Protein Gels

Explore our protein gel options. Choose from precast polyacrylamide gel electrophoresis (PAGE) chemistries designed for specific applications, each available in a variety of well and cassette formats, or select a system for pouring and casting your own gels.

Find the right gel for your protein
Convert from other suppliers' gels

 Bis-Tris Gels
Bis-Tris Gels
Tris-Glycine Gels
Tris-Glycine Gels
Tris-Acetate Gels
Tris-Acetate Gels
Tricine Gels
Tricine Gels
Available precast gelsNuPAGE Bis-Tris Gels, Bolt Bis-Tris Plus GelsNovex Tris-GlycineNuPAGE Tris-AcetateNovex Tricine
Protein sample typeBroad range MW (6-400 kDa)Broad range MW (6-400 kDa)High range MW (40-500 kDa)Low range MW (2.5-40 kDa)
Product benefitsNeutral pH for high sensitivity applications such as western blotting and mass spectrometry. Load up to 60 µL per well with Bolt gels. Run times as fast as 20–35 minutes.Traditional Laemmli-style gels with larger loading capacity-load up to 60 µL per well.Neutral pH for better separation, transfer, and analysis of high molecular weight proteins.Better separation, transfer, and analysis of low molecular weight proteins.
Recommended forWestern blotting, mass spectrometry, post translationally modified proteins, dilute samples, and denaturing applications.Western blotting, in-gel staining, samples containing detergent and high salt, and denaturing or native PAGE applications.High molecular weight proteins, Western blotting, mass spectrometry, post translationally modified proteins, and denaturing or native PAGE applications.Low molecular weight proteins, western blotting, in-gel staining, and denaturing applications.

Bis-Tris

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage run timeShelf life
Bolt Bis-Tris Plus
  • Dilute or larger (up to 60 µL) samples 
  • Separation of small- to medium-sized proteins
  • Western blot transfer and analysis, and all techniques in which protein integrity is crucial
8%, 10%, 12%, 4–12% 6 to 400 kDa
  • Mini with larger well capacity (WedgeWell, up to 60 µL)
22–45 minup to 16 months
NuPAGE Bis-Tris
  • Separation of small- to medium-sized proteins
  • Western blot transfer and analysis, and all techniques in which protein integrity is crucial
8%, 10%, 12%, 4–12% 1.5 to 300 kDa
  • Mini
  • Midi
35–50 min12 months

Tris-Glycine

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage run timeShelf life

Novex Tris-Glycine

 

  • Separation of small- to medium-sized proteins using traditional Laemmli-style gels

6%, 8%, 10%, 12%,

4–20%, 4–12%, 14%,

16%, 8–16% , 10–20%

8 to 250 kDa

  • Mini with larger well capacity (WedgeWell, up to 60 µL)
  • Midi

90 min

up to 12 months

Tris-Acetate

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage run timeShelf life
NuPAGE Tris-Acetate
  • Separation of larger proteins
7%, 3–8%36 to 500 kDa
  • Mini
  • Midi
60 min6 months

Tricine

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage run timeShelf life
Novex Tricine
  • Separation of small proteins and peptides
10%, 16%2 to 200 kDa
  • Mini
90 min1–2 months

Native Bis-Tris

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage run timeShelf life

NativePAGE Bis-Tris

  • Separation of native proteins
3–12%, 4–16%15 to 10,000 kDa
  • Mini
90–120 min12 months

IEF

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage run timeShelf life
IEF
  • Separation of proteins based on isoelectric point
pH 3–7, pH 3–10pI 3.5 to pI 8.5
  • Mini
150 min2 months

Zymogram

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage run timeShelf life
Zymogram
  • Separation of proteins based on size and activity
10% (with gelatin)10 to 220 kDa
  • Mini
90 min2 months

