View all available protein ladders

Find the right protein ladder for your application and gel system. Use the interactive tool below to see our recommended protein ladders migration in the different gel chemistries.

Why the molecular weight changes between the different gel chemistries?

Slight differences in protein mobilities will occur when the same proteins are run in different SDS-PAGE buffer systems (e.g. Bis-Tris vs Tris-glycine). Each SDS-PAGE buffer system has a different pH, which affects the charge of a protein and its binding capacity for SDS. The degree of change in protein mobility is usually small in natural proteins but is more pronounced with atypical or chemically modified proteins, such as pre-stained standards. Apparent molecular weight values for pre-stained standards will vary between gel systems- it is important to use the apparent molecular weights that matches your gel for the most accurate calibration of your sample proteins.




Recommended uses

  • Precise determination of target protein molecular weight
  • Approximate determination of molecular weight
  • Monitoring the progress of electrophoresis runs
  • Estimating the efficiency of protein transfer to the membrane during western blotting


Protein Gels

Explore: Protein Gels

Protein Gel Guide
Western Detection Workflow brochure
Protein Electrophoresis and Western Blotting Education Center

For Research Use Only. Not for use in diagnostic procedures.