Protein Gel Staining and Imaging
Whether you just need a quick visual confirmation or require a highly-sensitive stain to detect low-abundance proteins, we offer a variety of easy-to-use, effective protein stains for both in-gel and on-membrane detection. Check out our comprehensive collection of stains including Coomassie dye-based, silver, fluorescent, tagged fusion protein, specialty, and multiplexed proteomics protein gel stains.
Choose the protein staining method most suitable for your experiments
Coomassie Staining | Silver Staining | Fluorescent Protein Staining | |
---|---|---|---|
Sensitivity |
25 ng |
0.5 ng |
0.5 ng |
Ease of use |
+++ |
+ |
+ |
Mode of action |
In acidic buffer conditions, coomassie dye binds to basic and hydrophobic residues of proteins, changing from dull reddish-brown to intense blue. |
Silver ions interact and bind with with carboxylic acid groups ( Asp and Glu), imidazole (His), sulfhydryls (Cys), and amines (Lys). Silver ions are reduced to metallic silver, resulting in brown-black color. |
Most fluorescent stains involve simply dye-binding mechanisms rather than chemical reactions that alter protein functional groups. |
Detection |
Visual |
Visual |
Compatible imaging system |
Compatibiltity with downstream applications |
MS and sequencing compatible |
Certain formulations are MS compatible |
Most stains are MS compatible |
Learn more | Learn more | Learn more |
Featured additional staining solutions
Preferentially detect histidine-tagged (His-tagged) recombinant fusion proteins in polyacrylamide gels
Includes in-gel phospho- and glycoprotein detection and on-membrane reversible protein staining kits
For Research Use Only. Not for use in diagnostic procedures.