We offer purified and agarose-immobilized proteases for enzymatic proteolysis (cleavage or digestion) of proteins to facilitate amino acid sequencing, peptide analysis, and polypeptide structural characterization.

Protease and protein-cleaving reagents selection chart

Enzyme Purified proteases & protein-cleaving reagents Immobilized proteases Specificity
Trypsin, MS Grade 90057
90058
90059
90305
N/A Cleaves at the carboxyl side of arginine and lysine residues
Lys-C Protease, MS Grade 90051 N/A Cleaves at the carboxyl side of lysine residues
Lys-N Protease, MS Grade 90300
90301
N/A Cleaves at the amino side of lysine residues
Asp-N Protease, MS Grade 90053 N/A Cleaves at the amino side of aspartate residues
Chymotrypsin, MS Grade 90056 N/A Cleaves at the carboxyl side of tyrosine, phenylalanine, tryptophan, and leucine
Glu-C Protease, MS Grade 90054 N/A Cleaves at the carboxyl side of glutamate or aspartate
Carboxypeptidase Y 20212 20236 Cleaves residues sequentially from the carboxy terminus
Factor Xa 32521
32520
N/A Cleaves at the carboxyl side of Ile-Glu-Gly-Arg sequence
Human Rhinovirus 3C Protease
(HRV 3C)
88946 Cleaves fusion tags from
recombinant proteins
with this protease that is dual-tagged for easy removal after cleavage.
N/A
Ficin N/A 44881 Cleaves non-specifically at exposed residues, favors aromatic residues
Papain N/A 20341 Cleaves nonspecifically at exposed residues
Pepsin N/A 20343 Cleaves nonspecifically at exposed residues, favors aromatic residues
Proteinase K 17916 N/A Cleaves at the carboxyl side of aliphatic, aromatic, and hydrophobic residues
S. aureus V-8 Protease 20195 20151 Cleaves at the carboxyl side of aspartate and glutamate residues
Trypsin (TPCK) 20233 20230 Cleaves at the carboxyl side of arginine and lysine residues

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