Thermo Scientific Pierce™ Silver Stain for Mass Spectrometry is a complete kit for the rapid and sensitive silver staining of proteins in polyacrylamide gels and the efficient destaining of excised gel pieces for mass spectrometry analysis.
This silver staining kit for mass spectrometry (MS) bundles components of the high-performance Pierce™ Silver Stain Kit with reagents optimized to destain spots for subsequent in-gel tryptic digestion to recover peptide fragments for proteomics analysis. The resulting MS-compatible product and protocol deliver outstanding sensitivity and help maintain favorable conditions for high-yield recovery and identification (sequence coverage) of proteins by mass spectrometry.
Silver staining of 2D gels is an important intermediate step in a set of procedures that leads to identification of specific proteins in the proteome by mass fingerprinting methods. The Pierce™ Silver Stain protocol provides, flexibility, reliability, and robustness for applications such as MALDI-MS.