Protein ladders and standards for SDS-PAGE, western blots, and isoelectric focusing (IEF)
Choose from a variety of protein ladders (molecular weight markers) for protein electrophoresis and western blotting applications. Each protein standard or ladder is supplied in a ready-to-use format, eliminating the need to reduce, pre-mix, or add loading dyes. These protein standards and ladders are consistent from lot to lot and are strictly quality controlled on appropriate gels to help ensure consistent band migration and intensity. All prestained protein ladders are compatible for use in western blotting.
Broad MW range protein ladders
PageRuler Prestained Protein Ladder | PageRuler Plus Prestained Protein Ladder | Spectra Multicolor Broad Range Protein Ladder Click image to enlarge | BenchMark Pre-stained Protein Ladder Click image to enlarge | Novex Sharp Pre-Stained Protein Standard Click image to enlarge | SeeBlue Plus2 Pre-stained Standard Click image to enlarge |
| No. of bands | |||||
| 10 | 9 | 10 | 10 | 12 | 10 |
| Band weights | |||||
| ~180, 130, 100, 70, 55, 40, 35, 25, 15, 10 kDa | ~250, 130, 100, 70, 55, 35, 25, 15, 10 kDa | ~260, 140, 100, 70, 50, 40, 35, 25, 15, 10 kDa | ~180, 115, 82, 64, 49, 37, 26, 19, 15, 6 kDa | ~260, 160, 110, 80, 60, 50, 40, 30, 20, 15, 10, 3.5 kDa | ~198, 98, 62, 49, 38, 28, 17, 14, 6, 3 kDa |
| Colors | |||||
| Blue, orange, green | Blue, orange, green | Orange, pink, blue, green | Blue, pink | Blue, pink, orange | Blue, yellow, pink |
| Visualization | |||||
| Colorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550nm channel, orange bands) | Colorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550nm channel, orange bands) | Colorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550nm channel, orange bands) | Colorimetric | Colorimetric | Colorimetric |
| Recommended gel | |||||
| All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels |
| Recommended volume per well for 1.0 mm mini gel | |||||
| 5 μL for electrophoresis, 5 μL for blotting | 5 μL for electrophoresis, 5 μL for blotting | 10 μL for electrophoresis, 10 μL for blotting | 10 μL for electrophoresis, 5 μL for blotting | 10 μL for electrophoresis, 5 μL for blotting | 10 μL for electrophoresis, 3 μL for blotting |
| Cat. No. | |||||
| 26616 (2 x 250 µL), 26617 (10 x 250 µL) | 26619 (2 x 250 µL), 26620 (10 x 250 µL) | 26634 (2 x 250 µL), 26623 (10 x 250 µL) | 10748010 (500 µL) | LC5800 (2 x 250 µL) | LC5925 (500 µL) |
High MW range protein ladders
HiMark Prestained Protein Standard Click image to enlarge | Spectra Multicolor High Range Protein Ladder Click image to enlarge |
| No. of bands | |
| 9 | 8 |
| Band weights | |
| ~ 460, 268, 238, 171, 117, 71, 55, 41, 31 kDa | ~300, 250, 180, 130, 100, 70, 50, 40 kDa |
| Colors | |
| Purple, pink | Blue, orange, green |
| Visualization | |
| Colorimetric | Colorimetric, NIR fluorescence (700 nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands) |
| Recommended gel | |
| Tris-acetate | Tris-acetate |
| Recommended volume per well for 1.0 mm mini gel | |
| 10 μL for electrophoresis, 10 μL for blotting | 10 μL for electrophoresis, 10 μL for blotting |
| Cat. No. | |
| LC5699 (250 µL) | 26625 (2 x 250 µL) |
Low MW range protein ladders
Spectra Multicolor Low Range Protein Ladder Click image to enlarge | SeeBlue Pre-Stained Standard Click image to enlarge |
| No. of bands | |
| 6 | 9 |
| Band weights | |
| ~40, 25, 15, 10, 4.6, 1.7kDa | 188, 62, 49, 38, 28, 18, 14, 6, 3 kDa |
| Colors | |
| Blue, orange, green | Blue |
| Visualization | |
| Colorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550nm channel, orange bands) | Colorimetric |
| Recommended gel | |
| Tricine | Tricine |
| Recommended volume per well for 1.0 mm mini gel | |
| 5 μL for electrophoresis, 5 μL for blotting | 10 μL for electrophoresis, 3 μL for blotting |
| Cat. No. | |
| 26628 (2 x 250 µL) | LC5625 (500 µL) |
Western blot proteins ladders
Western Blot specific protein ladders are specifically designed for easy and convenient protein molecular weight estimation directly on western blots or indirectly by using various blotting detection systems (e.g. chemiluminescence, fluorescence, or chromogenic). The protein markers consists of recombinant proteins with an IgG binding site. The IgG binding site binds the primary or secondary antibody used for detection of the target protein, allowing visualization of the standard on the western blot. Direct visualization can be achieved through standard staining protocols (e.g. commassise stains), or indirect through chromogenic, chemiluminescent, or fluorescent detection systems.