E-PAGE

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage run timeShelf life
E-PAGE
  • High throughput for recombination product analysis and protein profiling
E-PAGE 96:6%
E-PAGE 48:8%
10 to 220 kDa
  • 48-well
  • 96-well
14–25 min6 months
 NativePAGE Bis-Tris Gels
NativePAGE Bis-Tris Gels
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Isoelectric Focusing Gels
Isoelectric Focusing Gels
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Novex Zymogram Gels
Novex Zymogram Gels
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E-PAGE Precast Gels
E-PAGE Precast
Gels

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Gel chemistryBis-TrispH 3–7, pH 3–10Tris-glycine with gelatin8% or 6%
Protein sample type15 to 10,000 kDapI 3.5 to pI 8.510 to 220 kDa10-200 kDa
Product benefitsSeparation of native proteins, neutral pH for high sensitivity applicationsSeparation of proteins based on isoelectric pointSeparation of proteins based on size and activityHigh-throughput gel system, that eliminates the need for buffers
 SureCast Handcast System
SureCast Handcast System
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Empty Gel Cassettes
Empty Gel Cassettes
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Bolt Empty Cassettes
Bolt Empty Cassettes
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Cassette typeResuable, glassSingle use, pre-sealedSingle use, pre-sealed
Product benefits
  • Leak-free design—minimize the number of failed gels, waste less time
  • Superior glass plate durability
  • Unique tilt feature—minimize spillage and confidently load acrylamide solutions
  • Simple component assembly—using a single-motion load-and-lock mechanism
  • Ready-to-pour cassettes, casting stand not required, cassettes sealed on three sides (no gel leakage)
  • Special coating provides uniform polymerization rate for a more consistent handcast gel
  • High capacity sample wells- load up to 60 µL per well
  • Ready-to-pour cassettes, casting stand not required, cassettes sealed on three sides (no gel leakage)
  • Special coating provides uniform polymerization rate for a more consistent handcast gel
Gel dimensionsMini: 8x8 cm (1 mm thick)

Mini: 8x8 cm (1 or 1.5 mm thick)

Midi: 8X13 cm (1 mm thick)

Mini: 8x8 cm (1 mm thick)
Gel tankMini Gel Tank, Xcell SureLock Mini-Cell

Mini Gels: Mini Gel Tank or XCell SureLock Mini-Cell

Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only)

Mini Gel Tank, Xcell SureLock Mini-Cell
ReagentsSureCast reagents, other popular polyacrylamide reagentsSureCast reagents, other popular polyacrylamide reagentsSureCast reagents, other popular polyacrylamide reagents
Combos available1, 2, 10, 12, 15-wells1, 2, 5, 10, 12, 15 and 2D-wells10 or 12-wells (high capacity wells)
 Bis-Tris Gels
Bis-Tris Gels
Tris-Glycine Gels
Tris-Glycine Gels
Tris-Acetate Gels
Tris-Acetate Gels
Tricine Gels
Tricine Gels
Available precast gelsNuPAGE Bis-Tris Gels, Bolt Bis-Tris Plus GelsNovex Tris-GlycineNuPAGE Tris-AcetateNovex Tricine
Protein sample typeBroad range MW (6-400 kDa)Broad range MW (6-400 kDa)High range MW (40-500 kDa)Low range MW (2.5-40 kDa)
Product benefitsNeutral pH for high sensitivity applications such as western blotting and mass spectrometry. Load up to 60 µL per well with Bolt gels. Run times as fast as 20–35 minutes.Traditional Laemmli-style gels with larger loading capacity-load up to 60 µL per well.Neutral pH for better separation, transfer, and analysis of high molecular weight proteins.Better separation, transfer, and analysis of low molecular weight proteins.
Recommended forWestern blotting, mass spectrometry, post translationally modified proteins, dilute samples, and denaturing applications.Western blotting, in-gel staining, samples containing detergent and high salt, and denaturing or native PAGE applications.High molecular weight proteins, Western blotting, mass spectrometry, post translationally modified proteins, and denaturing or native PAGE applications.Low molecular weight proteins, western blotting, in-gel staining, and denaturing applications.

