Carbonyl Biotinylation Reagents
The carbohydrate portion of glycoproteins is one of the most common targets for protein modification. It can be reacted with hydrazide derivatives of biotin. This approach is useful for antibodies because they become biotinylated in a manner that maintains immunological reactivity; particularly polyclonal antibodies because they are heavily glycosylated. Monoclonal antibodies may be deficient in glycosylation and success with this method will depend on the extent of glycosylation for a particular antibody.
Choose the best carbonyl biotinylation reagent for your experiment
|Reagent category||Order products||Product features (description)||Spacer arm (Å)||Water soluble?||Cleavable?||Membrane permeable?|
|Alkoxyamine-PEG12-Biotin||Glycoprotein biotinylation via a very long polyethylene glycol (PEG) spacer arm
|Alkoxyamine-PEG4-Biotin||Glycoprotein biotinylation via a long polyethylene glycol (PEG) spacer arm||31.3||Yes||No||No|
|Alkoxyamine-PEG4-SS-PEG4-Biotin||Glycoprotein biotinylation via a cleavable pegylated spacer arm||56.6||Yes||Yes||No|
|Hydrazide-Biocytin||Can be used to label DNA and RNA through cytosine residues||19.7||No||No||No|
|Hydrazide-Biotin Reagents||Short and mid-length reagents for labeling glycoproteins and other carbohydrate-containing compounds having oxidizable sugars or aldehydes
|Hydrazide-Biotin Reagents||Short and mid-length reagents for labeling glycoproteins and other carbohydrate-containing compounds having oxidizable sugars or aldehydes||24.7||No||No||Yes|
|Hydrazide-PEG4-Biotin||A carbohydrate-biotinylation reagent with a long, hydrophilic spacer arm||31.3||Yes||No||No|
For Research Use Only. Not for use in diagnostic procedures.