Tandem Mass Tag Systems | Thermo Fisher Scientific

The Thermo Scientific Tandem Mass Tag Reagents are designed to enable identification and quantitation of proteins in different samples using tandem mass spectrometry (MS). The TMT 10plex and 11plex Label Reagents share an identical structure with TMTzero, TMTduplex, and TMTsixplex Reagents but contain different numbers and combinations of 13C and 15N isotopes in the mass reporter.

The new Thermo Scientific TMTpro label reagents now enable up to 16plex sample multiplexing. The TMTpro tag structure is similar to the existing TMT tag structure in that the tags remain isobaric and amine reactive but differ in the longer spacer region and isobutyl proline mass reporter region. After MS/MS fragmentation, each TMTpro tag generates a unique reporter mass (i.e., TMTpro 126-134Da) in the low-mass region of the high-resolution MS/MS spectrum that is used for relative quantitation of protein expression levels. *Note: TMTpro and TMT 6,10 & 11 plex reagents are mutually exclusive and cannot be mixed and matched.

Download the TMTpro 16plex Brochure
Application note - Using TMTpro 16plex reagents with Proteome Discoverer 2.3 software
Apply for a TMT Research Award

Powerful—concurrent MS analysis of multiple samples increases sample throughput and enables relative quantitation of up to 11 (TMT) or 16 (TMTpro) different samples derived from cells, tissues or biological fluids
Robust—increased multiplex capability results in fewer missing quantitative values
Flexible—multiple chemistries allow functional group specific labeling and enrichment, using anti-TMT immobilized resin
Efficient—amine-, cysteine- and carbonyl-reactive chemistries utilize an effective strategy for efficient labeling of target functional group
Compatible—optimized for use with high-resolution MS/MS platforms using SP2 or SPS MS3 quantitation mode with data analysis fully supported by Thermo Scientific Proteome Discoverer Software

View a list of TMT publications in peer-reviewed journals based on topic or disease

Choose the right TMT product for your applications

	Sixplex TMT	10plex TMT	11plex TMT	16plex TMTpro	Sixplex iodoTMT	Sixplex aminoxyTMT
Reactive chemistry	Amine-reactive mass tags	Amine-reactive mass tags	Amine-reactive mass tags	Amine-reactive mass tags	Cysteine-reactive mass tags	Carbonyl-reactive mass tags
Plex	6	10	11	16	6	6
Formats	Labeling reagents & kits	Labeling reagents & kits	Labeling reagents	Labeling reagents	Labeling reagents & kits	Labeling reagents only
Standard packaging	0.8, and 5 mg/vial	0.2, 0.8, and 5 mg/vial	5 mg/vial	0.5 and 5 mg/vial	0.2 mg/vial	0.2 mg/vial
Order now	Reagents (0.8, 5 mg) Kit	Reagents (0.2 mg) Reagents (0.8, 5 mg) Kit	Reagents	Reagents	Reagents Kit	Reagents

TMT reagents data analysis and quantitation

Procedure summary for MS experiments using TMTpro isobaric mass tagging reagents. Protein extracts isolated from cells or tissues are reduced, alkylated, and then digested using the EasyPep™ Mini MS Sample Prep kit or an equivalent method. Samples are then labeled with the TMTpro reagents before sample mixing, fractionation, and cleanup. Labeled samples are analyzed on a high-resolution Orbitrap LC-MS/MS mass spectrometer before data analysis to identify peptides and quantify relative abundance of reporter ions.

Measured induction of S-nitrosylation in phosphoglycerate kinase 1 peptide. BV-2 glioma cells were either untreated or treated with lipopolysaccharide (LPS) or S-nitrosocysteine (SNOC) for 20 hours to induce S-nitrosylation and selectively labeled with Thermo Scientific iodoTMTsixplex Reagents using the S-nitrosylation switch assay. MS spectrum includes phosphoglycerate kinase 1 peptide (GcITIIGGGDTATccAK, inset) showing localization of the iodoTMT-modified cysteine (red). Inserted graph shows an increase in S-nitrosylation of phosphoglycerate kinase 1 peptide in response to S-nitrosylation inducing agents lipopolysaccharide (LPS, 127 & 130) and S-nitrosocysteine (SNOC, 129 &131) determined by relative quantitation of TMT reporter ions in duplicate samples.

Workflow for TMT reagents