Our amine-to-amine crosslinker is a homobifunctional amine-specific protein crosslinking reagent that uses NHS-ester and imidoester reactive groups for selective conjugation of primary amines. Our amine-to-amine crosslinkers are available in short, long, cleavable, irreversible, membrane permeable, and cell surface varieties.
Our amine reactive crosslinkers have a variety of useful characteristics:
- Amine-specific crosslinking reagents based on N-hydroxysuccinimide ester (NHS ester) reactive chemistry
- NHS esterreactive groups are formed by EDC-activation of carboxylate molecules
- NHS ester-activated crosslinkers and labeling compounds react with primary amines in slightly alkaline conditions (pH 7.2-8.5) to yield stable amide bonds
- Traditional amine-reactive crosslinking reagents based on the imidoester reactive group
- Imidoester crosslinkers react with primary amines to form amidine bonds
- Best when used in amine-free, alkaline conditions (pH 10), such as with borate buffer, to provide specificity for primary amines
Other amine-reactive crosslinkers
- We offer specialized crosslinking reagents and kits for amine-specific molecular conjugation and polymerization based on phosphine, aldehyde, or aryl halide reactive groups.
|Reactive Group||Products||Spacer Arm (Å)||Cleavable?||Water-soluble?||Membrane-permeable?||Reversible?|
We also offer Molecular Probes® N-(t-BOC)-Aminooxyacetic Acid, Tetrafluorophenyl Ester, which is an amine-reactive protected hydroxylamine that is useful for synthesizing new aldehyde- and ketone-reactive probes in an organic solvent. Following coupling to aliphatic amines, the t-BOC group can be quantitatively removed with trifluoroacetic acid.
For Research Use Only. Not for use in diagnostic procedures.