Easy-to-use Coomassie™ dye-based protein stains for visualization of bands resolved by protein gel electrophoresis (SDS-PAGE).

The most common method for in-gel protein detection is staining with coomassie dye. Several Coomassie™ stain reagent recipes exist in the literature and use either the G-250 (“colloidal”) or R-250 form of the dye. Colloidal Coomassie™ can be formulated to effectively stain proteins within one hour and require only water (no methanol or acetic acid) for destaining.

Coomassie™ dye staining is especially convenient because it involves a single, ready-to-use reagent and does not permanently chemically modify the target proteins. Because no chemical modification occurs, excised protein bands can be completely destained and the proteins recovered for analysis by mass spectrometry or sequencing.


  • Simple—Coomassie™ dye-based formulations are easy to formulate and are widely used
  • Easy-to-use—simply soak the gel in the stain solution and destain to observe protein bands
  • Economical—Coomassie™ dye-based stain formulations are cost effective
  • Compatible—useful for qualitative visualization, quantitative densitometry, and downstream excision and analysis by mass spectrometry

Select the Coomassie dye–based stain that is right for your needs

  Simply Blue™ Safe Stain Imperial Protein Stain PageBlue Protein Stain
Components (steps) 1 (5) 1 (5) 1 (5)
Limit of detection >7 ng 3 ng 5 ng
Mass spec. compatible Yes Yes Yes
Advantage(s) Rapid sensitive staining without hazardous shipping costs Fast, ultrasensitive protein detection Cost-effective option for fast, sensitive staining
  Order now Order now Order now
*The number of steps includes multiple gel washes, solution changes and destaining steps.

SimplyBlue™ SafeStain is a ready-to-use, fast, sensitive, and safe Coomassie® G-250 stain for visualizing protein bands on polyacrylamide gels. This stain eliminates extensive solution preparation time and expenditure. Unlike traditional R-250 and some G-250 Coomassie™ stains, SimplyBlue™ SafeStain is completely non-hazardous and does not require methanol or acetic acid fixatives or destains. Risk of hazardous exposure and unpleasant odors are eliminated, creating a healthier lab environment.


The SimplyBlue™ SafeStain protocol is easy to perform and can be completed in less than 3 hours (see figure). For added speed, a simple microwave procedure can be completed in 12 minutes. Destaining is not required, but may be performed to achieve maximum sensitivity, especially when performing downstream analysis such as mass spectrometry or when a crystal-clear background is required.



One microgram of protein can be detected after just 5 minutes in the stain. With additional water-based destaining, as little as 7 ng of protein (reduced BSA) can be detected (see figure).


Thermo Scientific Imperial Protein Stain is a ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers consistent nanogram-level detection of proteins in polyacrylamide electrophoresis gels.

Imperial Protein Stain is a coomassie dye reagent for detection of protein bands in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and 2D gels. The stain is a unique formulation of coomassie brilliant blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercial stains. Staining results in intensely colored protein bands that are easy to photograph and document with gel imagers


The Imperial Protein Stain protocol is easy to perform and takes less than 2 hours to complete. Detect down to 6ng protein per band in just 20 mins. Additionally, the protocol uses simple water-wash steps rather than methanol/acetic acid fixation and destaining, which saves valuable preparation time and minimizes reagent costs.


Thermo Scientific Imeprial Protein Stain is one of the most sensitive colorimetric stains available, easily detecting 3 to 6 nanograms of protein per band.(see figure below)


Thermo Scientific PageBlue Protein Staining Solution is a sensitive, economical formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid.


The simple PageBlue Protein Staining Solution protocol is sensitive, efficient and reduces the concern of over staining gels even after overnight staining. The protocol is fast with the microwave procedure taking about 30 mins and the standard procedure taking about 90 mins to perform.


This protein stain is designed to deliver a dynamic range of 5 to 500 ng, which is approximately 10 times more sensitive than traditional coomassie R-250-based dyes. Furthermore, PageBlue Protein Staining Solution is economical because it can be reused up to three times (within a 1-year period) without any loss in sensitivity.