Zymogram gels

Invitrogen Novex Zymogram Gels are excellent tools for detecting and characterizing proteases that utilize casein or gelatin as a substrate.

Interactive selection toolAvailable Novex Zymogram Gels

Migration chart

Novex Zymogram Gels are excellent tools for detecting and characterizing proteases that utilize gelatin as a substrate. Novex Zymogram Gels can be used to analyze a variety of enzymes, including matrix metalloproteinases, lipases, and other proteases. Novex Zymogram Gels are based on tris-glycine gel chemistry containing gelatin as the substrate.

How Novex Zymogram Gels work

Protease samples are denatured in SDS buffer under non-reducing conditions and without heating, and run on a Novex Zymogram Gel using tris-glycine SDS running buffer. After electrophoresis, the enzyme is renatured by incubating the gel in Invitrogen Novex Zymogram Renaturing Buffer, which contains a nonionic detergent. The gels are then equilibrated in Invitrogen Novex Zymogram Developing Buffer to add divalent metal cations required for enzymatic activity, and then stained and destained. Regions of protease activity appear as clear bands against a dark blue background where the protease has digested the substrate.

Available gel sizeMini: 8 cm x 8 cm (1 mm thick) 
Storage conditions2–8°C
Shelf life2 months
Gel chemistry Tris-glycine–containing gelatin or casein
Running bufferTris-glycine SDS running buffer
Available polyacrylamide concentrations10% (w/gelatin)
Separation range10–220 kDa
For use with (equipment) mini gelsMini Gel Tank or XCell SureLock Mini-Cell
Mode of separation Molecular weight
 Zymogram Gelatin Gel
Gel composition10% tris-glycine gel
Substrate0.1% gelatin
Sensitivity5 x 10-6 units of collagenase
Post-staining requiredYes
Separation range20–120 kDa
Migration chart

The numbered bands refer to the following proteases:
Band 1: Collagenase Type I (140 kDa)
Band 2: Thermolysin (37 kDa)
Band 3: Chymotrypsin (30 kDa)
Band 4: Trypsin (19 kDa)

Novex Zymogram Gels are excellent tools for detecting and characterizing proteases that utilize gelatin as a substrate. Novex Zymogram Gels can be used to analyze a variety of enzymes, including matrix metalloproteinases, lipases, and other proteases. Novex Zymogram Gels are based on tris-glycine gel chemistry containing gelatin as the substrate.

How Novex Zymogram Gels work

Protease samples are denatured in SDS buffer under non-reducing conditions and without heating, and run on a Novex Zymogram Gel using tris-glycine SDS running buffer. After electrophoresis, the enzyme is renatured by incubating the gel in Invitrogen Novex Zymogram Renaturing Buffer, which contains a nonionic detergent. The gels are then equilibrated in Invitrogen Novex Zymogram Developing Buffer to add divalent metal cations required for enzymatic activity, and then stained and destained. Regions of protease activity appear as clear bands against a dark blue background where the protease has digested the substrate.

Available gel sizeMini: 8 cm x 8 cm (1 mm thick) 
Storage conditions2–8°C
Shelf life2 months
Gel chemistry Tris-glycine–containing gelatin or casein
Running bufferTris-glycine SDS running buffer
Available polyacrylamide concentrations10% (w/gelatin)
Separation range10–220 kDa
For use with (equipment) mini gelsMini Gel Tank or XCell SureLock Mini-Cell
Mode of separation Molecular weight
 Zymogram Gelatin Gel
Gel composition10% tris-glycine gel
Substrate0.1% gelatin
Sensitivity5 x 10-6 units of collagenase
Post-staining requiredYes
Separation range20–120 kDa
Migration chart

The numbered bands refer to the following proteases:
Band 1: Collagenase Type I (140 kDa)
Band 2: Thermolysin (37 kDa)
Band 3: Chymotrypsin (30 kDa)
Band 4: Trypsin (19 kDa)

Resources