Protein Gels

Explore our protein gel options. Choose from precast polyacrylamide gel electrophoresis (PAGE) chemistries designed for specific applications, each available in a variety of well and cassette formats, or select a system for pouring and casting your own gels.

Find the right gel for your protein

Convert from other suppliers' gels

Featured protein gel categories

  Bolt Bis-Tris Plus Gels
Bolt Bis-Tris Plus Gels
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NuPAGE Bis-Tris Gels
NuPAGE Bis-Tris Gels
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Novex Tris-Glycine Gels
Novex Tris-Glycine Gels
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NuPAGE Tris-Acetate GelsNuPAGE Tris-Acetate Gels
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Tricine GelsNovex Tricine Gels
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Gel chemistry Bis-Tris Bis-Tris Tris-Glycine Tris-Acetate Tricine
Protein sample type Broad range MW (6-400 kDa) Broad range MW (6-400 kDa) Broad range MW (6-400 kDa) High range MW (40-500 kDa) Low range MW (2.5-40 kDa)
Product benefits Neutral pH for high sensitivity applications with larger loading capacity. Load up to 60 µL per well. Run times as fast as 20-35 minutes Neutral pH for high sensitivity applications such as western blotting, mass spectrometry Traditional Laemmli-style gels with larger loading capacity- load up to 60 µL per well Neutral pH for better separation, transfer and analysis of high molecular weight proteins Better separation, transfer and analysis of low molecular weight proteins
Recommened for Western blotting, mass spectrometry, post translationally modified proteins, dilute samples, denaturing applications Western blotting, mass spectrometry, post translationally modified proteins, denaturing applications Western blotting, in-gel staining, samples containing detergent and high salt, denaturing, or native PAGE applications High molecular weight proteins, Western blotting, mass spectrometry, post translationally modified proteins, denaturing or native PAGE applications Low molecular weight proteins, western blotting, in-gel staining, denaturing applications

Bis-Tris

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
Bolt Bis-Tris Plus
  • Dilute or larger (up to 60 µL) samples 
  • Separation of small- to medium-sized proteins
  • Western blot transfer and analysis, and all techniques in which protein integrity is crucial
8%, 10%, 12%, 4–12% 6 to 400 kDa
  • Mini with larger well capacity (WedgeWell, up to 60 µL)
22–45 min up to 16 months
NuPAGE Bis-Tris
  • Separation of small- to medium-sized proteins
  • Western blot transfer and analysis, and all techniques in which protein integrity is crucial
8%, 10%, 12%, 4–12% 1.5 to 300 kDa
  • Mini
  • Midi
35–50 min 12 months

Tris-Glycine

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life

Novex Tris-Glycine

 

  • Separation of small- to medium-sized proteins using traditional Laemmli-style gels

6%, 8%, 10%, 12%,

4–20%, 4–12%, 14%,

16%, 8–16% , 10–20%

8 to 250 kDa

  • Mini with larger well capacity (WedgeWell, up to 60 µL)
  • Midi

90 min

up to 12 months

Tris-Acetate

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
NuPAGE Tris-Acetate
  • Separation of larger proteins
7%, 3–8% 36 to 500 kDa
  • Mini
  • Midi
60 min 6 months

Tricine

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
Novex Tricine
  • Separation of small proteins and peptides
10%, 16% 2 to 200 kDa
  • Mini
90 min 1–2 months

Native Bis-Tris

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life

NativePAGE Bis-Tris

  • Separation of native proteins
3–12%, 4–16% 15 to 10,000 kDa
  • Mini
90–120 min 12 months

IEF

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
IEF
  • Separation of proteins based on isoelectric point
pH 3–7, pH 3–10 pI 3.5 to pI 8.5
  • Mini
150 min 2 months

Zymogram

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
Zymogram
  • Separation of proteins based on size and activity
10% (with gelatin) 10 to 220 kDa
  • Mini
90 min 2 months

