|Order now||Order now||Order now||Order now|
|SILAC||TMT||Peptides for SRM||SureQuant targeted assays|
|Method of labeling||Metabolic||Amine-, cysteine-, and carbonyl-reactive mass tags||Spike-in standard||Spike-in standard|
|Sample type||Cultured mammalian cells||Cultured mammalian cells;|
|Cultured mammalian cells;|
|Cultured mammalian cells; tissue; biofluids|
|Digestion protocol||In-gel||In solution||In solution||In solution|
|Quantitation mode||MS1||MS2 (6-plex)|
|Multiplex options||2-plex, 3-plex, 4-plex||2-plex, 6-plex, 10-plex, 11-plex||Samples not combined||Samples not combined|
Discovery proteomics maximizes protein identification by spending more time and effort per sample and reducing the number of samples analyzed. In contrast, targeted proteomics strategies limit the number of features that will be monitored, and then optimize the chromatography, instrument tuning and acquisition methods to achieve the highest sensitivity and throughput for larger sample sets.
Rapid, cell-free expression of recombinant proteins containing stable isotope-labeled (i.e., heavy) amino acids can be generated using the 1-Step Heavy Protein IVT Kit. Heavy proteins expressed using this system can be used as mass spectrometry controls for sample preparation loss, digestion efficiency determination or as quantification standards. The Pierce Peptide Retention Time Calibration Mixture contains 15 synthetic heavy peptides mixed at an equimolar ratio that elute across the chromatographic gradient and can be used to assess LC performance as well to predict peptide retention times in targeted assay development.
For Research Use Only. Not for use in diagnostic procedures.