Discover a whole new depth to Tris-Glycine gels; Dive deeper with 60 µL load volume

Invitrogen™ Novex™ WedgeWell™ Tris-Glycine gels are precast mini gels for separation of proteins by polyacrylamide gel electrophoresis (PAGE) allowing the use of Laemmli sample and running buffers. These gels enable high quality performance and separation of a wide range of proteins into well-resolved bands. Novex WedgeWell Tris-Glycine gels are designed for use in our Invitrogen™ Mini Gel Tank.

Features of Novex WedgeWell Tris-Glycine gels:

  • Wedge-shaped wells—easily load up to 60 µL of sample per well
  • High performance—excellent protein band resolution and sharpness
  • Improved shelf life—store gels for up to 12 months at 4°C
  • Fast run conditions—quickly separate proteins using constant voltage in less than 60 minutes
  • Compatible with native and denatured protein samples

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Number of wells Gel percentage

Novex WedgeWell Tris-Glycine gels migration chart

For optimal and total separation ranges for each specific gel percentage, view the gel migration chart.

View the gel migration chart

Welcome pack: Novex WedgeWell Tris-Glycine Gel

Savings of up to 52%

Buy now, 10 well

10% gel pack  4-12% gel pack

Buy now, 15 well

10% gel pack  4-12% gel pack

  • Novex WedgeWell Tris-Glycine Gels, 2 boxes
  • Novex Tris-Glycine SDS Running Buffer (10X), 500 mL
  • Novex Tris-Glycine SDS Sample Buffer (2X), 20 mL
  • NuPAGE Sample Reducing Agent (10X), 250 uL
  • PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa 2*250uL
  • Mini Gel Tank

Product highlights

Increased sample volume capacity of WedgeWell Tris-Glycine gels. (Left) Increasing volumes (20 μL–60 μL) of a fluorescent protein ladder were loaded in every other lane of an Invitrogen™ Novex™ WedgeWell Tris-Glycine 10-well gel. (Right) Increasing volumes (20 μL–60 μL) of the same fluorescent protein ladder were loaded in every other lane of supplier gel (B). In gel (B), sample spillover is observed as signal in lanes adjacent to the 50 μL and 60 μL load lanes.

Band quality with Novex WedgeWell Tris-Glycine gels. Protein ladders, purified proteins and E. coli lysate were loaded on a 4–20% gradient WedgeWell Tris-Glycine gel (A) and a supplier 4–20% gradient gel (B). Straighter lanes with better lysate protein band sharpness and resolution are observed on gel (A). Lanes 1, 5, 10: 5 µL Thermo Scientific™ PageRuler™ Unstained Protein Ladder; lanes 2, 6, 9: 5 µL Mark12 Unstained Standard; lane 3: 10 µg E. coli lysate (10 µL sample volume); lane 4: 6 µg BSA (10 µL sample volume); lane 7: 6 µg hIgG (10 µL sample volume); lane 8: 20 µg E. coli lysate (20 µL sample volume

Greater protein integrity with Novex WedgeWell Tris-Glycine gels. Protein ladder, purified proteins, and E. coli lysate were loaded on a 4–20% gradient WedgeWell Tris-Glycine gel (A) and a supplier 4–20% gradient gel (B). Gel (B) displays numerous low molecular weight protein degradation products below major bands in lanes 3, 4, 7, 8. These are not seen in gel (A). Gel (A) also displays better lysate protein band sharpness and resolution than gel (B).  Lanes 1, 10: 5 µL Mark12 Unstained Standard; lane 2: 10 µg E. coli lysate (10 µL sample volume); lane 3: 6 µg catalase (10 µL sample volume); lane 4: 6 µg carbonic anhydrase (10 µL sample volume); lane 5: 6 µg lysozyme (10 µL sample volume); lane 6: 6 µg hIgM (10 µL sample volume); lane 7: 6 µg BSA (10 µL sample volume); lane 8: 6 µg beta-galactosidase (10 µL sample volume); lane 9: 20 µg E. coli lysate (20 µL sample volume).

Recommended loading volumes

Well type Recommended loading volume Maximum loading volume Maximum protein load
10 well 40 µL 60 µL 0.5 µg/band
12 well 30 µL 45 µL 0.4 µg/band
15 well 20 µL 35 µL 0.25 µg/band

Gel sizes

Novex™ WedgeWell Tris-Glycine gels are available in mini gels (8 cm x 8 cm). Mini gels are available in a 1.0 mm gel and feature the Invitrogen-exclusive WedgeWell® feature, giving them the largest sample volume capacity of any mini-sized 1.0mm trick Tris-Glycine gel. Novex™ WedgeWell Tris-Glycine gels come in 10-, 12- and 15-well formats. For loading volumes, see Recommended well loading volumes & sample loads.

Polyacrylamide concentrations

Novex WedgeWell Tris-Glycine gels come in a variety of fixed concentrations from 6% to 16%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. Each box contains 10 gels. To determine what type of gel should be used to separate your proteins, please refer to the protein migration patterns.

Gel storage and shelf-life

Novex WedgeWell Tris-Glycine gels should be stored at 4°C and have a shelf life of up to one year.

Run native or denatured proteins

Novex WedgeWell Tris-Glycine gels do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using Invitrogen™ Novex™ Tris-Glycine SDS Sample Buffer and Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Invitrogen™ Novex™ Tris-Glycine Native Sample Buffer and Invitrogen™ Novex™ Tris-Glycine Native Running Buffer.

Gel transfer

For transferring your proteins to membrane, we recommend using Invitrogen™ Novex™ Tris-Glycine Transfer Buffer. Transfers can be done using the Invitrogen™ Mini Blot Module or using the Invitrogen™ iBlot™ 2 Dry Blotting System.