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Dynabeads® Magnetic Beads can be used to isolate a number of different organelles and organelle-specific proteins. This magnetic separation technology enables the specific isolation of true fractions in small volumes, and can replace time-consuming and labor-intensive density gradient centrifugations.

Advantages include:

  • Rapid, high-purity isolations from crude fractions or fractions of common densities
  • Morphologically intact target organelles can be fixed for EM studies or solubilized directly on the bead surface
  • Immunomagnetically isolated organelles can be assayed by conventional techniques
  • Easy protocol yields high-purity functional organelles

As more and more organelle-specific markers are identified, the corresponding immunomagnetic isolation of subgroups of organelles can be performed. Examples of organelles or organelle fractions that have been isolated with Dynabeads® include exosomesmitochondriaperoxisomeskidney glomeruli, and endondocytic and secretory compartments.

View selected Dynabeads® references

Compare Dynabeads® organelle isolation methods

1. Select your application Application specification 2. Click on the Solution Advantage
Isolation of fragile organelles or organelle fractions in general Using a mouse primary antibody Dynabeads® M-280 Sheep anti-Mouse IgG Direct or indirect isolation of organelles made more efficient with two layers of Abs.
Using a rabbit primary antibody Dynabeads® M-280 Sheep anti-Rabbit IgG Direct or indirect isolation of organelles made more efficient with two layers of Abs.
Antibodies made in any other species Dynabeads® Protein G 
Dynabeads® Protein A
Efficient direct or indirect isolation of organelles.
Isolation of large organelle fractions or isolation from a viscous sample Using antibodies made in a specific species Dynabeads® M-450 with a secondary antibody against the corresponding primary Ab species More efficient pull to the magnet in viscous samples
Isolation of organelles using a ligand that is not an antibody When high yield is important Dynabeads® M-280 Tosylactivated Easy coupling of a molecule onto these hydrophobic beads for efficient organelle isolation
Isolation of organelles using a ligand that is not an antibody When high purity is important Dynabeads® M-270 Epoxy Coupling of a molecule onto these hydrophillic beads for very high purity organelle isolation