E-PAGE

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
E-PAGE
  • High throughput for recombination product analysis and protein profiling
E-PAGE 96:6%
E-PAGE 48:8%
10 to 220 kDa
  • 48-well
  • 96-well
14–25 min 6 months
  NativePAGE Bis-Tris Gels
NativePAGE Bis-Tris Gels
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Isoelectric Focusing Gels
Isoelectric Focusing Gels
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Novex Zymogram Gels
Novex Zymogram Gels
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E-PAGE Precast Gels
E-PAGE Precast
Gels

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Gel chemistry Bis-Tris pH 3–7, pH 3–10 Tris-glycine with gelatin 8% or 6%
Protein sample type 15 to 10,000 kDa pI 3.5 to pI 8.5 10 to 220 kDa 10-200 kDa
Product benefits Separation of native proteins, neutral pH for high sensitivity applications Separation of proteins based on isoelectric point Separation of proteins based on size and activity High-throughput gel system, that eliminates the need for buffers
  SureCast Handcast System
SureCast Handcast System
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Empty Gel Cassettes
Empty Gel Cassettes
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Bolt Empty Cassettes
Bolt Empty Cassettes
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Cassette type Resuable, glass Single use, pre-sealed Single use, pre-sealed
Product benefits
  • Leak-free design—minimize the number of failed gels, waste less time
  • Superior glass plate durability
  • Unique tilt feature—minimize spillage and confidently load acrylamide solutions
  • Simple component assembly—using a single-motion load-and-lock mechanism
  • Ready-to-pour cassettes, casting stand not required, cassettes sealed on three sides (no gel leakage)
  • Special coating provides uniform polymerization rate for a more consistent handcast gel
  • High capacity sample wells- load up to 60 µL per well
  • Ready-to-pour cassettes, casting stand not required, cassettes sealed on three sides (no gel leakage)
  • Special coating provides uniform polymerization rate for a more consistent handcast gel
Gel dimensions Mini: 8x8 cm (1 mm thick)

Mini: 8x8 cm (1 or 1.5 mm thick)

Midi: 8X13 cm (1 mm thick)

Mini: 8x8 cm (1 mm thick)
Gel tank Mini Gel Tank, Xcell SureLock Mini-Cell

Mini Gels: Mini Gel Tank or XCell SureLock Mini-Cell

Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only)

Mini Gel Tank, Xcell SureLock Mini-Cell
Reagents SureCast reagents, other popular polyacrylamide reagents SureCast reagents, other popular polyacrylamide reagents SureCast reagents, other popular polyacrylamide reagents
Combos available 1, 2, 10, 12, 15-wells 1, 2, 5, 10, 12, 15 and 2D-wells 10 or 12-wells (high capacity wells)
  Bolt Bis-Tris Plus Gels
Bolt Bis-Tris Plus Gels
Explore
NuPAGE Bis-Tris Gels
NuPAGE Bis-Tris Gels
Explore
Novex Tris-Glycine Gels
Novex Tris-Glycine Gels
Explore
NuPAGE Tris-Acetate GelsNuPAGE Tris-Acetate Gels
Explore
Tricine GelsNovex Tricine Gels
Explore
Gel chemistry Bis-Tris Bis-Tris Tris-Glycine Tris-Acetate Tricine
Protein sample type Broad range MW (6-400 kDa) Broad range MW (6-400 kDa) Broad range MW (6-400 kDa) High range MW (40-500 kDa) Low range MW (2.5-40 kDa)
Product benefits Neutral pH for high sensitivity applications with larger loading capacity. Load up to 60 µL per well. Run times as fast as 20-35 minutes Neutral pH for high sensitivity applications such as western blotting, mass spectrometry Traditional Laemmli-style gels with larger loading capacity- load up to 60 µL per well Neutral pH for better separation, transfer and analysis of high molecular weight proteins Better separation, transfer and analysis of low molecular weight proteins
Recommened for Western blotting, mass spectrometry, post translationally modified proteins, dilute samples, denaturing applications Western blotting, mass spectrometry, post translationally modified proteins, denaturing applications Western blotting, in-gel staining, samples containing detergent and high salt, denaturing, or native PAGE applications High molecular weight proteins, Western blotting, mass spectrometry, post translationally modified proteins, denaturing or native PAGE applications Low molecular weight proteins, western blotting, in-gel staining, denaturing applications

