Organelle Isolation Using Magnetic Beads
Dynabeads® Magnetic Beads can be used to isolate a number of different organelles and organelle-specific proteins. This magnetic separation technology enables the specific isolation of true fractions in small volumes, and can replace time-consuming and labor-intensive density gradient centrifugations.
- Rapid, high-purity isolations from crude fractions or fractions of common densities
- Morphologically intact target organelles can be fixed for EM studies or solubilized directly on the bead surface
- Immunomagnetically isolated organelles can be assayed by conventional techniques
- Easy protocol yields high-purity functional organelles
As more and more organelle-specific markers are identified, the corresponding immunomagnetic isolation of subgroups of organelles can be performed. Examples of organelles or organelle fractions that have been isolated with Dynabeads® include exosomes, mitochondria, peroxisomes, kidney glomeruli, and endondocytic and secretory compartments.
Compare Dynabeads® organelle isolation methods
|1. Select your application||Application specification||2. Click on the Solution||Advantage|
|Isolation of fragile organelles or organelle fractions in general||Using a mouse primary antibody||Dynabeads® M-280 Sheep anti-Mouse IgG||Direct or indirect isolation of organelles made more efficient with two layers of Abs.|
|Using a rabbit primary antibody||Dynabeads® M-280 Sheep anti-Rabbit IgG||Direct or indirect isolation of organelles made more efficient with two layers of Abs.|
|Antibodies made in any other species||Dynabeads® Protein G
Dynabeads® Protein A
|Efficient direct or indirect isolation of organelles.|
|Isolation of large organelle fractions or isolation from a viscous sample||Using antibodies made in a specific species||Dynabeads® M-450 with a secondary antibody against the corresponding primary Ab species||More efficient pull to the magnet in viscous samples|
|Isolation of organelles using a ligand that is not an antibody||When high yield is important||Dynabeads® M-280 Tosylactivated||Easy coupling of a molecule onto these hydrophobic beads for efficient organelle isolation|
|Isolation of organelles using a ligand that is not an antibody||When high purity is important||Dynabeads® M-270 Epoxy||Coupling of a molecule onto these hydrophillic beads for very high purity organelle isolation|
For Research Use Only. Not for use in diagnostic procedures.