Novex Tris-Glycine Gels

Invitrogen Novex Tris-Glycine Gels are robust gels for a wide range of sample types and molecular weights. They offer sharp straight bands and easier-to-load, larger capacity wells, and can be used for denaturing or native PAGE applications.

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Transition from old to new Novex Tris-Glycine Gel format

Novex Tris-Glycine Gel specifications

Available gel sizes Mini: 8 cm x 8 cm (1.0 mm thick)
Midi: 8 cm x 13 cm (1.0 mm thick)
Storage conditions 2–8°C
Shelf life Up to 12 months
Recommended sample buffer SDS-PAGE: Novex Tris-Glycine SDS Sample Buffer
Native-PAGE: Novex Tris-Glycine Native Sample Buffer
Recommended running buffers SDS-PAGE: Novex Tris-Glycine SDS Running Buffer
Native-PAGE: Novex Tris-Glycine Native Running Buffer
Recommended transfer buffers Novex Tris-Glycine Transfer Buffer
Gel chemistry Tris-glycine
Available polyacrylamide concentrations 6%, 8%, 10%, 12%, 14%, 16%, 4–12%, 4–20%, 8–16%, 10–20%
Separation range (denaturing) 8–250 kDa
For use with (equipment) mini gels Mini Gel Tank or XCell SureLock Mini-Cell
For use with (equipment) midi gels Invitrogen XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only)
Mode of separation SDS-PAGE: Molecular weight
Native-PAGE: Intrinsic charge, molecular size
Applications SDS-PAGE, Native-PAGE
Well type* Mini: 1D, WedgeWell format (load up to 60 µL per well)
Midi: 1D

*Not all percentages are available in every well type

High capacity wells

The unique wedge-shaped well in every Invitrogen Novex Tris-Glycine Mini Gel provides higher loading capacity, so you can load up to twice as much protein sample in every well.

Well size Recommended loading volume Maximum loading volume Maximum protein load
10-well 40 µL 60 µL 0.5 µg/band
12-well 30 µL 45 µL 0.4 µg/band
15-well 20 µL 35 µL 0.25 µg/band
17-well 15 µL 30 µL 0.2 µg/band

Detect proteins even in dilute samples or measure expression of low-abundance proteins. With more room to load your sample, you no longer have to worry about sample spillover and cross-well contamination on your western blots. Gel loading is also easier with no need to use special gel-loading pipette tips.

Increased sample volume capacity of Novex Tris-Glycine Gels with high capacity WedgeWells

Increased sample volume capacity of Novex Tris-Glycine Gels, WedgeWell format. (A) Increasing volumes (20 μL–60 μL) of a fluorescent protein ladder were loaded in every other lane of an Invitrogen Novex Tris-Glycine 10-well Gel. (B) Increasing volumes (20 μL–60 μL) of the same fluorescent protein ladder were loaded in every other lane of supplier B gel. In gel B, sample spillover is observed as signal in lanes adjacent to the 50-μL and 60-μL load lanes.

Invitrogen Novex Tris-Glycine Mini Gels and Novex Tris-Glycine Plus Midi Gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, which allows the use of Laemmli sample and running buffers. Novex Tris-Glycine Gels offer reproducible separation of a wide range of proteins into well-resolved bands.

Features of the Novex Tris-Glycine Gels include:

  • Diversity—use for native and denaturing protein assays
  • Wedge-shaped wells—easily load up to two times more sample volume (Mini only)
  • Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes
  • Improved shelf life—store gels for up to 12 months at 4°C

Novex Tris-Glycine Gels do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using tris-glycine SDS sample buffer and a tris-glycine SDS running buffer. For native proteins, we recommend using a tris-glycine native sample buffer and a tris-glycine native running buffer.

Novex Tris-Glycine Gels use a tris-glycine discontinuous buffer system with three ions primarily involved:

  • Chloride (–), supplied by the gel buffer, serves as the leading ion because it has the highest attraction to the anode relative to other anions in the system.
  • Glycine (–), the primary anion provided by the running buffer, serves as the trailing ion, because it is only partially negatively charged and remains behind the more highly charged chloride ions in a charged environment.
  • Tris base (+) is a common ion present in both the gel and the running buffers. During electrophoresis, the gel and buffer ions in the tris-glycine system form an operating pH of 9.5 in the separating region of the gel.

Novex Tris-Glycine Gel band quality

Novex Tris-Glycine Gels are designed to deliver well-resolved, straight bands with optimal band quality as compared to other commercially available precast gels.

