Overview of protein quantitation reagents

Mass spectrometry can detect very low analyte concentrations in complex mixtures. It can be quantitative with the use of peptide labels or standards that are concomitantly analyzed with the sample and act as a reference point for both relative or absolute analyte quantitation, respectively. We offer three different systems that can be applied to solve a wide variety of biological problems. Relative quantitation strategies include tandem mass tagging (TMT) and stable isotope labeling using amino acids in cell culture (SILAC). For both relative and absolute quantitation, SureQuant assays are designed for multiplexed targeted quantitation for key cellular signaling proteins.

We offer individual reagents and complete kits for both discovery and targeted quantitative proteomic applications.

Benefits of Mass spectrometry reagents:

  • Powerful—isobaric tags and metabolic amino acid labeling offer concurrent mass spectrometry analysis of multiple samples, to help increase sample throughput to enable relative quantitation of 2 to 18 different samples derived from cells, tissues, or biological fluids
  • Accurate—SureQuant targeted assays and standards provide improved detection enrichment and detection of target proteins for more precise protein quantitation, including low abundant proteins in complex mixtures
  • Broad portfolio—complete offering of kits and reagents to support both targeted and discovery protein quantitation
  • Compatible—optimized for use with Thermo Scientific LC-MS or LC-MS/MS platforms with data analysis fully supported by Proteome Discoverer software

Check out this simple guide and see which protein quantitation technology is right for your research:

Labeling moleculeSample typeMultiplexing sample capabilityTechnology/Reagent
Peptide level (In vitro)Up to 18-plexTandem Mass Tags (TMT)
Peptide level (In vitro)NoneSureQuant Targeted Assays
Protein level (In vivo, Ex vivo)Up to 3-plexStable Isotope Labeling of Amino Acids (SILAC)
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