Protein Quantitation Using Mass Spectrometry

• Powerful—isobaric tags and metabolic amino acid labeling offer concurrent MS analysis of multiple samples, to help increase sample throughput and enabling relative quantitation of 2 to 11 different samples derived from cells, tissues, or biological fluids
• Accurate—HeavyPeptide AQUA Custom peptides and SureQuant targeted mass spec assays provide concentration precision to meet your application needs quantitation of proteins at very low concentrations in complex protein mixtures
• Broad portfolio—extensive offering of both kits and individual reagents for discovery and targeted Mass Spec Protein Quantitation
• Compatible—optimized for use with Thermo Scientific LC-MS or LC-MS/MS platforms with data analysis fully supported by Proteome Discoverer 3.1 or Pinpoint 1.4

Discovery vs. targeted quantitation

Discovery proteomics maximizes protein identification by spending more time and effort per sample and reducing the number of samples analyzed. In contrast, targeted proteomics strategies limit the number of features that will be monitored, and then optimize the chromatography, instrument tuning and acquisition methods to achieve the highest sensitivity and throughput for larger sample sets.

Rapid, cell-free expression of recombinant proteins containing stable isotope-labeled (i.e., heavy) amino acids can be generated using the 1-Step Heavy Protein IVT Kit. Heavy proteins expressed using this system can be used as mass spectrometry controls for sample preparation loss, digestion efficiency determination or as quantification standards. The Pierce Peptide Retention Time Calibration Mixture contains 15 synthetic heavy peptides mixed at an equimolar ratio that elute across the chromatographic gradient and can be used to assess LC performance as well to predict peptide retention times in targeted assay development.