Protein Gel Casting Cassettes

Pour your own polyacrylamide (SDS-PAGE) mini or midi gels with our sealed empty gel cassettes. These cassettes give you the option of hand casting your own gels while enjoying the benefits of the Mini Gel Tank and SureLock systems.

 

Bolt Mini Gel Cassettes

Novex Empty Cassettes

Size availableMini Gel: 8x8cm (1.0 mm thick)Mini gel: 8x8 cm (1.5 or 1.0 mm thick)
Midi gel: 8x13 cm (1.0 mm thick)
Equipment requiredMini Gel Tank or XCell SureLock Mini-CellMini Gels: Mini Gel Tank or XCell SureLock Mini-Cell
Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only)
Combs available10 and 12-well Wedgewell (high capacity sample well)1, 2, 2D, 5, 10, 12, 15-well
Product benefits
  • Load up to 60µL per well
  • Sealed on three sides for no gel leaking
  • No casting stand required
  • Special coating provides an uniform polymerization rate for a more consistent handcast gel
  • Sealed on three sides for no gel leaking
  • No casting stand required
  • Special coating provides an uniform polymerization rate for a more consistent handcast gel

Recommended maximum loading volumes

Well Type1.0 mm thickness1.5 mm thickness
10-well Wedgewell60 μL 
12-well Wedgewell45 μL 
1-well700 μL 
5-well60 μL 
10-well25 µL37 µL
12-well20 µL 
15-well15 µL25 µL
2D-well400 µL 
12+2* well45 µL + 15 µL 
20* well25 µL 
26* well15 µL 

* Midi Gel

Gel casting recipes

Choose the polyacrylamide percentage and type of well for your application based upon the expected molecular weight of your protein of interest and the volume of your sample.

Resolve large molecules with low percentage gels and small molecules with high percentage gels. If the molecular weight of the molecule is unknown, or the sample contains a wide range of molecules, use a mid-range percentage gel.

Recipes for SureCast reagents

Prepare resolving gel solution according to the volumes in the following table. The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast.

Note: Solutions do not require degassing.

 Polyacrylamide %
Solution4%6%8%10%12%14%16%18%20%
SureCast Acrylamide (40%)0.8 mL1.2 mL1.6 mL2.0 mL2.4 mL2.8 mL3.3 mL3.6 mL4.0 mL
SureCast Resolving Buffer2.0 mL2.0 mL2.0 mL2.0 mL2.0 mL2.0 mL2.0 mL2.0 mL2.0 mL
Distilled water5.1 mL4.7 mL4.3 mL3.9 mL3.5 mL3.1 mL2.7 mL2.3 mL1.9 mL
10% SureCast APS80 µL80 µL80 µL80 µL80 µL80 µL80 µL80 µL80 µL
SureCast TEMED**8 µL8 µL8 µL8 µL8 µL8 µL8 µL8 µL8 µL

** Add this last and mix well just before the gel is to be poured

Prepare stacking gel solution according to the following table. The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast. Note: Solutions do not require degassing.

Solution4%
SureCast Acrylamide (40%)0.30 mL
SureCast Stacking Buffer0.75 mL
Distilled water1.92 mL
10% SureCast APS30 µL
SureCast TEMED**3 µL
** Add this last and mix well just before the gel is to be poured

Recipes for standalone reagents

Prepare the following stock solutions: All solutions can be stored at room temperature.

50% Acrylamide/BIS (29:1)

  • 48.3 g Acrylamide
  • 1.7 g Bis-acrylamide
Bring to 100 mL with water
Store up to two months in a dark glass bottle

Separating Gel Buffer (1 M Tris-HCl, pH 8.8)

  • Add 30.3 g Tris to 150 mL water
  • Adjust pH 8.8 with HCL
Bring to 250 mL with water

Stacking Gel Buffer (0.375M Tris HCl, pH 6.8)

  • Add 11.4 g Tris to about 150 mL water
  • Adjust to pH 6.8 with HCl
Bring to 250 mL with water

Catalyst- Ammonium Persulfate (Make fresh the day of use)

  • 100 mg Ammonium Persulfate
  • Bring to 2 mL in water

10% SDS

  • 10.0g SDS
Bring to 100 mL with water

50% Sucrose

  • 50.0 g Sucrose
Bring to 100 mL with water
  

Separating gels

The following recipes are for approximately 25 mL of separating gels, enough for four 1.0-mm thick mini gels. Scale volumes proportionally based on the number of gels to be cast.

