The Thermo Scientific Tandem Mass Tag Reagents are designed to enable identification and quantitation of proteins in different samples using tandem mass spectrometry (MS). The TMT 10plex and 11plex Label Reagents share an identical structure with TMTzero, TMTduplex, and TMTsixplex Reagents but contain different numbers and combinations of 13C and 15N isotopes in the mass reporter.

The new Thermo Scientific TMTpro label reagents now enable up to 16plex sample multiplexing. The TMTpro tag structure is similar to the existing TMT tag structure in that the tags remain isobaric and amine reactive but differ in the longer spacer region and isobutyl proline mass reporter region. After MS/MS fragmentation, each TMTpro tag generates a unique reporter mass (i.e., TMTpro 126-134Da) in the low-mass region of the high-resolution MS/MS spectrum that is used for relative quantitation of protein expression levels. *Note: TMTpro and TMT 6,10 & 11 plex reagents are mutually exclusive and cannot be mixed and matched.

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  • Powerful—concurrent MS analysis of multiple samples increases sample throughput and enables relative quantitation of up to 11 (TMT) or 16 (TMTpro) different samples derived from cells, tissues or biological fluids
  • Robust—increased multiplex capability results in fewer missing quantitative values
  • Flexible—multiple chemistries allow functional group specific labeling and enrichment, using anti-TMT immobilized resin
  • Efficient—amine-, cysteine- and carbonyl-reactive chemistries utilize an effective strategy for efficient labeling of target functional group
  • Compatible—optimized for use with high-resolution MS/MS platforms using SP2 or SPS MS3 quantitation mode with data analysis fully supported by Thermo Scientific Proteome Discoverer Software

Choose the right TMT product for your applications

  Amine-reactive 6-plex TMT Sixplex TMT Amine-reactive 10-plex TMT 10plex TMT Amine-reactive 11-plex TMT 11plex TMT TMTpro 16plex Label Reagent Set 16plex TMTpro Cysteine-reactive 6-plex iodoTMT Sixplex iodoTMT Carbonyl-reactive 6-plex aminoxyTMT Sixplex aminoxyTMT
Reactive chemistry Amine-reactive mass tags Amine-reactive mass tags Amine-reactive mass tags Amine-reactive mass tags Cysteine-reactive mass tags Carbonyl-reactive mass tags
Plex 6 10 11 16 6 6
Formats Labeling reagents & kits Labeling reagents & kits Labeling reagents Labeling reagents Labeling reagents & kits Labeling reagents only
Standard packaging 0.8, and 5 mg/vial 0.2, 0.8, and 5 mg/vial 5 mg/vial 0.5 and 5 mg/vial 0.2 mg/vial 0.2 mg/vial
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Featured data

TMTPro workflow
TMT reagents data analysis and quantitation

Procedure summary for MS experiments using TMTpro isobaric mass tagging reagents. Protein extracts isolated from cells or tissues are reduced, alkylated, and then digested using the EasyPep™ Mini MS Sample Prep kit or an equivalent method. Samples are then labeled with the TMTpro reagents before sample mixing, fractionation, and cleanup. Labeled samples are analyzed on a high-resolution Orbitrap LC-MS/MS mass spectrometer before data analysis to identify peptides and quantify relative abundance of reporter ions.

Measured induction of S-nitrosylation in phosphoglycerate kinase 1 peptide.

Measured induction of S-nitrosylation in phosphoglycerate kinase 1 peptide. BV-2 glioma cells were either untreated or treated with lipopolysaccharide (LPS) or S-nitrosocysteine (SNOC) for 20 hours to induce S-nitrosylation and selectively labeled with Thermo Scientific iodoTMTsixplex Reagents using the S-nitrosylation switch assay. MS spectrum includes phosphoglycerate kinase 1 peptide (GcITIIGGGDTATccAK, inset) showing localization of the iodoTMT-modified cysteine (red). Inserted graph shows an increase in S-nitrosylation of phosphoglycerate kinase 1 peptide in response to S-nitrosylation inducing agents lipopolysaccharide (LPS, 127 & 130) and S-nitrosocysteine (SNOC, 129 &131) determined by relative quantitation of TMT reporter ions in duplicate samples.

TMTPro workflow
TMT reagents data analysis and quantitation

Procedure summary for MS experiments using TMTpro isobaric mass tagging reagents. Protein extracts isolated from cells or tissues are reduced, alkylated, and then digested using the EasyPep™ Mini MS Sample Prep kit or an equivalent method. Samples are then labeled with the TMTpro reagents before sample mixing, fractionation, and cleanup. Labeled samples are analyzed on a high-resolution Orbitrap LC-MS/MS mass spectrometer before data analysis to identify peptides and quantify relative abundance of reporter ions.

Measured induction of S-nitrosylation in phosphoglycerate kinase 1 peptide.

Measured induction of S-nitrosylation in phosphoglycerate kinase 1 peptide. BV-2 glioma cells were either untreated or treated with lipopolysaccharide (LPS) or S-nitrosocysteine (SNOC) for 20 hours to induce S-nitrosylation and selectively labeled with Thermo Scientific iodoTMTsixplex Reagents using the S-nitrosylation switch assay. MS spectrum includes phosphoglycerate kinase 1 peptide (GcITIIGGGDTATccAK, inset) showing localization of the iodoTMT-modified cysteine (red). Inserted graph shows an increase in S-nitrosylation of phosphoglycerate kinase 1 peptide in response to S-nitrosylation inducing agents lipopolysaccharide (LPS, 127 & 130) and S-nitrosocysteine (SNOC, 129 &131) determined by relative quantitation of TMT reporter ions in duplicate samples.

Featured Video

TMT10plex reagents in action

Learn how to prepare and label peptide samples with tandem mass tags for quantitative proteomics analysis.


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