Our membrane protein isolation kits are optimized for membrane protein isolation and enrichment. The isolation of high quality protein is essential for successful completion of downstream applications. Reagents in our kits enable high protein yield, preserve protein function, and have been optimized for minimum contamination. These isolated membrane proteins are also compatible with multiple protein assays and other typical downstream applications.
Strategies for isolation of plasma membrane proteins
This webinar will focus on robust and optimized techniques for extraction, isolation and enrichment of cell surface proteins, including stable and functional G protein-coupled receptors (GPCRs).
Select a membrane protein extraction kit or membrane protein isolation kit for your experiment
|GPCR Extraction and Stabilization Reagent||Cell Surface Protein Biotinylation and Isolation Kit||Mem-PER Plus Membrane Protein Extraction Kit|
|Membrane protein enrichment||Whole cell lysate optimized for solubilization of GPCRs||Enriches plasma membrane proteins||Enriches proteins from membrane bound organelles|
|Protein functionality||Maintains structural integrity as measured by immunoprecipitation and receptor ligand binding assays||Enrichment compatible with western blotting and MS applications||Enrichment compatible with western blotting|
|Cytosolic contamination||Whole cell lysate||Less than 5% contamination of cytosolic proteins||Less than 20% contamination of cytosolic proteins|
|Order now||Order Now||Order now|
Reagent-based membrane protein extraction and membrane protein isolation
In recent years, reagent-based lysis has replaced traditional physical lysis as the method of choice for membrane disruption and extraction of membrane proteins. Reagent-based lysis methods do not require expensive, cumbersome equipment and protocols that are difficult to implement. The new generation of membrane protein extraction and membrane protein isolation reagents consist of optimized concentrations of detergents, buffers, salts and reducing agents developed for particular species and types of cells. Reagents also have the added benefits of both lysing and solubilizing effects.
Cell or tissue disruption methods. Many cell lysis methods have been developed to obtain the best possible yield and purity for different species of organisms, sample types (cells or tissue), and target molecule or subcellular structure. Cell lysis methods can be divided into two main categories, reagent-based and physical disruption.
Our membrane protein isolation and membrane protein extraction kits are optimized specifically for your needs to isolate high quality protein samples essential to completing successful downstream applications.
Isolation of high quality protein: Quality or physical form of the isolated protein is an important consideration when extracting proteins for certain downstream applications.
For Research Use Only. Not for use in diagnostic procedures.