High band quality is observed with Novex Tris-glycine gel compared to other commercial suppliers

Band quality with Novex Tris-Glycine gels. Protein ladders, purified proteins, and E. coli lysate were loaded on an Invitrogen Novex Tris-Glycine Gel, 4–20% gradient (A) and a supplier 4–20% gradient gel (B). Straighter lanes with better lysate protein band sharpness and resolution are observed on gel (A). Lanes 1, 5, 10: 5 µL Thermo Scientific PageRuler Unstained Protein Ladder; lanes 2, 6, 9: 5 µL Invitrogen Mark12 Unstained Standard; lane 3: 10 µg E. coli lysate; lane 4: 6 µg BSA; lane 7: 6 µg hIgG; lane 8: 20 µg E. coli lysate.

Novex Tris-glycine gels deliver sharp straight bands

Novex Tris-Glycine Gels deliver sharp straight bands. Protein ladders and A431 cell lysate were loaded on a Novex Tris-Glycine Gel, 4–20% gradient and transferred to nitrocellulose using the Invitrogen iBlot 2 Gel Transfer Device. Lane 1: Invitrogen iBright Prestained Protein Ladder; Lane 2: Invitrogen MagicMark XP Western Protein Standard; Lanes 3–7: A431 cell lysate, 15 µg, 5 µg, 1.67 µg, 0.55 µg, 0.19 µg.

Protein integrity

With Novex Tris-Glycine Gels, you can achieve greater protein integrity as compared to other commercially available precast gels.

Novex Tris-Glycine gels offer increased protein integrity over other commercial gel suppliers

Novex Tris-Glycine Gels offer increased protein integrity. Protein ladder, purified proteins, and E. coli lysate were loaded on a 4–20% gradient Novex Tris-Glycine Gel (A) and a supplier 4–20% gradient gel (B). Gel (B) displays numerous low molecular weight protein degradation products below major bands in lanes 3, 4, 7, 8. These are not seen in gel (A). Gel (A) also displays better lysate protein band sharpness and resolution than gel (B). Lanes 1, 10: 5 µL Mark12 Unstained Standard; lane 2: 10 µg E. coli lysate; lane 3: 6 µg catalase; lane 4: 6 µg carbonic anhydrase; lane 5: 6 µg lysozyme; lane 6: 6 µg hIgM ; lane 7: 6 µg BSA; lane 8: 6 µg beta-galactosidase; lane 9: 20 µg E. coli lysate.

Migration patterns for Novex Tris-Glycine Gels

Mini Gels

Migration pattern for Novex Tris-Glycine Mini gels

Midi Gels

Migration pattern for Novex Tris-glycine Plus midi gels

Novex Tris-Glycine Gel specifications

Available gel sizes Mini: 8 cm x 8 cm (1.0 mm thick)
Midi: 8 cm x 13 cm (1.0 mm thick)
Storage conditions 2–8°C
Shelf life Up to 12 months
Recommended sample buffer SDS-PAGE: Novex Tris-Glycine SDS Sample Buffer
Native-PAGE: Novex Tris-Glycine Native Sample Buffer
Recommended running buffers SDS-PAGE: Novex Tris-Glycine SDS Running Buffer
Native-PAGE: Novex Tris-Glycine Native Running Buffer
Recommended transfer buffers Novex Tris-Glycine Transfer Buffer
Gel chemistry Tris-glycine
Available polyacrylamide concentrations 6%, 8%, 10%, 12%, 14%, 16%, 4–12%, 4–20%, 8–16%, 10–20%
Separation range (denaturing) 8–250 kDa
For use with (equipment) mini gels Mini Gel Tank or XCell SureLock Mini-Cell
For use with (equipment) midi gels Invitrogen XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only)
Mode of separation SDS-PAGE: Molecular weight
Native-PAGE: Intrinsic charge, molecular size
Applications SDS-PAGE, Native-PAGE
Well type* Mini: 1D, WedgeWell format (load up to 60 µL per well)
Midi: 1D

*Not all percentages are available in every well type

High capacity wells

The unique wedge-shaped well in every Invitrogen Novex Tris-Glycine Mini Gel provides higher loading capacity, so you can load up to twice as much protein sample in every well.

Well size Recommended loading volume Maximum loading volume Maximum protein load
10-well 40 µL 60 µL 0.5 µg/band
12-well 30 µL 45 µL 0.4 µg/band
15-well 20 µL 35 µL 0.25 µg/band
17-well 15 µL 30 µL 0.2 µg/band

Detect proteins even in dilute samples or measure expression of low-abundance proteins. With more room to load your sample, you no longer have to worry about sample spillover and cross-well contamination on your western blots. Gel loading is also easier with no need to use special gel-loading pipette tips.