| Western | Fluorescent | ||||
iBright Prestained Protein Ladder Click image to enlarge | MagicMark XP Western Protein Standard Click image to enlarge | SuperSignal Molecular Weight Protein Ladder Click image to enlarge | SuperSignal Enhanced Molecular Weight Protein Ladder Click image to enlarge | PageRuler Prestained NIR Protein Ladder Click image to enlarge | BenchMark Fluorescent Protein Standard Click image to enlarge |
| No. of bands | |||||
| 12 | 9 | 8 | 8 | 10 | 7 |
| Band weights | |||||
| ~250, 130, 95, 80, 70, 55, 43, 34, 30, 26, 15, 11 kDa | ~220, 120, 100, 80, 60, 50, 40, 30, 20 kDa | ~150, 100, 80, 60, 50, 40, 30, 20 kDa | ~150, 100, 80, 60, 50, 40, 30, 20 kDa | ~250, 130, 95, 70, 55, 43, 34, 26, 15, 11 kDa | ~155, 98, 63, 40, 32, 21, 11 kDa |
| Properties | |||||
| IgG binding sites on 2 bands (80 and 30 kDa), 10 fluor-labeled, blue-stained bands | IgG binding sites on all bands | IgG binding sites on all bands | IgG binding sites on all bands, recommended for mouse monoclonal antibodies or weak binding antibody systems | All bands blue-stained and fluor-labeled | Bands conjugated to Alexa Fluor 488 |
| Visualization | |||||
| Colorimetric, NIR fluorescence (700 nm channel), user defined detection system (2 bands) | User defined detection system (not recommended for RGB fluorescence systems) | User defined detection system(not recommended for RGB fluorescence systems) | User defined detection system(not recommended for RGB fluorescence systems) | Colorimetric, NIR fluorescence (670 nm red laser or 700 nm channels) | UV or RGB fluorescence (488 nm channel) |
| Recommended gel | |||||
| All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels |
| Recommended volume per well for 1.0 mm mini gel | |||||
| 1–3 μL | 5–10 μL | 1–10 μL | 1–10 μL | 1–5 μL | 2–5 μL |
| Cat. No. | |||||
| LC5615 (2 x 250 µL) | LC5602 (250 µL) LC5603 (50 µL) | 84785 (250 µL) | 84786 (250 µL) | 26635 (500 µL) | LC5928 (125 µL) |
High MW range protein ladders
HiMark Unstained Protein Standard Click image to enlarge | PageRuler Unstained High Range Protein Ladder Click image to enlarge | HiMark Prestained protein Standard Click image to enlarge | Spectra Multicolor High Range Protein Ladder Click image to enlarge |
| No. of bands | |||
| 9 | 8 | 9 | 8 |
| Band weights | |||
| ~500, 290, 240, 160, 116, 97, 66, 55, 40 kDa | 250, 200, 150, 120, 100, 85, 70, 60 kDa | ~460, 268, 238, 171, 117, 71, 55, 41, 31 kDa | ~300, 250, 180, 130, 100, 70, 50, 40 kDa |
| Colors | |||
| Unstained | Unstained | Purple, pink | Blue, orange, green |
| Visualization | |||
| User defined staining method | User defined staining method | Colorimetric | Colorimetric, NIR fluorescence (700 nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands) |
| Recommended gel | |||
| Tris-acetate | Tris-acetate | Tris-acetate | Tris-acetate |
| Recommended volume per well for 1.0 mm mini gel | |||
| 5 μL | 5 μL | 10 μL for electrophoresis, 10 μL for blotting | 10 μL for electrophoresis, 10 μL for blotting |
| Cat. No. | |||
| LC5688 (250 µL) | 26637 (2 x 250 µL) | LC5699 (250 µL) | 26625 (2 x 250 µL) |
Low MW range protein ladders
PageRuler Unstained Low Range Protein Ladder Click image to enlarge | Spectra Multicolor Low Range Protein Ladder Click image to enlarge | SeeBlue Pre-Stained Standard Click image to enlarge |