Solution 6% Gel 8% Gel10% Gel12% Gel14% Gel16% Gel18% Gel20% Gel
50% Acrylamide/BIS3.0 mL4.0 mL5.0 mL6.0 mL7.0 mL8.0 mL9.0 mL10.0 mL
Separating Gel Buffer9.4 mL9.4 mL9.4 mL9.4 mL9.4 mL9.4 mL9.4 mL9.4 mL
10% SDS250 µL250 µL250 µL250 µL250 µL250 µL250 µL250 µL
50% Sucrose*4.0 mL4.0 mL4.0 mL4.0 mL4.0 mL4.0 mL4.0 mL4.0 mL
Water7.8 mL6.8 mL5.8 mL4.8 mL3.7 mL2.7 mL1.7 mL750 µL
TEMED**6.25 µL6.25 µL6.25 µL6.25 µL6.25 µL6.25 µL6.25 µL6.25 µL
Catalyst**625 µL625 µL625 µL625 µL625 µL625 µL625 µL625 µL
* Optional, but recommended because it makes it easy to form a good interface between the separating gel and the overlay. If omitted, increase the amount of water added to make up for the volume of the sucrose solution (increase the water by 4.0 mL for the above tables).
** Add these last and mix well just before the gel is to be poured.

Stacking gels

Following recipe is for 4% stacking gels (12.5 mL)

Solution4%
50% Acrylamide/BIS1.0 mL
Stacking Gel Buffer4.2 mL
10% SDS125 µL
Water6.3 mL
TEMED**5.0 µL
Catalyst**1.0 mL

** Add this last and mix well just before the gel is to be poured

 

Bolt Mini Gel Cassettes

Novex Empty Cassettes

Size availableMini Gel: 8x8cm (1.0 mm thick)Mini gel: 8x8 cm (1.5 or 1.0 mm thick)
Midi gel: 8x13 cm (1.0 mm thick)
Equipment requiredMini Gel Tank or XCell SureLock Mini-CellMini Gels: Mini Gel Tank or XCell SureLock Mini-Cell
Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only)
Combs available10 and 12-well Wedgewell (high capacity sample well)1, 2, 2D, 5, 10, 12, 15-well
Product benefits
  • Load up to 60µL per well
  • Sealed on three sides for no gel leaking
  • No casting stand required
  • Special coating provides an uniform polymerization rate for a more consistent handcast gel
  • Sealed on three sides for no gel leaking
  • No casting stand required
  • Special coating provides an uniform polymerization rate for a more consistent handcast gel

Recommended maximum loading volumes

Well Type1.0 mm thickness1.5 mm thickness
10-well Wedgewell60 μL 
12-well Wedgewell45 μL 
1-well700 μL 
5-well60 μL 
10-well25 µL37 µL
12-well20 µL 
15-well15 µL25 µL
2D-well400 µL 
12+2* well45 µL + 15 µL 
20* well25 µL 
26* well15 µL 

* Midi Gel

Gel casting recipes

Choose the polyacrylamide percentage and type of well for your application based upon the expected molecular weight of your protein of interest and the volume of your sample.

Resolve large molecules with low percentage gels and small molecules with high percentage gels. If the molecular weight of the molecule is unknown, or the sample contains a wide range of molecules, use a mid-range percentage gel.

Recipes for SureCast reagents

Prepare resolving gel solution according to the volumes in the following table. The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast.

Note: Solutions do not require degassing.

