Membrane Protein Extraction and Isolation showcase

Our membrane protein isolation kits are optimized for membrane protein isolation and enrichment. The isolation of high-quality protein is essential for successful completion of downstream applications. Reagents in our kits enable high protein yield, preserve protein function, and have been optimized for minimum contamination. These isolated membrane proteins are also compatible with multiple protein assays and other typical downstream applications.

Cell Fractionation KitsCell Lysis Buffers

Membrane protein isolation kits

 GPCR Extraction and Stabilization ReagentCell Surface Protein Biotinylation and Isolation KitMem-PER Plus Membrane Protein Extraction Kit
Membrane protein enrichmentG Protein-Coupled Receptors (GPCR)Plasma membrane proteinsIntegral and membrane associated proteins
Protein functionalityMaintains structural integrity as measured by immunoprecipitation and receptor ligand binding assaysEnrichment compatible with western blotting and MS applicationsEnrichment compatible with western blotting
Compatible sample typesTissues and cultured mammalian cellsTissues and cultured mammalian cellsCultured mammalian cells
Cytosolic contaminationWhole cell lysateLess than 5% contamination of cytosolic proteinsLess than 20% contamination of cytosolic proteins
Sample processing time1–2 hours1 hour2 hours
Mechanical disruption required?Yes, for tissueYes, for tissueNo
Amount of sample processed100 samples with 107 cells or 100 samples with 50–100 mg tissue50 samples with 5 million cells or 25 samples of 20–40 mg tissue per kit8 experiments, with two confluent 15 cm dishes or four T75 flasks
Protein assay compatibilityBCA Protein AssaysBCA Protein Assays, detergent-compatible BradfordLysis buffer is compatible with BCA and Rapid Gold BCA; elution buffer is not compatible.
Downstream compatibilityWestern blot, IP, protein purification, radio-ligand binding assaysIP, western blot, ELISA, amine reactive labelingWestern blot, ELISA, mass spectrometry analysis
Protease or phosphatase inhibitors recommended?Yes, bothYes, bothYes, both
Cat. No.A43436A4439089842

Reagent-based membrane protein extraction and membrane protein isolation

Reagent-based lysis has replaced traditional physical lysis as the method of choice for membrane disruption and extraction of membrane proteins. Reagent-based lysis methods do not require expensive, cumbersome equipment and protocols that are difficult to implement. The Thermo Scientific membrane protein extraction and membrane protein isolation reagents consist of optimized concentrations of detergents, buffers, salts, and reducing agents developed for particular species and types of cells. Reagents also have the added benefits of both lysing and solubilizing effects. Our membrane protein isolation and membrane protein extraction kits are optimized specifically for your needs to isolate high-quality protein samples essential to completing successful downstream applications.

Features:

  • Extraction and isolation—produces minimal cross-contamination (typically less than 10%) of cytosolic protein into the membrane protein fraction
  • Cells or tissues—effective for extraction from cultured mammalian cells and mammalian tissues
  • Downstream compatibility—analyze membrane protein extracts by SDS-PAGE, western blotting, immunoprecipitation, and protein assays
  • Fast and simple—isolation of membrane proteins in approximately one hour
  • No special equipment required—only a benchtop microcentrifuge, tubes, homogenizers, and pipettors are needed

Strategies for isolation of plasma membrane proteins

This webinar will focus on robust and optimized techniques for extraction, isolation and enrichment of cell surface proteins, including stable and functional G protein-coupled receptors (GPCRs).

Membrane protein isolation kits

 GPCR Extraction and Stabilization ReagentCell Surface Protein Biotinylation and Isolation KitMem-PER Plus Membrane Protein Extraction Kit
Membrane protein enrichmentG Protein-Coupled Receptors (GPCR)Plasma membrane proteinsIntegral and membrane associated proteins
Protein functionalityMaintains structural integrity as measured by immunoprecipitation and receptor ligand binding assaysEnrichment compatible with western blotting and MS applicationsEnrichment compatible with western blotting
Compatible sample typesTissues and cultured mammalian cellsTissues and cultured mammalian cellsCultured mammalian cells
Cytosolic contaminationWhole cell lysateLess than 5% contamination of cytosolic proteinsLess than 20% contamination of cytosolic proteins
Sample processing time1–2 hours1 hour2 hours
Mechanical disruption required?Yes, for tissueYes, for tissueNo
Amount of sample processed100 samples with 107 cells or 100 samples with 50–100 mg tissue50 samples with 5 million cells or 25 samples of 20–40 mg tissue per kit8 experiments, with two confluent 15 cm dishes or four T75 flasks
Protein assay compatibilityBCA Protein AssaysBCA Protein Assays, detergent-compatible BradfordLysis buffer is compatible with BCA and Rapid Gold BCA; elution buffer is not compatible.
Downstream compatibilityWestern blot, IP, protein purification, radio-ligand binding assaysIP, western blot, ELISA, amine reactive labelingWestern blot, ELISA, mass spectrometry analysis
Protease or phosphatase inhibitors recommended?Yes, bothYes, bothYes, both
Cat. No.A43436A4439089842

Reagent-based membrane protein extraction and membrane protein isolation

Reagent-based lysis has replaced traditional physical lysis as the method of choice for membrane disruption and extraction of membrane proteins. Reagent-based lysis methods do not require expensive, cumbersome equipment and protocols that are difficult to implement. The Thermo Scientific membrane protein extraction and membrane protein isolation reagents consist of optimized concentrations of detergents, buffers, salts, and reducing agents developed for particular species and types of cells. Reagents also have the added benefits of both lysing and solubilizing effects. Our membrane protein isolation and membrane protein extraction kits are optimized specifically for your needs to isolate high-quality protein samples essential to completing successful downstream applications.

Features:

  • Extraction and isolation—produces minimal cross-contamination (typically less than 10%) of cytosolic protein into the membrane protein fraction
  • Cells or tissues—effective for extraction from cultured mammalian cells and mammalian tissues
  • Downstream compatibility—analyze membrane protein extracts by SDS-PAGE, western blotting, immunoprecipitation, and protein assays
  • Fast and simple—isolation of membrane proteins in approximately one hour
  • No special equipment required—only a benchtop microcentrifuge, tubes, homogenizers, and pipettors are needed

Strategies for isolation of plasma membrane proteins

This webinar will focus on robust and optimized techniques for extraction, isolation and enrichment of cell surface proteins, including stable and functional G protein-coupled receptors (GPCRs).

Related products and downstream applications