| No. of bands | ||
| 8 | 6 | 9 |
| Band weights | ||
| ~100, 30, 25, 20, 15, 10, 5, 3.4 kDa | ~40, 25, 15, 10, 4.6, 1.7 kDa | 198, 62, 49, 38, 28, 18, 14, 6, 3 kDa |
| Colors | ||
| Unstained | Blue, orange, green | Blue |
| Visualization | ||
| User defined staining method | Colorimetric, NIR fluorescence (700 nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands) | Colorimetric |
| Recommended gel | ||
| Tricine | Tricine | Tricine |
| Recommended volume per well for 1.0 mm mini gel | ||
| 5 μL | 5 μL for electrophoresis, 5 μL for blotting | 10 μL for electrophoresis, 3 μL for blotting |
| Cat. No. | ||
| 26632 (2 x 250 µL) | 26628 (2 x 250 µL) | LC5625 (500 µL) |
Unstained broad MW range protein ladders
PageRuler Unstained Protein Ladder Click image to enlarge | Mark12 Unstained Standard Click image to enlarge | Pierce Unstained Protein MW Marker Click image to enlarge | PageRuler Unstained Broad Range Protein Ladder Click image to enlarge | Novex Sharp Unstained Protein Standard Click image to enlarge | BenchMark Protein Ladder Click image to enlarge |
| No. of bands | |||||
| 14 | 12 | 7 | 11 | 12 | 15 |
| Band weights | |||||
| ~200, 150, 120, 100, 85, 70, 60, 50, 40, 30, 25, 20, 15, 10 kDa | ~200, 116.3, 97.4, 66.3, 55.4, 36.5, 31, 21.5, 14.4, 6, 3.5, 2.5 kDa | ~116, 66.2, 45, 35, 25, 18.4, 14.4 kDa | ~250, 150, 100, 70, 50, 40, 30, 20, 15, 10, 5 kDa | ~260, 160, 110, 80, 60, 50, 40, 30, 20, 15, 10, 3.5 kDa | ~220, 160, 120, 100, 90, 80, 70, 60, 50, 40, 30, 25, 20, 15, 10 kDa |
| Recommended gel | |||||
| All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels |
| Recommended volume per well for 1.0 mm mini gel | |||||
| 5 μL | 5 μL | 5 μL | 5 μL | 5 μL | 5 μL |
| Cat. No. | |||||
| 26614 (2 x 250 µL) | LC5677 (1 mL) | 26610 (2 x 1 ml) | 26630 (2 x 250 µL) | LC5801 (2 x 250 µL) | 10747012 (2 x 250 µL) |
Unstained high and low MW range protein ladders
HiMark Unstained Protein Standard Click image to enlarge | PageRuler Unstained High Range Protein Ladder Click image to enlarge | PageRuler Unstained Low Range Protein Ladder Click image to enlarge |
| No. of bands | ||
| 9 | 8 | 8 |
| Band weights | ||
| ~500, 290, 240, 160, 116, 97, 66, 55, 40 kDa | 250, 200, 150, 120, 100, 85, 70, 60 kDa | ~100, 30, 25, 20, 15, 10, 5, 3.4 kDa |
| Recommended gel | ||
| Tris-acetate | Tris-acetate | Tricine |
| Recommended volume per well for 1.0 mm mini gel | ||
| 5 μL | 5 μL | 5 μL |
| Cat. No. | ||
| LC5688 (250 µL) | 26637 (2 x 250 µL) | 26632 (2 x 250 µL) |
IEF and Specialty Protein Ladders
IEF Marker 3-10 Click image to enlarge | NativeMark Unstained Protein Standard Click image to enlarge | E-PAGE SeeBlue Pre-stained Standard Click image to enlarge | BenchMark His-tagged Protein Standard Click image to enlarge | Peppermint Stick Phosphoprotein Molecular Weight Standards Click image to enlarge | CandyCane Glycoprotein Molecular Weight Standards Click image to enlarge |
| No. of bands | |||||
| 13 | 8 | 5 | 10 | 6 | 8 |
| Band pI | Band weights | ||||
| ~10.7, 9.5, 8.3, 8.0, 7.8, 7.4, 6.9, 6.0, 5.3, 5.2, 4.5, 4.2, 3.5 | ~1236, 1048, 720, 480, 242, 146, 66, 20 kDa | ~261, 173, 97, 42, 21 kDa | ~160, 120, 80, 60, 50, 40, 30, 20, 15, 10 kDa | ~116, 66, 45, 23.6, 18, 14.4 kDa | ~180, 97, 82, 66, 42, 29, 18, 14 kDa |
