Recommended loading volumes per well for mini gels

Standard gel combs

 Recommended loading volume*Maximum protein load per bandDNA per band
Well format1.0 mm thickness1.5 mm thickness
1-well700 μL-12 µg2.4 µg
5-well60 μL-2 µg400 ng
9-well28 μL-0.5 µg100 ng
10-well25 µL37 µL0.5 µg100 ng
12-well20 µL-0.5 µg100 ng
15-well15 µL25 µL0.5 µg100 ng
17-well15 μL-0.5 µg-
IPG7 cm IPG strip---
2D-well400 µL600 μL12 µg2.0 µg

* Recommended loading volume represents ~60% of maximum loading volume

WedgeWell combs
(e.g. Bolt Bis-Tris Plus Mini Gels and Novex Tris-Glycine mini precast gels)

Well formatRecommended loading volumeMaximum loading volumeMaximum protein load per band
10-well WedgeWell40 μL60 μL0.5 µg
12-well Wedgewell30 μL45 μL0.4 µg
15-well Wedgewell20 μL35 μL0.25 µg
17-well Wedgewell15 μL30 μL0.2 µg

Recommended loading volumes per well for midi gels

Well formatRecommended loading volume*Maximum loading volumeMaximum protein load per band for silver staining
12+2-well45 μL + 15 μL60.7 μg1.4 ng
20-well25 μL0.5 μg1 ng
26-well15 μL0.35 μg0.7 ng

* Recommended loading volume represents ~60% of maximum loading volume
 

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