DCC, EDC, EDAC, NHS and Sulfo-NHS crosslinkers

Carbodiimide crosslinking reagents, DCC and EDC (EDAC), are used for the conjugation of carboxyl groups (glutamate, aspartate, C-termini) to primary amines (lysine, N-termini) and N-hydroxysuccinimide (NHS) for stable activation of carboxylates for amine-conjugation.

A variety of carboxyl-to-amine crosslinkers are available:

  • DCC is water-insoluble carbodiimide-based crosslinker that activates carboxyl groups for spontaneous peptide-bond formation with primary amines.
  • EDC is a water-soluble carbodiimide-based crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation.
  • NHS and sulfo-NHS are chemical modification reagents for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling, and immobilization.

Choose the right carbodiimide and NHS reagent for your experiment

Products Cleavable ? Water-soluble? Membrane permeable?
DCC No No Yes
EDC No Yes No
NHS   No No Yes
Sulfo-NHS No Yes No
Protein quantitation immunoassays
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