XCell4 SureLock Midi Cell electrophoresis chamber system

The XCell4 SureLock Midi-Cell Electrophoresis System's unique design allows you to quickly and easily perform simultaneous vertical electrophoresis of 1-4 midi-gels with consistent performance. The XCell4 SureLock Midi-Cell is built with tough polycarbonate to boost durability and features a specially designed lid to enhance user safety.

Power supplies  Protein gels

XCell4 SureLock Midi-Cell specifications

Capacity Up to 4 midi gels
Gel compatibility Gel size: 8 x 13cm
Gel cassette: 10.3 x 15cm
Thickness: 1.0mm
Compatible gels All Invitrogen precast midi gels, and Invitrogen empty midi gel cassettes
Buffer chamber Requirement Upper chamber: 175 mL (per gel)
Lower chamber: 700 mL (with 4 gels)
Compatible power supplies PowerEase 300, Zoom Dual Power, Owl systems, for other systems such as Hoefer or Bio-Rad use with Novex Power Supply Adapters (Cat. No. ZA10001)
Unit dimensions 21 x 19 x 16 cm (height with lid on)
Material Polycarbonate
Electrode wire Platinum
Electrode limits 600 VDC or 200 Watts
Chemical resistance Impervious to most alcohols but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene), or acetone.
Catalog number WR0100

XCell4 SureLock Midi-Cell system overview

The XCell4 SureLock Midi-Cell Electrophoresis System consists of a Lower Buffer Chamber, two Buffer Cores, Buffer Dam, Displacement Dam, and a lid with cables.

Electrophoresis of proteins on midi-gels in the XCell4 SureLock Midi-Cell is performed after assembling each Buffer Core with 2 midi gels or a Buffer Core with a midi-gel and a Buffer Dam. The Buffer Core/gel assembly is inserted into the Lower Buffer Chamber and is sealed using a positive locking mechanism that seals the Buffer Core/gel assembly firmly into position and ensures leak-free electrophoresis.

The Buffer Core/gel assembly creates separate upper buffer chambers for each gel and a common lower buffer chamber shared by all gels. The lid is placed onto the Midi-Cell and fits on the Midi-Cell in only one orientation to prevent incorrect connection to the electrodes. The unique design of the XCell4 SureLock Midi-Cell evenly dissipates heat resulting in consistent performance of all gels without the need for any external cooling device. The Buffer Core electrical poles are color-coded for easy, intuitive assembly.

Key features of the XCell4 SureLock Midi-Cell Electrophoresis System:

  • User-friendly design without any clamps for leak-free electrophoresis
  • Flexibility to perform electrophoresis of 1-4 midi-gels
  • Unique, heat-dissipating design of the XCell4 SureLock Midi-Cell produces consistent results without the need for any cooling device
  • Built-in safety features to enhance user safety
Schematic of Xcell4 SureLock Mid-Cell Electrophoresis chamber

Schematic of XCell4 SureLock Midi-Cell Electrophoresis Unit.

Recommended run conditions

Gel Type Voltage (V) Current* (mA) Ending Current* Run Time
Tris-glycine SDS-PAGE 125  40-50 20-25 105 min
Tris-glycine Native 125 35-40 15-20 1-12 hr
Bis-Tris (MES) 200 160-200 120-170 40 min
Bis-Tris (MOPS) 200 160-200 120-170 55 min
Tris-acetate SDS-PAGE 150 70-90 50-60 70 min
Tris-acetate Native 150 40-45 15-20 2-3 hr

*Current readings are per gel

If simultaneously running 4 gels using the XCell4 SureLock Midi-Cell, we recommend using the ZOOM Dual Power Supply or PowerEase 300. The Low Voltage/High Current outlets can handle the voltage and current of four Bis-Tris gels (Voltage 200 V, Current ~800 mA). The PowerEase 90W Power Supply can be used with the XCell4 SureLock Midi-Cell but the current limit of 500 mA will be reached with 2 gels (2 x ~200 mA max current at 200V). If 4 gels are run in the XCell4 SureLock Midi-Cell with these power supplies, the current will be maxed out and the run will slow down. It will take approximately twice as long to run the gels. Note: To allow connection of the covered power leads of the XCell4 SureLock Midi-Cell with certain power supplies that are not designed for use with covered or non-retractable power leads, use Invitrogen Power Supply Adapters available separately (Cat. No. ZA10001).