| Colors | |||||
| Unstained | Unstained | Blue, orange, pink | Unstained | Unstained | Unstained |
| Visualization | |||||
| Colorimetric | Colorimetric | Colorimetric | Each protein standard contains a 6X His-tag enabling the detection with the InVision His-tag In-gel Stain | Colorimetric or methods that detect phosphorylated proteins such as Pro-Q Diamond phosphoprotein gel stains | Colorimetric or methods that detect glycosylated proteins such as Pro-Q Glycoprotein stain kits |
| Recommended gel | |||||
| IEF gels | Native-page gels | E-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels |
| Recommended volume per well for 1.0 mm mini gel | |||||
| 5 μL | 5 μL | 10 μL for electrophoresis, 5 μL for blotting | 5 μL for electrophoresis, 2.5–5 μL for blotting | 1 μL | 0.5 μL |
| Cat. No. | |||||
| 3921201 (500 µL) | LC0725 (5 x 50 µL) | LC5700 (500 µL) | LC5606 (125 µL) | P33350 (400 µL) P27167 (40 µL) | C21852 (400 µL) |
Prestained protein ladders
Unstained protein ladders
Western blot and fluorescent protein ladders
IEF and specialty protein ladders
Broad MW range protein ladders
PageRuler Prestained Protein Ladder | PageRuler Plus Prestained Protein Ladder | Spectra Multicolor Broad Range Protein LadderClick image to enlarge | BenchMark Pre-stained Protein Ladder Click image to enlarge | Novex Sharp Pre-Stained Protein Standard Click image to enlarge | SeeBlue Plus2 Pre-stained Standard Click image to enlarge |
| No. of bands | |||||
| 10 | 9 | 10 | 10 | 12 | 10 |
| Band weights | |||||
| ~180, 130, 100, 70, 55, 40, 35, 25, 15, 10 kDa | ~250, 130, 100, 70, 55, 35, 25, 15, 10 kDa | ~260, 140, 100, 70, 50, 40, 35, 25, 15, 10 kDa | ~180, 115, 82, 64, 49, 37, 26, 19, 15, 6 kDa | ~260, 160, 110, 80, 60, 50, 40, 30, 20, 15, 10, 3.5 kDa | ~198, 98, 62, 49, 38, 28, 17, 14, 6, 3 kDa |
| Colors | |||||
| Blue, orange, green | Blue, orange, green | Orange, pink, blue, green | Blue, pink | Blue, pink, orange | Blue, yellow, pink |
| Visualization | |||||
| Colorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550nm channel, orange bands) | Colorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550nm channel, orange bands) | Colorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550nm channel, orange bands) | Colorimetric | Colorimetric | Colorimetric |
| Recommended gel | |||||
| All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels |
| Recommended volume per well for 1.0 mm mini gel | |||||
| 5 μL for electrophoresis, 5 μL for blotting | 5 μL for electrophoresis, 5 μL for blotting | 10 μL for electrophoresis, 10 μL for blotting | 10 μL for electrophoresis, 5 μL for blotting | 10 μL for electrophoresis, 5 μL for blotting | 10 μL for electrophoresis, 3 μL for blotting |
| Cat. No. | |||||
| 26616 (2 x 250 µL), 26617 (10 x 250 µL) | 26619 (2 x 250 µL), 26620 (10 x 250 µL) | 26634 (2 x 250 µL), 26623 (10 x 250 µL) | 10748010 (500 µL) | LC5800 (2 x 250 µL) | LC5925 (500 µL) |
High MW range protein ladders
| HiMark Prestained Protein Standard Click image to enlarge | Spectra Multicolor High Range Protein Ladder Click image to enlarge |
| No. of bands | |
| 9 | 8 |
