SureLock Mini-Cell holds gels firmly with a simple gel tension wedge. When the lever on the gel tension wedge is pushed forward into the locked position, an even, horizontal force is generated. This seals the gel/buffer core assembly firmly into position in the lower buffer chamber. The positive locking action of the gel tension wedge ensures a trouble-free, leak-free gel run every time.

XCell SureLock Mini-Cell Locked
Locked Position
XCell SureLock Mini-Cell Unlocked
Unlocked Position

Transfer using the XCell II Blot Module

The XCell II Blot Module allows you to easily transfer proteins or nucleic acids from mini-gels to membranes. It fits neatly into the XCell SureLock and XCell II Mini-Cells in place of the gel/buffer core assembly. It requires less than 200 mL of transfer buffer for western, Southern, and northern transfers. Tough platinized titanium and stainless steel electrodes create a uniform electrical field without clamps or hinged gel holders. Maximum blot size is 9cm x 9cm. Learn more here.

Easily set up the XCell SureLock Mini-Cell in three simple steps

XCell-SureLock-Mini-Cell-three-simple-steps-step1-155x153

Step 1. Place one or two precast gels on buffer core.

XCell-SureLock-Mini-Cell-three-simple-steps-step2-155x153

Step 2. Drop buffer core and gels into the lower buffer chamber of XCell SureLock Mini-Cell.

XCell-SureLock-Mini-Cell-three-simple-steps-step3-155x153

Step 3. Lock the gel tension wedge, load samples, fill buffer chambers, and you're set up.

Recommended electrophoresis run conditions

Gel Type Voltage (V) Current (mA) Ending Current Run Time
Tris-glycine SDS-PAGE 125  30-40 8-12 90 min
Tris-glycine Native 125 6-12 3-6 1-12 hr
Bis-Tris (MES) 200 110-125 70-80 35-50 min
Bis-Tris (MOPS) 200 110-125 70-80 35-50 min
Tris-acetate SDS-PAGE 150 40-55 25-40 50 min
Tricine 125 80 40 90 min
Zymogram 125 30-40 8-12 90 min
IEF pH 3-7 or pH 3-10 100-1 h
200-2 h
500- 30 min
5 6 2.5 hr
TBE 200 10-18 4-6 30-90 min
TBE-Urea 180V 10-20 6-14 50-75 min
DNA retardation 100V 12-15 6-15 90 min

XCell SureLock Mini-Cell specifications

Capacity Up to 2 mini gels
Gel compatibility Gel size: 8 x 8cm
Gel cassette: 10 x 10cm
Thickness: 1.0mm or 1.5mm
Compatible gels All Invitrogen precast mini gels, SureCast handcast gels, and Invitrogen empty mini gel cassettes
Buffer chamber Requirement Upper chamber: 200 mL
Lower chamber: 600 mL
Optional transfer apparatus XCell II Blot Module
Transfer capacity Up to 2 mini gels
Compatible power supplies PowerEase 90W, PowerEase 300W, Owl systems, for other systems such as Hoefer or Bio-Rad use with Novex Power Supply Adapters (Cat. No. ZA10001)
Unit dimensions 14 x 13 x 16 cm (height with lid on)
Material Polycarbonate
Electrode wire Platinum
Electrode limits 1,500 VDC or 75 Watts
Chemical resistance Impervious to most alcohols but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene), or acetone.
Catalog number EI0001

The XCell SureLock Mini-Cell is covered under U.S. Patent No. 6,001,223.

How XCell SureLock Mini-Cell works

The XCell SureLock Mini-Cell holds gels firmly with a simple gel tension wedge. When the lever on the gel tension wedge is pushed forward into the locked position, an even, horizontal force is generated. This seals the gel/buffer core assembly firmly into position in the lower buffer chamber. The positive locking action of the gel tension wedge ensures a trouble-free, leak-free gel run every time.

XCell SureLock Mini-Cell Locked
Locked Position
XCell SureLock Mini-Cell Unlocked
Unlocked Position

Transfer using the XCell II Blot Module

The XCell II Blot Module allows you to easily transfer proteins or nucleic acids from mini-gels to membranes. It fits neatly into the XCell SureLock and XCell II Mini-Cells in place of the gel/buffer core assembly. It requires less than 200 mL of transfer buffer for western, Southern, and northern transfers. Tough platinized titanium and stainless steel electrodes create a uniform electrical field without clamps or hinged gel holders. Maximum blot size is 9cm x 9cm. Learn more here.

Easily set up the XCell SureLock Mini-Cell in three simple steps

XCell-SureLock-Mini-Cell-three-simple-steps-step1-155x153

Step 1. Place one or two precast gels on buffer core.

XCell-SureLock-Mini-Cell-three-simple-steps-step2-155x153

Step 2. Drop buffer core and gels into the lower buffer chamber of XCell SureLock Mini-Cell.

XCell-SureLock-Mini-Cell-three-simple-steps-step3-155x153

Step 3. Lock the gel tension wedge, load samples, fill buffer chambers, and you're set up.

Recommended electrophoresis run conditions

Gel Type Voltage (V) Current (mA) Ending Current Run Time
Tris-glycine SDS-PAGE 125  30-40 8-12 90 min
Tris-glycine Native 125 6-12 3-6 1-12 hr
Bis-Tris (MES) 200 110-125 70-80 35-50 min
Bis-Tris (MOPS) 200 110-125 70-80 35-50 min
Tris-acetate SDS-PAGE 150 40-55 25-40 50 min
Tricine 125 80 40 90 min
Zymogram 125 30-40 8-12 90 min
IEF pH 3-7 or pH 3-10 100-1 h
200-2 h
500- 30 min
5 6 2.5 hr
TBE 200 10-18 4-6 30-90 min
TBE-Urea 180V 10-20 6-14 50-75 min
DNA retardation 100V 12-15 6-15 90 min
Resources

Download Protein Gel Electrophoresis Technical Handbook

Download the Protein Gel Chemistry Guide

Download Complete, innovative western workflow solutions brochure