Biotinylation reagents and kits

We offer a wide range of biotin and desthiobiotin labeling reagents for targeting specific functional groups or residues, including primary amines and sulfhydryls on proteins, peptides, antibodies and other biomolecules. The strong biotin-avidin interaction is exploited to detect and/or purify proteins because of its high specificity.

Use our Biotinylation Reagent Selection Tool to find the optimal labeling reagent for your application, or access our helpful Bioconjugation Technical Handbook to help improve your biotin labeling results. Explore our Biotin Quantitation Reagents and HABA Calculator Tool to measure the amount of biotin attached biomolecule of interest.

Choose the right biotinylation reagent for your experiment based on reactive chemistry

*1-10 mg biotinylation kits include reagents for a HABA assay for measuring the level of biotin incorporation

Order productsSpacer Arm (Å)Cleavable?Water-soluble?Membrane permeable?
EZ-Link BMCC-Biotin32.6NoNoYes
EZ-Link HPDP-Biotin 29.2Yes NoYes
EZ-Link Iodoacetyl-LC-Biotin27.1NoNoYes
EZ-Link Iodoacetyl-PEG2-Biotin24.7NoYesNo
EZ-Link Maleimide-PEG2-Biotin, 
No-Weigh Format
EZ-Link Maleimide PEG11-Biotin59.1NoYesNo
EZ-Link NHS-PEG4-Biotin29NoYesNo
Order productsSpacer Arm (Å)Cleavable?Water-soluble?Membrane permeable?
EZ-Link Amine-PEG2-Biotin20.4No Yes No
EZ-Link Amine-PEG3-Biotin22.9No Yes No
EZ-Link Amine-PEG11-Biotin53.2No Yes No
EZ-Link Biocytin20.1No Yes No
EZ-Link Pentylamine-Biotin18.9No Yes  No 
EZ-Link Biocytin20.1NoYesNo
Oder productsSpacer Arm (Å)Cleavable?Water-soluble?Membrane permeable?
EZ-Link Alkoxyamine-PEG12-Biotin55.4No Yes No
EZ-Link Alkoxyamine-PEG4-Biotin27No Yes  No 
EZ-Link Hydrazide-Biocytin19.7NoYes Yes
EZ-Link Hydrazide-Biotin15.7NoYes Yes 
EZ-Link Biotin-LC-Hydrazide24.7NoNoYes
EZ-Link Hydrazide-PEG4-Biotin31.3No Yes No
Order productsSpacer Arm (Å)Cleavable?Water-soluble?Membrane permeable?
EZ-Link Psoralen-PEG3-Biotin39.6 No  Yes  No
EZ-Link TFPA-PEG3-Biotin33.4NoNo Yes
Order productsReactive MoietyCleavable?Water-soluble?
EZ-Link Biotin-PEG12-DBCODBCO (alkyne)NoNo
Biotin AzideAzideNoNo
Biotin AlkyneAlkyneNoNo

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Bulk or custom product request

Need larger quantities of our protein labeling, crosslinking or modification reagents? We offer bulk and custom fill sizes.

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