| Band weights | |
| ~ 460, 268, 238, 171, 117, 71, 55, 41, 31 kDa | ~300, 250, 180, 130, 100, 70, 50, 40 kDa |
| Colors | |
| Purple, pink | Blue, orange, green |
| Visualization | |
| Colorimetric | Colorimetric, NIR fluorescence (700 nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands) |
| Recommended gel | |
| Tris-acetate | Tris-acetate |
| Recommended volume per well for 1.0 mm mini gel | |
| 10 μL for electrophoresis, 10 μL for blotting | 10 μL for electrophoresis, 10 μL for blotting |
| Cat. No. | |
| LC5699 (250 µL) | 26625 (2 x 250 µL) |
Low MW range protein ladders
| Spectra Multicolor Low Range Protein Ladder Click image to enlarge | SeeBlue Pre-Stained Standard Click image to enlarge |
| No. of bands | |
| 6 | 9 |
| Band weights | |
| ~40, 25, 15, 10, 4.6, 1.7kDa | 188, 62, 49, 38, 28, 18, 14, 6, 3 kDa |
| Colors | |
| Blue, orange, green | Blue |
| Visualization | |
| Colorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550nm channel, orange bands) | Colorimetric |
| Recommended gel | |
| Tricine | Tricine |
| Recommended volume per well for 1.0 mm mini gel | |
| 5 μL for electrophoresis, 5 μL for blotting | 10 μL for electrophoresis, 3 μL for blotting |
| Cat. No. | |
| 26628 (2 x 250 µL) | LC5625 (500 µL) |
Western blot proteins ladders
Western Blot specific protein ladders are specifically designed for easy and convenient protein molecular weight estimation directly on western blots or indirectly by using various blotting detection systems (e.g. chemiluminescence, fluorescence, or chromogenic). The protein markers consists of recombinant proteins with an IgG binding site. The IgG binding site binds the primary or secondary antibody used for detection of the target protein, allowing visualization of the standard on the western blot. Direct visualization can be achieved through standard staining protocols (e.g. commassise stains), or indirect through chromogenic, chemiluminescent, or fluorescent detection systems.
| Western | Fluorescent | ||||
| iBright Prestained Protein LadderClick image to enlarge | MagicMark XP Western Protein StandardClick image to enlarge | SuperSignal Molecular Weight Protein LadderClick image to enlarge | SuperSignal Enhanced Molecular Weight Protein LadderClick image to enlarge | PageRuler Prestained NIR Protein Ladder Click image to enlarge | BenchMark Fluorescent Protein Standard Click image to enlarge |
| No. of bands | |||||
| 12 | 9 | 8 | 8 | 10 | 7 |
| Band weights | |||||
| ~250, 130, 95, 80, 70, 55, 43, 34, 30, 26, 15, 11 kDa | ~220, 120, 100, 80, 60, 50, 40, 30, 20 kDa | ~150, 100, 80, 60, 50, 40, 30, 20 kDa | ~150, 100, 80, 60, 50, 40, 30, 20 kDa | ~250, 130, 95, 70, 55, 43, 34, 26, 15, 11 kDa | ~155, 98, 63, 40, 32, 21, 11 kDa |
| Properties | |||||
| IgG binding sites on 2 bands (80 and 30 kDa), 10 fluor-labeled, blue-stained bands | IgG binding sites on all bands | IgG binding sites on all bands | IgG binding sites on all bands, recommended for mouse monoclonal antibodies or weak binding antibody systems | All bands blue-stained and fluor-labeled | Bands conjugated to Alexa Fluor 488 |
| Visualization | |||||
