Protein ladders and standards for SDS-PAGE, western blots, and isoelectric focusing (IEF)

Choose from a variety of protein ladders (molecular weight markers) for protein electrophoresis and western blotting applications. Each protein standard or ladder is supplied in a ready-to-use format, eliminating the need to reduce, pre-mix, or add loading dyes. These protein standards and ladders are consistent from lot to lot and are strictly quality controlled on appropriate gels to help ensure consistent band migration and intensity. All prestained protein ladders are compatible for use in western blotting.

Prestained broad and high molecular weight protein ladders

Prestained molecular weight standards are ideal to monitor the progression of the gel electrophoresis run and to estimate protein transfer efficiency. Available in blue and multicolor options, they are compatible with all Invitrogen SDS-PAGE gels. The combination of high molecular weight range protein ladders and NuPAGE Tris-Acetate protein gels is recommended for optimal separation and detection of high molecular weight proteins.

Product description

PageRuler Plus Prestained Protein Ladder

PageRuler Plus Stained Protein Ladder
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Spectra Multicolor Broad Range Protein Ladder

Spectra Multicolor Broad Range Protein Ladder
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HiMark Prestained Protein Standard

HiMark Prestained Protein Standard
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SeeBlue Pre-Stained Standard

SeeBlue Prestained Protein Standard
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Choose forBest multicolor prestained ladder for routine applications4 colors for improved visualization during separation and transferAnalysis of high molecular weight proteinsVisible monitoring of gel separation and transfer efficiency
Range (kDa)10–25010–26031–4603–200
Number of bands91099
Recommended gelAll SDS-PAGE gelsAll SDS-PAGE gelsNuPAGE Tris-acetate

All SDS-PAGE gels

Recommended Volume per well for 1.0 mm gel (μL)

5 µL for electrophoresis

5 µL for blotting

10 µL for electrophoresis

10 µL for blotting

10 µL for electrophoresis

10 µL for blotting

10 µL for electrophoresis

3 µL for blotting

Visualization methodColorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands)Colorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands)ColorimetricColorimetric
Cat. No.26619 (2 x 250 µL), 26620
(10 x 250 µL)
26634 (2 x 250 µL), 26623
(10 x 250 µL)
LC5699 (250 µL)LC5625 (2 x 250 µL)

Protein ladders for western blotting and fluorescent western blotting

Western blot protein ladders are designed for protein molecular weight estimation directly on the blot during detection. Consisting of recombinant proteins fused to an IgG binding site, these versatile molecular weight ladders bind the primary or secondary antibody used for the detection of the target protein. Western blotting ladders provide a useful positive control to evaluate the performance of the detection antibody. Direct visualization of unstained ladders can also be achieved through standard staining protocols (e.g., Coomassie stains).

Product descriptioniBright Prestained Protein Ladder
iBright Prestained Protein Ladder
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MagicMark XP Western Protein Standard
MagicMark XP Western Protein Standard
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Choose forExceptional versatility for visible, IgG or fluorescent western blot detectionIgG binding for direct visualization during detection of western blot
Range (kDa)11–25020–220
Number of bands129
Recommended gelAll SDS-PAGE gelsAll SDS-PAGE gels
Recommended volume per well for 1.0 mm gel (μL)1–35–10
Visualization methodColorimetric, NIR fluorescence (700 nm channel), user defined detection system (2 bands)User defined detection system (not recommended for RGB fluorescence systems)
PropertiesIgG binding sites on 2 bands (80 and 30 kDa), 10 fluorescent, blue-stained bandsIgG binding sites on all bands
Cat. No.LC5615 (2 x 250 µL)LC5602 (250 µL)
LC5603 (50 µL)

Unstained protein ladders

Unstained protein ladders are useful when performing precise molecular weight determination of the target protein. Unstained protein standards are not labeled with dyes that can alter the apparent molecular weight of the ladder in the gel. They can be visualized by direct staining with Coomassie or other protein stains. PageRuler Unstained Protein Standards contain Strep-tagTM II sequence and can be immunodetected on the western blot.

Product descriptionPageRuler Unstained Protein Ladder

PageRuler Unstained Protein Ladder
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PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained High Range Protein Ladder
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HiMark Unstained Protein Standard

HiMark Unstained Protein Standard
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Choose for14 bands for superior accuracyAccurate estimation across a broader rangeAnalysis of high molecular weight proteins
Range (kDa)10–2005–25040–500
Number of Bands14119
Recommended gelAll SDS-PAGE gelsAll SDS-PAGE gelsNuPAGE Tris- Acetate
Recommended Volume per well for 1.0 mm gel (μL)555
Visualization methodstain of choicestain of choicestain of choice
PropertiesTagged—proteins contain an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequenceTagged— proteins in the 10 -250 kDa range contain a Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequenceDirect visualization can be achieved through standard staining protocols (e.g., Coomassie, etc.)
Cat. No.26614 (2 x 250 µL)26630 (2 x 250 µL)LC5688 (250 µL)

Protein ladders for IEF, native PAGE and specialty applications

A selection of protein ladders engineered to provide a reliable reference for protein size determination in native PAGE, pI calibration, detection of His-Tagged, phosphorylated, or glycosylated proteins.

