Workflow for Protein Mass Spectrometry
Mass spectrometry ("mass spec" or MS) is a powerful analytical tool for proteomics research and drug discovery. MS enables identification and quantification of known and unknown compounds by revealing their structural and chemical properties. Proper sample preparation for MS-based analysis is a critical step in the proteomics workflow because the quality and reproducibility of sample extraction and preparation for downstream analysis significantly impacts the separation and identification capabilities of mass spectrometers.
Because the proteome is so complex, there is no one standard method for preparing protein samples for MS analysis. Protocols differ depending on the type of sample, experiment and method of analysis. For example, preparing samples from a biological fluid involves a different set of procedures than preparing samples from cell culture or tissue. Many factors must be considered, including the source, type, physical properties, abundance, complexity, matrix effects and cellular location of the target proteins.
Our scientists recognize the need for integrated proteomics solutions and continually strive to develop products and technologies that are compatible and supportive of MS analysis. The following workflow highlights options for preparing samples from a variety of starting materials for successful mass spec analysis.
Mass spectrometry workflow
Reagents for MS Instrument Calibration, QC, and Analysis
Peptide Clean Up
Peptide Enrichment and/or Fractionation
Peptide or Glycan Labeling
Protein Clean Up and Quantitation
Protein Labeling and/or Enrichment
Abundant Protein Depletion
serum, plasma, & biofluids
For Research Use Only. Not for use in diagnostic procedures.