| Colorimetric, NIR fluorescence (700 nm channel), user defined detection system (2 bands) | User defined detection system (not recommended for RGB fluorescence systems) | User defined detection system(not recommended for RGB fluorescence systems) | User defined detection system(not recommended for RGB fluorescence systems) | Colorimetric, NIR fluorescence (670 nm red laser or 700 nm channels) | UV or RGB fluorescence (488 nm channel) |
| Recommended gel | |||||
| All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels |
| Recommended volume per well for 1.0 mm mini gel | |||||
| 1–3 μL | 5–10 μL | 1–10 μL | 1–10 μL | 1–5 μL | 2–5 μL |
| Cat. No. | |||||
| LC5615 (2 x 250 µL) | LC5602 (250 µL) LC5603 (50 µL) | 84785 (250 µL) | 84786 (250 µL) | 26635 (500 µL) | LC5928 (125 µL) |
High MW range protein ladders
| HiMark Unstained Protein Standard Click image to enlarge | PageRuler Unstained High Range Protein Ladder Click image to enlarge | HiMark Prestained protein Standard Click image to enlarge | Spectra Multicolor High Range Protein Ladder Click image to enlarge |
| No. of bands | |||
| 9 | 8 | 9 | 8 |
| Band weights | |||
| ~500, 290, 240, 160, 116, 97, 66, 55, 40 kDa | 250, 200, 150, 120, 100, 85, 70, 60 kDa | ~460, 268, 238, 171, 117, 71, 55, 41, 31 kDa | ~300, 250, 180, 130, 100, 70, 50, 40 kDa |
| Colors | |||
| Unstained | Unstained | Purple, pink | Blue, orange, green |
| Visualization | |||
| User defined staining method | User defined staining method | Colorimetric | Colorimetric, NIR fluorescence (700 nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands) |
| Recommended gel | |||
| Tris-acetate | Tris-acetate | Tris-acetate | Tris-acetate |
| Recommended volume per well for 1.0 mm mini gel | |||
| 5 μL | 5 μL | 10 μL for electrophoresis, 10 μL for blotting | 10 μL for electrophoresis, 10 μL for blotting |
| Cat. No. | |||
| LC5688 (250 µL) | 26637 (2 x 250 µL) | LC5699 (250 µL) | 26625 (2 x 250 µL) |
Low MW range protein ladders
| PageRuler Unstained Low Range Protein Ladder Click image to enlarge | Spectra Multicolor Low Range Protein Ladder Click image to enlarge | SeeBlue Pre-Stained Standard Click image to enlarge |
| No. of bands | ||
| 8 | 6 | 9 |
| Band weights | ||
| ~100, 30, 25, 20, 15, 10, 5, 3.4 kDa | ~40, 25, 15, 10, 4.6, 1.7 kDa | 198, 62, 49, 38, 28, 18, 14, 6, 3 kDa |
| Colors | ||
| Unstained | Blue, orange, green | Blue |
| Visualization | ||
| User defined staining method | Colorimetric, NIR fluorescence (700 nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands) | Colorimetric |
| Recommended gel | ||
| Tricine | Tricine | Tricine |
| Recommended volume per well for 1.0 mm mini gel | ||
| 5 μL | 5 μL for electrophoresis, 5 μL for blotting | 10 μL for electrophoresis, 3 μL for blotting |
| Cat. No. | ||
| 26632 (2 x 250 µL) | 26628 (2 x 250 µL) | LC5625 (500 µL) |
Unstained broad MW range protein ladders
| PageRuler Unstained Protein Ladder Click image to enlarge | Mark12 Unstained Standard Click image to enlarge | Pierce Unstained Protein MW Marker Click image to enlarge | PageRuler Unstained Broad Range Protein Ladder Click image to enlarge | Novex Sharp Unstained Protein Standard Click image to enlarge | BenchMark Protein Ladder Click image to enlarge |
| No. of bands | |||||
| 14 | 12 | 7 | 11 | 12 | 15 |
| Band weights | |||||