IEF Marker 3-10



IEF Marker 3-10

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NativeMark Unstained Protein Standard

NativeMark Unstained Protein Standard

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E-PAGE SeeBlue Pre-stained Standard

E-PAGE SeeBlue Pre-stained Standard

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BenchMark His-tagged Protein Standard


BenchMark His-tagged Protein Standard
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Peppermint Stick Phosphoprotein Molecular Weight Standards

Peppermint Stick Phosphoprotein Molecular Weight Standards
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CandyCane Glycoprotein Molecular Weight Standards

CandyCane Glycoprotein Molecular Weight Standards
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No. of bands
13851068
Band weights
~10.7, 9.5, 8.3, 8.0, 7.8, 7.4, 6.9, 6.0, 5.3, 5.2, 4.5, 4.2, 3.5~1236, 1048, 720, 480, 242, 146, 66, 20 kDa~261, 173, 97, 42, 21 kDa~160, 120, 80, 60, 50, 40, 30, 20, 15, 10 kDa~116, 66, 45, 23.6, 18, 14.4 kDa~180, 97, 82, 66, 42, 29, 18, 14 kDa
Colors
UnstainedUnstainedBlue, orange, pinkUnstainedUnstainedUnstained
Visualization
ColorimetricColorimetricColorimetricEach protein standard contains a 6X His-tag enabling the detection with the InVision His-tag In-gel StainColorimetric or methods that detect phosphorylated proteins such as Pro-Q Diamond phosphoprotein gel stainsColorimetric or methods that detect glycosylated proteins such as Pro-Q Glycoprotein stain kits
Recommended gel
IEF gelsNative-page gelsE-PAGE gelsAll SDS-PAGE gelsAll SDS-PAGE gelsAll SDS-PAGE gels
Recommended volume per well for 1.0 mm mini gel
5 μL5 μL10 μL for electrophoresis, 5 μL for blotting5 μL for electrophoresis, 2.5–5 μL for blotting1 μL0.5 μL
Cat. No.
3921201
(500 µL)
LC0725
(5 x 50 µL)
LC5700
(500 µL)
LC5606
(125 µL)
P33350 (400 µL) P27167 (40 µL)C21852
(400 µL)

Ordering information

Compare all protein standards and ladders

 Range kDaNo. of BandsRecommended gelVisibleFluorescenceWestern detection
NIRRGB
Prestained Protein Ladders
PageRuler Plus Prestained Protein Ladder10–2509all SDS-PAGE gelsBlue, Orange, Green   
PageRuler Prestained Protein Ladder10–180 10all SDS-PAGE gelsBlue, Orange, Green   
Spectra Multicolor Broad Range Protein Ladder10–260 10all SDS-PAGE gelsBlue, Orange, Green, Pink   
SeeBlue Plus2 Pre-stained Protein Standard 3–20010all SDS-PAGE gelsBlue, Pink, Orange   
Novex Sharp Pre-Stained Protein Standard3.5–260 12all SDS-PAGE gelsBlue, Pink, Orange   
BenchMark Pre-stained Protein Ladder6–180 10all SDS-PAGE gelsBlue, Pink   
HiMark Pre-stained Protein Standard30–460 9NuPAGE Tris-AcetateBlue, Pink   
Spectra Multicolor High Range Protein Ladder40–300 8NuPAGE Tris-AcetateBlue, Orange, Green   
Spectra Multicolor Low Range Protein Ladder1.7–406Novex TricineBlue, Orange, Green   
SeeBlue Pre-Stained Standard3–2009all SDS-PAGE gelsBlue   
iBright Prestained Protein Ladder11–25012all SDS-PAGE gelsBlue  IgG binding sites
PageRuler Prestained NIR Protein Ladder11–25010all SDS-PAGE gelsBlue   
Unstained Protein Ladders
MagicMark XP Western Protein Standard20–2209all SDS-PAGE gelsstain of choice  IgG binding sites
PageRuler Unstained Protein Ladder10–20014all SDS-PAGE gelsstain of choice  Strep-tag™ II sequence 
PageRuler Unstained Broad Range Protein Ladder5–25011all SDS-PAGE gelsstain of choice  Strep-tag™ II sequence 
PageRuler Unstained High Range Protein Ladder60–2508NuPAGE Tris-Acetatestain of choice  Strep-tag™ II sequence 
Mark12 Unstained Standard2.5–20012all SDS-PAGE gelsstain of choice   
BenchMark Protein Ladder10–22015all SDS-PAGE gelsstain of choice   
BenchMark Fluorescent Protein Standard11–1557all SDS-PAGE gelsstain of choice   
Novex Sharp Unstained Protein Standard3.5–26012all SDS-PAGE gelsstain of choice   
SuperSignal Molecular Weight Protein Ladder20–1508all SDS-PAGE gelsstain of choice  IgG binding sites
SuperSignal Enhanced Molecular Weight Protein Ladder20–1508all SDS-PAGE gelsstain of choice  IgG binding sites
Pierce Unstained Protein MW Marker14–1167all SDS-PAGE gelsstain of choice   
HiMark Unstained Protein Standard40–5009NuPAGE Tris-Acetatestain of choice   
PageRuler Unstained Low Range Protein Ladder3.4–1008Novex Tricinestain of choice  Strep-tag™ II sequence 
Resources

For Research Use Only. Not for use in diagnostic procedures.