Our Specialty Protein Staining Kits include in gel phospho- and glycoprotein detection and on-membrane reversible protein staining kits.

Choose the staining kit that is right for your experiment

 

Pro-Q® Emerald 488 Glycoprotein Gel and Blot Stain kit

Pro-Q® Emerald 300 Glycoprotein Gel and Blot Stain kit

Pro-Q® Diamond Phosphoprotein Gel Staining Kit

Multiplexed Proteomics Phosphoprotein Gel Stain Kit

Pierce™ Reversible Protein Stain Kits for Membranes

For staining

Glycoproteins

Glycoproteins

Phosphoproteins

Phosphoproteins and total protein

Total protein

Sensitivity

4 ng glycoprotein/band

0.5 ng glycoprotein/
band

1–16 ng phosphoprotein/
band

1–16 ng phosphoprotein and 0.25 ng total protein/band

25–50 ng

Stain/destain

About 6 hr

About 5 hr

4–5 hr

4–5 hr

20 min

Ex/Em

510/520 nm

280/530 nm

555/580 nm

555/580 nm
450/610 nm

NA

Advantages

Selective staining of glycoproteins

Selective staining of glycoproteins

Selective staining of phosphoproteins

For quantitative proteome analysis

For protein detection on nitrocellulose or PVDF membrane after transfer from polyacrylamide gels

Order now Order now Order now Learn more Order now

Pro-Q® Emerald Glycoprotein Gel and Blot Stain kits

The Pro-Q® Emerald 300 and Pro-Q® Emerald 488 Glycoprotein Stain Kits provide highly advanced technology for detection of glycoproteins in gels and on blots. Gel staining is rapid and very sensitive. Typically, in less than three hours, it is possible to detect as little as 300 pg of glycoprotein per band, depending on the degree of glycosylation, making these stains at least 50-fold more sensitive than standard fuchsin staining.

Pro-Q® Diamond Phosphoprotein Gel Staining Kit

The Pro-Q® Diamond Phosphoprotein Gel Staining Kit, includes 1 L of the Pro-Q® Diamond Phosphoprotein Gel Stain and 40 µL of the PeppermintStick™ phosphoprotein molecular weight standards. The Pro-Q® Diamond phosphoprotein gel stain is a fluorescent stain (excitation/emission maxima ~555/580 nm) that can detect as little as 1–16 ng of phosphoprotein per band with a signal that is linear over 3 orders of magnitude. The PeppermintStick™ phosphoprotein molecular weight standards are a mixture of phosphorylated and nonphosphorylated proteins with molecular weights from 14,000 to 116,250 Da that serve both as molecular weight markers and as positive and negative controls.

Multiplexed Proteomics™ Technology for Protein Profiling Analysis
Multiplexed-Proteomics-Technology

The Molecular Probes® Multiplexed Proteomics™ Technology is designed to directly visualize differential phosphorylation patterns, compared to total protein, in the same gel. The Multiplexed Proteomics™ Kit contains Pro-Q® Diamond Phosphoprotein Stain and SYPRO® Ruby Total Protein Stain. Phosphoproteins are stained and imaged relative to total protein in the same gel.

This allows unambiguous matching of proteins bands or spots and relative quantitation of differential phosphorylation patterns. Differences in posttranslational modifications relative to total protein expression can be distinguished in complex samples by two-dimensional gel electrophoresis, or multiple sample fractions can be screened in order to locate proteins of interest.

Pierce Reversible Protein Stain Kits for Membranes

The Thermo Scientific Pierce Reversible Protein Stain Kits for Membranes are rapid and sensitive alternatives to Ponceau S stain for protein detection on nitrocellulose or PVDF membrane after transfer from polyacrylamide gels.

These kits for membrane-staining use a nondestructive, reversible, reliable and sensitive method to stain and detect proteins on nitrocellulose and PVDF membranes. These kits are superior alternatives to Ponceau S stains for evaluating the efficiency of protein transfer following SDS-PAGE and before immunoblotting. The lower limit of detection with this method is 25 to 50 ng per band (at least five times more sensitive than traditional Ponceau S staining). The staining protocols are simple, quick and result in turquoise-blue bands that do not fade and are easily photographed for future reference. The stain can be easily reversed in typically less than 15 minutes. Subsequent western blot detection is unaffected because the stain does not alter the protein and is completely removed. Separate kits for nitrocellulose and PVDF membranes use the same dye-binding mechanism but differ in the stain formulation and membrane-treatment components to optimize the speed and performance with each type of membrane.