Biotinylation

Biotinylation reagents and kits

We offer a wide range of biotin and desthiobiotin labeling reagents for targeting specific functional groups or residues, including primary amines and sulfhydryls on proteins, peptides, antibodies and other biomolecules. The strong biotin-avidin interaction is exploited to detect and/or purify proteins because of its high specificity.

Use our Biotinylation Reagent Selection Tool to find the optimal labeling reagent for your application, or access our helpful Bioconjugation Technical Handbook to help improve your biotin labeling results. Explore our Biotin Quantitation Reagents and HABA Calculator Tool to measure the amount of biotin attached biomolecule of interest.

Choose the right biotinylation reagent for your experiment based on reactive chemistry

Order products Spacer Arm (Å) Cleavable? Water-soluble? Membrane permeable?
EZ-Link NHS-Biotin 13.5 No No Yes
EZ-Link NHS-Desthiobiotin 9.7 No No Yes
EZ-Link Sulfo-NHS-Biotin 13.5 No Yes No
EZ-Link NHS-LC-Biotin 22.4 No No Yes
EZ-Link Sulfo-NHS-LC-Desthiobiotin, No-Weigh Format 17.3 No Yes No
EZ-Link Sulfo-NHS-LC-Biotin 22.4 No Yes No
EZ-Link NHS-LC-LC-Biotin 30.5 No No Yes
EZ-Link Sulfo-NHS-LC-LC-Biotin 30.5 No Yes No
EZ-Link NHS-SS-Biotin 24.3 Yes No Yes
EZ-Link Sulfo-NHS-SS-Biotin 24.3 Yes Yes No
EZ-Link NHS-PEG4-Biotin 29 No Yes No
EZ-Link NHS-SS-PEG4-Biotin 37.9 Yes Yes Yes
EZ-Link PFP-Biotin 9.5 No No Yes
Labeling scale Order products Spacer Arm (Å) Cleavable? Water-soluble? Membrane permeable?
1-10 mg* EZ-Link NHS-PEG4 Biotinylation Kit 29 No Yes No
EZ-Link Sulfo-NHS-LC-Biotinylation Kit 22.4 No Yes No
EZ-Link Sulfo NHS-SS Biotinylation Kit 24.3 Yes Yes No
EZ-Link Sulfo-NHS-Biotinylation Kit 13.5 No Yes No
50-200 μg EZ-Link Micro NHS-PEG4-Biotinylation Kit 29 No Yes No
EZ-Link Micro Sulfo-NHS-LC-Biotinylation Kit 22.4 No Yes No
EZ-Link Micro Sulfo-NHS-SS-Biotinylation Kit 24.3 Yes Yes No
EZ-Link Micro Sulfo-NHS-Biotinylation Kit 13.5 No Yes No

*1-10 mg biotinylation kits include reagents for a HABA assay for measuring the level of biotin incorporation

Order products Spacer Arm (Å) Cleavable? Water-soluble? Membrane permeable?
EZ-Link BMCC-Biotin 32.6 No No Yes
EZ-Link HPDP-Biotin  29.2 Yes No Yes
EZ-Link Iodoacetyl-LC-Biotin 27.1 No No Yes
EZ-Link Iodoacetyl-PEG2-Biotin 24.7 No Yes No
EZ-Link Maleimide-PEG2-Biotin, 
No-Weigh Format
29.1 No Yes No
EZ-Link Maleimide PEG11-Biotin 59.1 No Yes No
EZ-Link NHS-PEG4-Biotin 29 No Yes No
Order products Spacer Arm (Å) Cleavable? Water-soluble? Membrane permeable?
EZ-Link Amine-PEG2-Biotin 20.4 No Yes No
EZ-Link Amine-PEG3-Biotin 22.9 No Yes No
EZ-Link Amine-PEG11-Biotin 53.2 No Yes No
EZ-Link Biocytin 20.1 No Yes No
EZ-Link Pentylamine-Biotin 18.9 No Yes   No 
EZ-Link Biocytin 20.1 No Yes No
Oder products Spacer Arm (Å) Cleavable? Water-soluble? Membrane permeable?
EZ-Link Alkoxyamine-PEG12-Biotin 55.4 No Yes No
EZ-Link Alkoxyamine-PEG4-Biotin 27 No Yes   No 
EZ-Link Hydrazide-Biocytin 19.7 No Yes Yes
EZ-Link Hydrazide-Biotin 15.7 No Yes Yes 
EZ-Link Biotin-LC-Hydrazide 24.7 No No Yes
EZ-Link Hydrazide-PEG4-Biotin 31.3 No Yes No
Order products Spacer Arm (Å) Cleavable? Water-soluble? Membrane permeable?
EZ-Link Psoralen-PEG3-Biotin 39.6 No   Yes   No
EZ-Link TFPA-PEG3-Biotin 33.4 No No  Yes

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Need larger quantities of our protein labeling, crosslinking or modification reagents? We offer bulk and custom fill sizes.

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