Bis-Tris

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage run timeShelf life
Bolt Bis-Tris Plus
  • Dilute or larger (up to 60 µL) samples 
  • Separation of small- to medium-sized proteins
  • Western blot transfer and analysis, and all techniques in which protein integrity is crucial
8%, 10%, 12%, 4–12% 6 to 400 kDa
  • Mini with larger well capacity (WedgeWell, up to 60 µL)
22–45 minup to 16 months
NuPAGE Bis-Tris
  • Separation of small- to medium-sized proteins
  • Western blot transfer and analysis, and all techniques in which protein integrity is crucial
8%, 10%, 12%, 4–12% 1.5 to 300 kDa
  • Mini
  • Midi
35–50 min12 months

Tris-Glycine

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage run timeShelf life

Novex Tris-Glycine

 

  • Separation of small- to medium-sized proteins using traditional Laemmli-style gels

6%, 8%, 10%, 12%,

4–20%, 4–12%, 14%,

16%, 8–16% , 10–20%

8 to 250 kDa

  • Mini with larger well capacity (WedgeWell, up to 60 µL)
  • Midi

90 min

up to 12 months

Tris-Acetate

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage run timeShelf life
NuPAGE Tris-Acetate
  • Separation of larger proteins
7%, 3–8%36 to 500 kDa
  • Mini
  • Midi
60 min6 months

Tricine

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage run timeShelf life
Novex Tricine
  • Separation of small proteins and peptides
10%, 16%2 to 200 kDa
  • Mini
90 min1–2 months

Native Bis-Tris

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage run timeShelf life

NativePAGE Bis-Tris

  • Separation of native proteins
3–12%, 4–16%15 to 10,000 kDa
  • Mini
90–120 min12 months

IEF

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage run timeShelf life
IEF
  • Separation of proteins based on isoelectric point
pH 3–7, pH 3–10pI 3.5 to pI 8.5
  • Mini
150 min2 months

Zymogram

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage run timeShelf life
Zymogram
  • Separation of proteins based on size and activity
10% (with gelatin)10 to 220 kDa
  • Mini
90 min2 months

E-PAGE

Precast gelsWhen to useGel % availableSeparation rangeFormats availableAverage run timeShelf life
E-PAGE
  • High throughput for recombination product analysis and protein profiling
E-PAGE 96:6%
E-PAGE 48:8%
10 to 220 kDa
  • 48-well
  • 96-well
14–25 min6 months
 NativePAGE Bis-Tris Gels
NativePAGE Bis-Tris Gels
View all
Isoelectric Focusing Gels
Isoelectric Focusing Gels
View all
Novex Zymogram Gels
Novex Zymogram Gels
View all
E-PAGE Precast Gels
E-PAGE Precast
Gels

View all
Gel chemistryBis-TrispH 3–7, pH 3–10Tris-glycine with gelatin8% or 6%
Protein sample type15 to 10,000 kDapI 3.5 to pI 8.510 to 220 kDa10-200 kDa
Product benefitsSeparation of native proteins, neutral pH for high sensitivity applicationsSeparation of proteins based on isoelectric pointSeparation of proteins based on size and activityHigh-throughput gel system, that eliminates the need for buffers
 SureCast Handcast System
SureCast Handcast System
Explore
Empty Gel Cassettes
Empty Gel Cassettes
Explore
Bolt Empty Cassettes
Bolt Empty Cassettes
Explore
Cassette typeResuable, glassSingle use, pre-sealedSingle use, pre-sealed
Product benefits
  • Leak-free design—minimize the number of failed gels, waste less time
  • Superior glass plate durability
  • Unique tilt feature—minimize spillage and confidently load acrylamide solutions
  • Simple component assembly—using a single-motion load-and-lock mechanism
  • Ready-to-pour cassettes, casting stand not required, cassettes sealed on three sides (no gel leakage)
  • Special coating provides uniform polymerization rate for a more consistent handcast gel
  • High capacity sample wells- load up to 60 µL per well
  • Ready-to-pour cassettes, casting stand not required, cassettes sealed on three sides (no gel leakage)
  • Special coating provides uniform polymerization rate for a more consistent handcast gel
Gel dimensionsMini: 8x8 cm (1 mm thick)