| ~200, 150, 120, 100, 85, 70, 60, 50, 40, 30, 25, 20, 15, 10 kDa | ~200, 116.3, 97.4, 66.3, 55.4, 36.5, 31, 21.5, 14.4, 6, 3.5, 2.5 kDa | ~116, 66.2, 45, 35, 25, 18.4, 14.4 kDa | ~250, 150, 100, 70, 50, 40, 30, 20, 15, 10, 5 kDa | ~260, 160, 110, 80, 60, 50, 40, 30, 20, 15, 10, 3.5 kDa | ~220, 160, 120, 100, 90, 80, 70, 60, 50, 40, 30, 25, 20, 15, 10 kDa |
| Recommended gel | |||||
| All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels |
| Recommended volume per well for 1.0 mm mini gel | |||||
| 5 μL | 5 μL | 5 μL | 5 μL | 5 μL | 5 μL |
| Cat. No. | |||||
| 26614 (2 x 250 µL) | LC5677 (1 mL) | 26610 (2 x 1 ml) | 26630 (2 x 250 µL) | LC5801 (2 x 250 µL) | 10747012 (2 x 250 µL) |
Unstained high and low MW range protein ladders
| HiMark Unstained Protein Standard Click image to enlarge | PageRuler Unstained High Range Protein Ladder Click image to enlarge | PageRuler Unstained Low Range Protein Ladder Click image to enlarge |
| No. of bands | ||
| 9 | 8 | 8 |
| Band weights | ||
| ~500, 290, 240, 160, 116, 97, 66, 55, 40 kDa | 250, 200, 150, 120, 100, 85, 70, 60 kDa | ~100, 30, 25, 20, 15, 10, 5, 3.4 kDa |
| Recommended gel | ||
| Tris-acetate | Tris-acetate | Tricine |
| Recommended volume per well for 1.0 mm mini gel | ||
| 5 μL | 5 μL | 5 μL |
| Cat. No. | ||
| LC5688 (250 µL) | 26637 (2 x 250 µL) | 26632 (2 x 250 µL) |
IEF and Specialty Protein Ladders
| IEF Marker 3-10 Click image to enlarge | NativeMark Unstained Protein Standard Click image to enlarge | E-PAGE SeeBlue Pre-stained Standard Click image to enlarge | BenchMark His-tagged Protein Standard Click image to enlarge | Peppermint Stick Phosphoprotein Molecular Weight Standards Click image to enlarge | CandyCane Glycoprotein Molecular Weight Standards Click image to enlarge |
| No. of bands | |||||
| 13 | 8 | 5 | 10 | 6 | 8 |
| Band pI | Band weights | ||||
| ~10.7, 9.5, 8.3, 8.0, 7.8, 7.4, 6.9, 6.0, 5.3, 5.2, 4.5, 4.2, 3.5 | ~1236, 1048, 720, 480, 242, 146, 66, 20 kDa | ~261, 173, 97, 42, 21 kDa | ~160, 120, 80, 60, 50, 40, 30, 20, 15, 10 kDa | ~116, 66, 45, 23.6, 18, 14.4 kDa | ~180, 97, 82, 66, 42, 29, 18, 14 kDa |
| Colors | |||||
| Unstained | Unstained | Blue, orange, pink | Unstained | Unstained | Unstained |
| Visualization | |||||
| Colorimetric | Colorimetric | Colorimetric | Each protein standard contains a 6X His-tag enabling the detection with the InVision His-tag In-gel Stain | Colorimetric or methods that detect phosphorylated proteins such as Pro-Q Diamond phosphoprotein gel stains | Colorimetric or methods that detect glycosylated proteins such as Pro-Q Glycoprotein stain kits |
| Recommended gel | |||||
| IEF gels | Native-page gels | E-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels |
| Recommended volume per well for 1.0 mm mini gel | |||||
| 5 μL | 5 μL | 10 μL for electrophoresis, 5 μL for blotting | 5 μL for electrophoresis, 2.5–5 μL for blotting | 1 μL | 0.5 μL |
| Cat. No. | |||||
| 3921201 (500 µL) | LC0725 (5 x 50 µL) | LC5700 (500 µL) | LC5606 (125 µL) | P33350 (400 µL) P27167 (40 µL) | C21852 (400 µL) |
Prestained protein ladders
Unstained protein ladders
Western blot and fluorescent protein ladders
IEF and specialty protein ladders
Resources
For Research Use Only. Not for use in diagnostic procedures.
