Recommended Well Loading Volumes & Sample Loads | Thermo Fisher Scientific

Recommended loading volumes per well for mini gels

	Recommended loading volume*		Maximum protein load per band	DNA per band
Well format	1.0 mm thickness	1.5 mm thickness	Maximum protein load per band	DNA per band
1-well	700 μL	-	12 µg	2.4 µg
5-well	60 μL	-	2 µg	400 ng
9-well	28 μL	-	0.5 µg	100 ng
10-well	25 µL	37 µL	0.5 µg	100 ng
12-well	20 µL	-	0.5 µg	100 ng
15-well	15 µL	25 µL	0.5 µg	100 ng
17-well	15 μL	-	0.5 µg	-
IPG	7 cm IPG strip	-	-	-
2D-well	400 µL	600 μL	12 µg	2.0 µg

* Recommended loading volume represents ~60% of maximum loading volume

Well format	Recommended loading volume	Maximum loading volume	Maximum protein load per band
10-well WedgeWell	40 μL	60 μL	0.5 µg
12-well Wedgewell	30 μL	45 μL	0.4 µg
15-well Wedgewell	20 μL	35 μL	0.25 µg
17-well Wedgewell	15 μL	30 μL	0.2 µg