 Polyacrylamide %
Solution4%6%8%10%12%14%16%18%20%
SureCast Acrylamide (40%)0.8 mL1.2 mL1.6 mL2.0 mL2.4 mL2.8 mL3.3 mL3.6 mL4.0 mL
SureCast Resolving Buffer2.0 mL2.0 mL2.0 mL2.0 mL2.0 mL2.0 mL2.0 mL2.0 mL2.0 mL
Distilled water5.1 mL4.7 mL4.3 mL3.9 mL3.5 mL3.1 mL2.7 mL2.3 mL1.9 mL
10% SureCast APS80 µL80 µL80 µL80 µL80 µL80 µL80 µL80 µL80 µL
SureCast TEMED**8 µL8 µL8 µL8 µL8 µL8 µL8 µL8 µL8 µL

** Add this last and mix well just before the gel is to be poured

Prepare stacking gel solution according to the following table. The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast. Note: Solutions do not require degassing.

Solution4%
SureCast Acrylamide (40%)0.30 mL
SureCast Stacking Buffer0.75 mL
Distilled water1.92 mL
10% SureCast APS30 µL
SureCast TEMED**3 µL
** Add this last and mix well just before the gel is to be poured

Recipes for standalone reagents

Prepare the following stock solutions: All solutions can be stored at room temperature.

50% Acrylamide/BIS (29:1)

  • 48.3 g Acrylamide
  • 1.7 g Bis-acrylamide
Bring to 100 mL with water
Store up to two months in a dark glass bottle

Separating Gel Buffer (1 M Tris-HCl, pH 8.8)

  • Add 30.3 g Tris to 150 mL water
  • Adjust pH 8.8 with HCL
Bring to 250 mL with water

Stacking Gel Buffer (0.375M Tris HCl, pH 6.8)

  • Add 11.4 g Tris to about 150 mL water
  • Adjust to pH 6.8 with HCl
Bring to 250 mL with water

Catalyst- Ammonium Persulfate (Make fresh the day of use)

  • 100 mg Ammonium Persulfate
  • Bring to 2 mL in water

10% SDS

  • 10.0g SDS
Bring to 100 mL with water

50% Sucrose

  • 50.0 g Sucrose
Bring to 100 mL with water
  

Separating gels

The following recipes are for approximately 25 mL of separating gels, enough for four 1.0-mm thick mini gels. Scale volumes proportionally based on the number of gels to be cast.

Solution 6% Gel 8% Gel10% Gel12% Gel14% Gel16% Gel18% Gel20% Gel
50% Acrylamide/BIS3.0 mL4.0 mL5.0 mL6.0 mL7.0 mL8.0 mL9.0 mL10.0 mL
Separating Gel Buffer9.4 mL9.4 mL9.4 mL9.4 mL9.4 mL9.4 mL9.4 mL9.4 mL
10% SDS250 µL250 µL250 µL250 µL250 µL250 µL250 µL250 µL
50% Sucrose*4.0 mL4.0 mL4.0 mL4.0 mL4.0 mL4.0 mL4.0 mL4.0 mL
Water7.8 mL6.8 mL5.8 mL4.8 mL3.7 mL2.7 mL1.7 mL750 µL
TEMED**6.25 µL6.25 µL6.25 µL6.25 µL6.25 µL6.25 µL6.25 µL6.25 µL
Catalyst**625 µL625 µL625 µL625 µL625 µL625 µL625 µL625 µL
* Optional, but recommended because it makes it easy to form a good interface between the separating gel and the overlay. If omitted, increase the amount of water added to make up for the volume of the sucrose solution (increase the water by 4.0 mL for the above tables).
** Add these last and mix well just before the gel is to be poured.

Stacking gels

Following recipe is for 4% stacking gels (12.5 mL)

Solution4%
50% Acrylamide/BIS1.0 mL
Stacking Gel Buffer4.2 mL
10% SDS125 µL
Water6.3 mL
TEMED**5.0 µL
Catalyst**1.0 mL

** Add this last and mix well just before the gel is to be poured

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