Automated Magnetic Separations for Proteomics
Accelerate proteomics workflow throughput (HTS) using magnetic separations
Speed and sample purity are major concerns for proteomics researchers, and strategies to achieve higher throughput for sample-handling and data-processing are desired. Magnetic particles comprise a convenient solid support for a variety of assays and procedures based on affinity purification. Paramagnetic beads are especially well suited for automated procedures because instrumentation exists to easily mix, incubate and separate the particles in 96-well plates without columns or centrifugation. Such ease of manipulation enables rapid processing of many samples and seemless integration and compatibility with entire workflows.
Automation-validated Pierce Magnetic Beads
Thermo Scientific™ Pierce™ Magnetic Beads are high-performance, iron oxide, superparamagnetic particles that are derivatized with different ligands for use in a variety of affinity purification methods. The beads are designed and validate for use with automated magnetic particle processors, including high-throughput magnetic platforms. The beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand.
Pierce Magnetic Beads and Kits
|Magnetic Bead||Affinity Target||Magnetic Bead Kits|
|Anti-HA||HA-tagged fusion proteins||HA-Tag Magnetic IP/Co-IP Kit|
|Anti-c-Myc||Myc-tagged fusion proteins||Myc-Tag Magnetic IP/Co-IP Kit|
|Ni-NTA||His-tagged fusion proteins|
|Glutathione||Glutathione S-transferase (GST) fusion proteins|
|NHS ester||Covalent immobilization of primary amine molecules
(proteins, peptides, etc.) for affinity purification
|Direct Magnetic IP Kit|
|Protein A/G||Antibodies (IgG containing Fc)||Classic Magnetic IP Kit
Crosslink Magnetic IP Kit
|Protein A||Antibodies (IgG containing Fc)|
|Protein G||Antibodies (IgG containing Fc)|
|Protein L||Antibodies (Ig containing kappa light chains)|
|Streptavidin||Biotin or desthiobiotin|
|Titanium Dioxide||Phosphopeptides containing phospho Ser, Tyr or Thr||Phosphopeptide Enrichment Kit|
KingFisher Magnetic Particle Processors
Prepare samples efficiently from a variety of materials with Thermo Scientific™ KingFisher™ Purification Systems, which offer highly versatile, automated magnetic-particle processing for DNA/RNA, protein or cell purification from virtually any source. Using revolutionary magnetic particle separation technology, these systems provide excellent reproduciblity and quality. Thermo Scientific BindIt™ Software provides integrated or remote control of KingFisher Instruments. Ready-to-use BindIt Software protocols (.bdz files) are available for variety of applications with Pierce Magnetic Beads.
Broad Instrument Compatibility
In addition to being validated with KingFisher™ Instruments, Pierce Magnetic Beads are compatible with other instrumentation designed for magnetic particle processing (see table below). The beads are also compatible with manual separations using an appropriate magnetic stand.
|KingFisher™ Flex and Duo||Thermo Fisher Scientific|
|Biomek™ FX||Beckman Instruments|
|Invitrogen™ iPrep™||Life Technologies, now
Thermo Fisher Scientific
|DNATrak™, Evolution™, MultiPROBE™ II||Perkin Elmer|
|Biorobot™, QIAsymphony™, QIAcube™||Qiagen|
|Magna Pure™ LC||Roche|
|Genesis FE500™, Freedom EVO™||Tecan|
|Sciclone™ ALH 3000||Caliper Life Sciences|
Complete Workflow Solutions
Together with instruments for magnetic particle processing and mass spectrometry, Pierce Magnetic Beads and cell lysis reagents provide a complete workflow solution for proteomics research. Products from most magnetic bead procedures are also compatible with electrophoresis and Western blot workflows.
For Research Use Only. Not for use in diagnostic procedures.