Bis-Tris

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
Bolt Bis-Tris Plus
  • Dilute or larger (up to 60 µL) samples 
  • Separation of small- to medium-sized proteins
  • Western blot transfer and analysis, and all techniques in which protein integrity is crucial
8%, 10%, 12%, 4–12% 6 to 400 kDa
  • Mini with larger well capacity (WedgeWell, up to 60 µL)
22–45 min up to 16 months
NuPAGE Bis-Tris
  • Separation of small- to medium-sized proteins
  • Western blot transfer and analysis, and all techniques in which protein integrity is crucial
8%, 10%, 12%, 4–12% 1.5 to 300 kDa
  • Mini
  • Midi
35–50 min 12 months

Tris-Glycine

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life

Novex Tris-Glycine

 

  • Separation of small- to medium-sized proteins using traditional Laemmli-style gels

6%, 8%, 10%, 12%,

4–20%, 4–12%, 14%,

16%, 8–16% , 10–20%

8 to 250 kDa

  • Mini with larger well capacity (WedgeWell, up to 60 µL)
  • Midi

90 min

up to 12 months

Tris-Acetate

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
NuPAGE Tris-Acetate
  • Separation of larger proteins
7%, 3–8% 36 to 500 kDa
  • Mini
  • Midi
60 min 6 months

Tricine

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
Novex Tricine
  • Separation of small proteins and peptides
10%, 16% 2 to 200 kDa
  • Mini
90 min 1–2 months

Native Bis-Tris

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life

NativePAGE Bis-Tris

  • Separation of native proteins
3–12%, 4–16% 15 to 10,000 kDa
  • Mini
90–120 min 12 months

IEF

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
IEF
  • Separation of proteins based on isoelectric point
pH 3–7, pH 3–10 pI 3.5 to pI 8.5
  • Mini
150 min 2 months

Zymogram

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
Zymogram
  • Separation of proteins based on size and activity
10% (with gelatin) 10 to 220 kDa
  • Mini
90 min 2 months

E-PAGE

Precast gels When to use Gel % available Separation range Formats available Average run time Shelf life
E-PAGE
  • High throughput for recombination product analysis and protein profiling
E-PAGE 96:6%
E-PAGE 48:8%
10 to 220 kDa
  • 48-well
  • 96-well
14–25 min 6 months
  NativePAGE Bis-Tris Gels
NativePAGE Bis-Tris Gels
View all
Isoelectric Focusing Gels
Isoelectric Focusing Gels
View all
Novex Zymogram Gels
Novex Zymogram Gels
View all
E-PAGE Precast Gels
E-PAGE Precast
Gels

View all
Gel chemistry Bis-Tris pH 3–7, pH 3–10 Tris-glycine with gelatin 8% or 6%
Protein sample type 15 to 10,000 kDa pI 3.5 to pI 8.5 10 to 220 kDa 10-200 kDa
Product benefits Separation of native proteins, neutral pH for high sensitivity applications Separation of proteins based on isoelectric point Separation of proteins based on size and activity High-throughput gel system, that eliminates the need for buffers
  SureCast Handcast System
SureCast Handcast System
Explore
Empty Gel Cassettes
Empty Gel Cassettes
Explore
Bolt Empty Cassettes
Bolt Empty Cassettes
Explore
Cassette type Resuable, glass Single use, pre-sealed Single use, pre-sealed
Product benefits
  • Leak-free design—minimize the number of failed gels, waste less time
  • Superior glass plate durability
  • Unique tilt feature—minimize spillage and confidently load acrylamide solutions
  • Simple component assembly—using a single-motion load-and-lock mechanism
  • Ready-to-pour cassettes, casting stand not required, cassettes sealed on three sides (no gel leakage)
  • Special coating provides uniform polymerization rate for a more consistent handcast gel
  • High capacity sample wells- load up to 60 µL per well
  • Ready-to-pour cassettes, casting stand not required, cassettes sealed on three sides (no gel leakage)
  • Special coating provides uniform polymerization rate for a more consistent handcast gel
Gel dimensions Mini: 8x8 cm (1 mm thick)

