Cell Lysis (Total Protein Extraction)
Extract total protein from mammalian tissue or cell samples using mild but efficient lysis reagents. These reagents have been optimized and validated with specific tissue types, as well as in primary and cultured mammalian cells. Protein extracts are compatible with most protein assays and typical downstream applications. These reagents offer higher protein yield than other commercially available or homemade formulations.
These convenient single formulation reagents typically allow samples to be processed in 5–10 minutes. When lysing primary and cultured cells, mechanical disruption is not required.
Choose a total protein extraction reagent for your experiments
|T-PER Tissue Protein Extraction Reagent||N-PER Neuronal Protein Extraction Reagent||M-PER Mammalian Protein Extraction Reagent||RIPA Lysis Buffer||IP Lysis Buffer|
|Compatible sample types||Heart, liver, kidney, lung, spleen||Brain tissue and primary neurons||Cultured mammalian and primary cells||Cultured mammalian cells||Cultured mammalian cells|
|Sample processing time||10 min||5–10 min||5 min||10 min||10 min|
|Mechanical disruption required?||Yes||Yes, for tissue samples||No||No||No|
|Sample amount||25 g fresh tissue per 500 mL||10 g tissue or 200 x 10 cm dishes of primary cultured neurons per 100 mL||25 g of wet cells (10 million cells per 1 mL of reagent)||5 million cells per 1 mL of reagent||5 million cells per 1 mL of reagent|
|Protein assay compatibility||BCA, Coomassie Plus, Pierce 660, Detergent Compatible Bradford||BCA, Pierce 660, Detergent Compatible Bradford||BCA, Coomassie Plus, Pierce 660, Detergent Compatible Bradford||BCA, Detergent Compatible Bradford (dilute 1:3)||BCA, Pierce Detergent Compatible Bradford|
|Downstream compatibility||IP, western blots, ELISA, EMSA, purification, kinase assays, activity assays & amine reactive labeling||IP, pulldowns, western blots, ELISA, enzyme assays & amine reactive labeling||IP, western blots, ELISA, EMSA, reporter assays, purification, activity assays & amine reactive labeling||Western blots, ELISA & purification||Western blots, ELISA,
IP & reporter assays
|Protease or phosphatase inhibitors recommended?||Yes, both||Yes, both||Yes, both||Yes, both||Yes, both|
|Order now||Order now||Order now||Order now||Order now|
Additional cell lysis products
Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.
Extract soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture with this gentle and effective lysis reagent.
For Research Use Only. Not for use in diagnostic procedures.