The Invitrogen NativePAGE Bis-Tris Gel System is a precast polyacrylamide mini-gel system that provides sensitive, high-resolution analysis of native proteins and protein complexes for molecular mass estimations, and assessment of purity. It is based on the blue native polyacrylamide gel electrophoresis (BN PAGE) technique developed by Schägger and von Jagow, which overcomes the limitations of traditional native electrophoresis by providing a near-neutral operating pH and detergent compatibility.
Features of the NativePAGE Bis-Tris Gel System include:
- Wide molecular weight resolving range, from 15 kDa to 10 MDa
- Neutral-pH separation, which better preserves the native state of protein complexes
- Resolution of all proteins in the gel regardless of their isoelectric point (pI)
- Ability to analyze membrane-protein complexes in their native conformations
- Ability to obtain better resolution than with traditional tris-glycine native electrophoresis
How NativePAGE Bis-Tris Gels work
In standard SDS-PAGE, the charge-shift molecule is SDS. The SDS denatures proteins and binds to them, which confers a net negative charge; this allows the proteins to migrate in one direction towards the anode. The SDS is present in the sample buffer and running buffer. In NativePAGE Bis-Tris Gels, the Coomassie G-250 binds to proteins and confers a net negative charge while maintaining the proteins in their native state without any protein denaturation. The G-250 is present in the cathode buffer to provide a continuous flow of G-250 into the gel, and is added to samples containing non-ionic detergent prior to loading the samples onto the gel. The gels do not contain any G-250.
|Available gel sizes||Mini: 8 cm x 8 cm (1 mm thick)|
|Storage conditions||Room temperature|
|Shelf life||6 months|
|Recommended sample buffer||Invitrogen NativePAGE Sample Buffer|
|Recommended running buffers||Invitrogen NativePAGE Running Buffer|
|Available polyacrylamide concentrations||3–12%, 4–16%|
|Separation range||15–10,000 kDa|
|For use with (equipment) mini gels||Mini Gel Tank or XCell SureLock Mini-Cell|
|Mode of separation||Molecular weight|
For Research Use Only. Not for use in diagnostic procedures.