Mini: 8x8 cm (1 or 1.5 mm thick)

Midi: 8X13 cm (1 mm thick)

Mini: 8x8 cm (1 mm thick)
Gel tankMini Gel Tank, Xcell SureLock Mini-Cell

Mini Gels: Mini Gel Tank or XCell SureLock Mini-Cell

Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only)

Mini Gel Tank, Xcell SureLock Mini-Cell
ReagentsSureCast reagents, other popular polyacrylamide reagentsSureCast reagents, other popular polyacrylamide reagentsSureCast reagents, other popular polyacrylamide reagents
Combos available1, 2, 10, 12, 15-wells1, 2, 5, 10, 12, 15 and 2D-wells10 or 12-wells (high capacity wells)

Polyacrylamide gels

All Invitrogen precast protein gels are high quality polyacrylamide gels designed to deliver superior performance, reliability, reproducibility, and consistency of quality. Our extensive portfolio of precast protein gels provides a wide variety of gel options available for your research. Whether your protein is 2.5 kDa or as large as 500 kDa, or you’re looking for separation under native conditions or by isoelectric point—we have the right gel for your application.

Our precast protein polyacrylamide gels are offered in four different chemistries. The choice of whether to use one chemistry or another depends on the abundance of the protein you’re separating, the size of the protein, and your downstream application.

SDS PAGE protein separation

For denaturing gel electrophoresis or SDS PAGE there are many options available based on the application, and size of the protein of interest. For separation of a broad range of proteins, two chemistries, Bis-Tris and Tris-glycine, have been optimized for performance and long shelf life. Choose Bis-Tris gel chemistry when you have a low abundance of protein or when your downstream applications require high protein integrity, such as posttranslational modification analysis, mass spectrometry, or sequencing. Bis-Tris chemistry provides a neutral (pH 7.0) environment during electrophoresis, which may result in better sample integrity and stability of the gel. This helps reduce protein modifications and helps produce sharp band resolution. Bis-Tris gel chemistry is offered in our Bolt Bis-Tris Plus and NuPAGE Bis-Tris gels. Bolt Bis-Tris Plus mini gels build on the legacy of the highly published NuPAGE Bis-Tris gels and feature larger capacity wells- for loading up to 60 µl per well.

To separate high-abundance proteins, select our robust Novex Tris-Glycine gel chemistry, which offers maximum protein separation performance and crisp, straight bands.

For separation of high molecular weight proteins, Tris-acetate gel chemistry is recommended, offered in NuPAGE Tris-Acetate gels.

For separation of low molecular weight proteins, use Novex Tricine gels which provide increased resolution of proteins with molecular weights as low as 2.5 kDa.

Native PAGE protein separation

For separation of proteins under nondenaturing conditions or native page, three gel systems are recommended- NativePAGE Bis-Tris, Novex Tris-Glycine and NuPAGE Tris-Acetate. NativePAGE Bis-Tris gels, are based on the blue native polyacrylamide gel electrophoresis (BN-PAGE) which helps overcome the limitations of traditional native electrophoresis by providing a near-neutral operating pH and detergent compatibility. Both Novex Tris-Glycine and NuPAGE Tris-Acetate gels can be run under native and non-reducing conditions for separation of proteins by net charge, size and shape.

For separation of proteins by isoelectroctric point (pI), use Novex IEF gels. Novex IEF gels effectively create a pH gradient to separate proteins according to their unique pI.

How-to videos

How to choose the right protein gel for your research application

This webinar will highlight the different protein modifications occurring during electrophoresis, the importance of pH in sample integrity, how to choose the right gel chemistry for specific research applications, and how to introduce the right new gel chemistry in your research workflow.

How to separate proteins using an Invitrogen precast SDS-PAGE gel

This video will show you how to separate proteins by using Invitrogen Mini Gel Tank and Invitrogen precast SDS-PAGE gels.

Need help determining the best gel to use?
Protein Gels Performance Guarantee

Protein Gels Performance Guarantee

We stand behind the performance of our protein gels.

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