Invitrogen precast midi gels are designed for higher-throughput electrophoresis with unparalleled quality

The Invitrogen line of precast midi gels are made with high-purity, strictly quality-controlled reagents to provide top-quality, consistent protein separation. Midi gels are a wider format gel (8 cm x 13 cm), designed for your higher-throughput electrophoresis needs.

Our midi gels run on the XCell4 SureLock Midi-Cell Chamber system or the Bio-Rad Criterion™ with adapters.

Precast midi gel options

  1. The NuPAGE SDS-PAGE Midi Gel System—a revolutionary neutral-pH, discontinuous SDS-PAGE system. The neutral (pH 7.0) environment during electrophoresis results in maximum stability of both proteins and gel matrix, providing better band resolution than other gel systems. This system has two gel formulations: NuPAGE Bis-Tris midi gels and NuPAGE Tris-Acetate midi gels.
  2. The NEW Novex Tris-Glycine Plus Midi Gel System—based on the Laemmli system with minor modifications for maximum performance in the precast format with extended shelf life and fast run times.

Both midi gel systems are available in different acrylamide concentrations and well formats. All gels are available in 1.0 mm thickness only. For loading volumes, see Recommended Well Loading Volumes & Sample Loads.

The new Novex Tris-Glycine Plus Midi gels provides high quality protein band resolution. Mark12 Unstained Standard, PageRuler Unstained Protein Ladder, E.coli lysate, purified catalase, HeLa lysate, purified IgG separated on a Novex 10% Tris-Glycine Plus Midi Gels, 20-well stained with commassie.

Gel chemistry Denaturing conditions Non-denaturing conditions (native) Features
NuPage Bis-Tris High performance gel, ideal for a broad range of molecular weight proteins and optimized for applications where protein integrity is crucial
NuPage Tris-acetate High performance gel, optimized for high molecular weight proteins
Novex Tris-Glycine Plus Broad-range molecular weight separation

Choose the right gel

Click the image above to enlarge the midi gel migration charts, or download here.


Available NuPAGE Bis-Tris midi gels

Number of wells Gel percentage Gel sizes

Available NuPAGE Tris-Acetate midi gels

Number of wells Gel percentage Gel sizes

Available Novex Tris-Glycine Plus midi gels

Number of wells Gel percentage Gel sizes

Current users of Novex Tris-Glycine midi gels: You can expect the same high quality results with the new Novex Tris-Glycine midi gels. In addition, you can now enjoy the added benefit of longer shelf life.

Click the image above to enlarge the Novex Tris-Glycine Plus midi gel migration chart, or download here.
See conversion chart here.