Recommended loading volumes per well for mini gels

Standard gel combs

  Recommended loading volume* Maximum protein load per band DNA per band
Well format 1.0 mm thickness 1.5 mm thickness
1-well 700 μL - 12 µg 2.4 µg
5-well 60 μL - 2 µg 400 ng
9-well 28 μL - 0.5 µg 100 ng
10-well 25 µL 37 µL 0.5 µg 100 ng
12-well 20 µL - 0.5 µg 100 ng
15-well 15 µL 25 µL 0.5 µg 100 ng
17-well 15 μL - 0.5 µg -
IPG 7 cm IPG strip - - -
2D-well 400 µL 600 μL 12 µg 2.0 µg

* Recommended loading volume represents ~60% of maximum loading volume

WedgeWell combs
(e.g. Bolt Bis-Tris Plus Mini Gels and Novex Tris-Glycine mini precast gels)

Well format Recommended loading volume Maximum loading volume Maximum protein load per band
10-well WedgeWell 40 μL 60 μL 0.5 µg
12-well Wedgewell 30 μL 45 μL 0.4 µg
15-well Wedgewell 20 μL 35 μL 0.25 µg
17-well Wedgewell 15 μL 30 μL 0.2 µg

Recommended loading volumes per well for midi gels

Well format Recommended loading volume* Maximum loading volume Maximum protein load per band for silver staining
12+2-well 45 μL + 15 μL 60.7 μg 1.4 ng
20-well 25 μL 0.5 μg 1 ng
26-well 15 μL 0.35 μg 0.7 ng

* Recommended loading volume represents ~60% of maximum loading volume

 

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