Mini: 8x8 cm (1 or 1.5 mm thick)

Midi: 8X13 cm (1 mm thick)

Mini: 8x8 cm (1 mm thick)
Gel tank Mini Gel Tank, Xcell SureLock Mini-Cell

Mini Gels: Mini Gel Tank or XCell SureLock Mini-Cell

Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only)

Mini Gel Tank, Xcell SureLock Mini-Cell
Reagents SureCast reagents, other popular polyacrylamide reagents SureCast reagents, other popular polyacrylamide reagents SureCast reagents, other popular polyacrylamide reagents
Combos available 1, 2, 10, 12, 15-wells 1, 2, 5, 10, 12, 15 and 2D-wells 10 or 12-wells (high capacity wells)

Polyacrylamide gels

All Invitrogen precast protein gels are high quality polyacrylamide gels designed to deliver superior performance, reliability, reproducibility, and consistency of quality. Our extensive portfolio of precast protein gels provides a wide variety of gel options available for your research. Whether your protein is 2.5 kDa or as large as 500 kDa, or you’re looking for separation under native conditions or by isoelectric point—we have the right gel for your application.

Our precast protein polyacrylamide gels are offered in four different chemistries. The choice of whether to use one chemistry or another depends on the abundance of the protein you’re separating, the size of the protein, and your downstream application.

SDS PAGE protein separation

For denaturing gel electrophoresis or SDS PAGE there are many options available based on the application, and size of the protein of interest. For separation of a broad range of proteins, two chemistries, Bis-Tris and Tris-glycine, have been optimized for performance and long shelf life. Choose Bis-Tris gel chemistry when you have a low abundance of protein or when your downstream applications require high protein integrity, such as posttranslational modification analysis, mass spectrometry, or sequencing. Bis-Tris chemistry provides a neutral (pH 7.0) environment during electrophoresis, which may result in better sample integrity and stability of the gel. This helps reduce protein modifications and helps produce sharp band resolution. Bis-Tris gel chemistry is offered in our Bolt Bis-Tris Plus and NuPAGE Bis-Tris gels. Bolt Bis-Tris Plus mini gels build on the legacy of the highly published NuPAGE Bis-Tris gels and feature larger capacity wells- for loading up to 60 µl per well.

To separate high-abundance proteins, select our robust Novex Tris-Glycine gel chemistry, which offers maximum protein separation performance and crisp, straight bands.

For separation of high molecular weight proteins, Tris-acetate gel chemistry is recommended, offered in NuPAGE Tris-Acetate gels.

For separation of low molecular weight proteins, use Novex Tricine gels which provide increased resolution of proteins with molecular weights as low as 2.5 kDa.

Native PAGE protein separation

For separation of proteins under nondenaturing conditions or native page, three gel systems are recommended- NativePAGE Bis-Tris, Novex Tris-Glycine and NuPAGE Tris-Acetate. NativePAGE Bis-Tris gels, are based on the blue native polyacrylamide gel electrophoresis (BN-PAGE) which helps overcome the limitations of traditional native electrophoresis by providing a near-neutral operating pH and detergent compatibility. Both Novex Tris-Glycine and NuPAGE Tris-Acetate gels can be run under native and non-reducing conditions for separation of proteins by net charge, size and shape.

For separation of proteins by isoelectroctric point (pI), use Novex IEF gels. Novex IEF gels effectively create a pH gradient to separate proteins according to their unique pI.

Need help determining the best gel to use?
Protein Gel Selection Guide

Protein Gel Selection Guide

Find the exact gel and buffers needed for your electrophoresis experiments.

Gel Selection Chart

Gel Selection Chart

Find the right gel for your research needs based on molecular weight, downstream applications, and throughput requirements.

Protein Gel Options Brochure

Protein Gel Options Brochure

Learn how the right protein gel chemistry can help you achieve better protein separation and western blots.

Protein Gels Performance Guarantee

Protein Gels Performance Guarantee

We stand behind the performance of our protein gels.

Learn more

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