Staudinger Reagent Selection Guide
Choose an appropriate reagent for metabolic labeling and chemoselective ligation.
Thermo Scientific™ Azide and Phosphine Reagents are chemical or metabolic labeling reagents for in vivo or in vitro chemoselective ligation of proteins and post-translational modification moieties. The azide-phosphine crosslinking strategy is called Staudinger ligation and makes possible a variety of specific detection, tagging and affinity purification approaches for studying cellular responses and pathways.
Featured data and reactions
Figure 1. Staudinger chemoselective ligation as a strategy for metabolic labeling. Phosphine-activated reagents (e.g., fluorescent dyes) will specifically conjugate to azide-labeled biomolecules to allow specific detection, purification and measurement.
Figure 2. Detection of metabolically incorporated azido-mannosamine with Thermo Scientific DyLight 650-Phosphine. HK-2 cells were incubated with N-azido-acetyl-mannosamine and the live cells were probed with azide-reactive DyLight 650-Phosphine (blue). Nuclei were counterstained with Hoechst33342 (red).
Highlights, features, and benefits
- Easy-to-use—two-component reaction with no additional accessory reagents required
- Chemoselective—specific reactivity of azide and phosphine minimizes off-target effects and produces very low background
- Bioorthogonal—azido sugars are naturally incorporated without affecting biological activity
- Copper-free—unlike other chemoselective ligation chemistries, Staudinger ligation is non-toxic to live cells and does not oxidize amino acid side chains or post-translational modifications
|Compatible with bioorthogonal metabolic labeling||
|High reaction efficiency||
|Ease of use||
|Allows probe switching||
Quick-link table of products
|Azide (N3) products||Phosphine (P3) products|
|Reagents for chemical activation and conjugation: Crosslinking
|Reagents for metabolic labeling and detection: Labeling
For Research Use Only. Not for use in diagnostic procedures.