Increased sample volume capacity of Novex Tris-Glycine Gels with high capacity WedgeWells

Increased sample volume capacity of Novex Tris-Glycine Gels, WedgeWell format. (A) Increasing volumes (20 μL–60 μL) of a fluorescent protein ladder were loaded in every other lane of an Invitrogen Novex Tris-Glycine 10-well Gel. (B) Increasing volumes (20 μL–60 μL) of the same fluorescent protein ladder were loaded in every other lane of supplier B gel. In gel B, sample spillover is observed as signal in lanes adjacent to the 50-μL and 60-μL load lanes.

Invitrogen Novex Tris-Glycine Mini Gels and Novex Tris-Glycine Plus Midi Gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, which allows the use of Laemmli sample and running buffers. Novex Tris-Glycine Gels offer reproducible separation of a wide range of proteins into well-resolved bands.

Features of the Novex Tris-Glycine Gels include:

  • Diversity—use for native and denaturing protein assays
  • Wedge-shaped wells—easily load up to two times more sample volume (Mini only)
  • Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes
  • Improved shelf life—store gels for up to 12 months at 4°C

Novex Tris-Glycine Gels do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using tris-glycine SDS sample buffer and a tris-glycine SDS running buffer. For native proteins, we recommend using a tris-glycine native sample buffer and a tris-glycine native running buffer.

Novex Tris-Glycine Gels use a tris-glycine discontinuous buffer system with three ions primarily involved:

  • Chloride (–), supplied by the gel buffer, serves as the leading ion because it has the highest attraction to the anode relative to other anions in the system.
  • Glycine (–), the primary anion provided by the running buffer, serves as the trailing ion, because it is only partially negatively charged and remains behind the more highly charged chloride ions in a charged environment.
  • Tris base (+) is a common ion present in both the gel and the running buffers. During electrophoresis, the gel and buffer ions in the tris-glycine system form an operating pH of 9.5 in the separating region of the gel.

Novex Tris-Glycine Gel band quality

Novex Tris-Glycine Gels are designed to deliver well-resolved, straight bands with optimal band quality as compared to other commercially available precast gels.

High band quality is observed with Novex Tris-glycine gel compared to other commercial suppliers

Band quality with Novex Tris-Glycine gels. Protein ladders, purified proteins, and E. coli lysate were loaded on an Invitrogen Novex Tris-Glycine Gel, 4–20% gradient (A) and a supplier 4–20% gradient gel (B). Straighter lanes with better lysate protein band sharpness and resolution are observed on gel (A). Lanes 1, 5, 10: 5 µL Thermo Scientific PageRuler Unstained Protein Ladder; lanes 2, 6, 9: 5 µL Invitrogen Mark12 Unstained Standard; lane 3: 10 µg E. coli lysate; lane 4: 6 µg BSA; lane 7: 6 µg hIgG; lane 8: 20 µg E. coli lysate.

Novex Tris-glycine gels deliver sharp straight bands

Novex Tris-Glycine Gels deliver sharp straight bands. Protein ladders and A431 cell lysate were loaded on a Novex Tris-Glycine Gel, 4–20% gradient and transferred to nitrocellulose using the Invitrogen iBlot 2 Gel Transfer Device. Lane 1: Invitrogen iBright Prestained Protein Ladder; Lane 2: Invitrogen MagicMark XP Western Protein Standard; Lanes 3–7: A431 cell lysate, 15 µg, 5 µg, 1.67 µg, 0.55 µg, 0.19 µg.

Protein integrity

With Novex Tris-Glycine Gels, you can achieve greater protein integrity as compared to other commercially available precast gels.

Novex Tris-Glycine gels offer increased protein integrity over other commercial gel suppliers

Novex Tris-Glycine Gels offer increased protein integrity. Protein ladder, purified proteins, and E. coli lysate were loaded on a 4–20% gradient Novex Tris-Glycine Gel (A) and a supplier 4–20% gradient gel (B). Gel (B) displays numerous low molecular weight protein degradation products below major bands in lanes 3, 4, 7, 8. These are not seen in gel (A). Gel (A) also displays better lysate protein band sharpness and resolution than gel (B). Lanes 1, 10: 5 µL Mark12 Unstained Standard; lane 2: 10 µg E. coli lysate; lane 3: 6 µg catalase; lane 4: 6 µg carbonic anhydrase; lane 5: 6 µg lysozyme; lane 6: 6 µg hIgM ; lane 7: 6 µg BSA; lane 8: 6 µg beta-galactosidase; lane 9: 20 µg E. coli lysate.

Migration patterns for Novex Tris-Glycine Gels

Mini Gels

Migration pattern for Novex Tris-Glycine Mini gels

Midi Gels

Migration pattern for Novex Tris-glycine Plus midi gels
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