XCell4 SureLock Midi-Cell specifications

Capacity Up to 4 midi gels
Gel compatibility Gel size: 8 x 13cm
Gel cassette: 10.3 x 15cm
Thickness: 1.0mm
Compatible gels All Invitrogen precast midi gels, and Invitrogen empty midi gel cassettes
Buffer chamber Requirement Upper chamber: 175 mL (per gel)
Lower chamber: 700 mL (with 4 gels)
Compatible power supplies PowerEase 300, Zoom Dual Power, Owl systems, for other systems such as Hoefer or Bio-Rad use with Novex Power Supply Adapters (Cat. No. ZA10001)
Unit dimensions 21 x 19 x 16 cm (height with lid on)
Material Polycarbonate
Electrode wire Platinum
Electrode limits 600 VDC or 200 Watts
Chemical resistance Impervious to most alcohols but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene), or acetone.
Catalog number WR0100

XCell4 SureLock Midi-Cell system overview

The XCell4 SureLock Midi-Cell Electrophoresis System consists of a Lower Buffer Chamber, two Buffer Cores, Buffer Dam, Displacement Dam, and a lid with cables.

Electrophoresis of proteins on midi-gels in the XCell4 SureLock Midi-Cell is performed after assembling each Buffer Core with 2 midi gels or a Buffer Core with a midi-gel and a Buffer Dam. The Buffer Core/gel assembly is inserted into the Lower Buffer Chamber and is sealed using a positive locking mechanism that seals the Buffer Core/gel assembly firmly into position and ensures leak-free electrophoresis.

The Buffer Core/gel assembly creates separate upper buffer chambers for each gel and a common lower buffer chamber shared by all gels. The lid is placed onto the Midi-Cell and fits on the Midi-Cell in only one orientation to prevent incorrect connection to the electrodes. The unique design of the XCell4 SureLock Midi-Cell evenly dissipates heat resulting in consistent performance of all gels without the need for any external cooling device. The Buffer Core electrical poles are color-coded for easy, intuitive assembly.

Key features of the XCell4 SureLock Midi-Cell Electrophoresis System:

  • User-friendly design without any clamps for leak-free electrophoresis
  • Flexibility to perform electrophoresis of 1-4 midi-gels
  • Unique, heat-dissipating design of the XCell4 SureLock Midi-Cell produces consistent results without the need for any cooling device
  • Built-in safety features to enhance user safety
Schematic of Xcell4 SureLock Mid-Cell Electrophoresis chamber

Schematic of XCell4 SureLock Midi-Cell Electrophoresis Unit.

Recommended run conditions

Gel Type Voltage (V) Current* (mA) Ending Current* Run Time
Tris-glycine SDS-PAGE 125  40-50 20-25 105 min
Tris-glycine Native 125 35-40 15-20 1-12 hr
Bis-Tris (MES) 200 160-200 120-170 40 min
Bis-Tris (MOPS) 200 160-200 120-170 55 min
Tris-acetate SDS-PAGE 150 70-90 50-60 70 min
Tris-acetate Native 150 40-45 15-20 2-3 hr

*Current readings are per gel

If simultaneously running 4 gels using the XCell4 SureLock Midi-Cell, we recommend using the ZOOM Dual Power Supply or PowerEase 300. The Low Voltage/High Current outlets can handle the voltage and current of four Bis-Tris gels (Voltage 200 V, Current ~800 mA). The PowerEase 90W Power Supply can be used with the XCell4 SureLock Midi-Cell but the current limit of 500 mA will be reached with 2 gels (2 x ~200 mA max current at 200V). If 4 gels are run in the XCell4 SureLock Midi-Cell with these power supplies, the current will be maxed out and the run will slow down. It will take approximately twice as long to run the gels. Note: To allow connection of the covered power leads of the XCell4 SureLock Midi-Cell with certain power supplies that are not designed for use with covered or non-retractable power leads, use Invitrogen Power Supply Adapters available separately (Cat. No. ZA10001).

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