Lysis-Extraction-Art-230

Cell lysis and organelle analysis

These kits are optimized for stepwise separation, enrichment and extraction of proteins from different cell fractions, including cytoplasmic, membrane, nuclear, chromatin-bound, and cytoskeletal proteins in 1–3 hrs. Most fractions are compatible with protein assays and typical downstream applications.

Select the right subcellular fractionation kit for your mammalian tissues or cells

  GPCR Extraction & Stabilization Reagent Mem-PER Plus Membrane Protein Extraction Kit Cell Surface Protein Isolation Kit
Compatible sample types Tissues and cultured mammalian cells Tissues and cultured mammalian cells Cultured mammalian cells
Fractions isolated GPCR enriched fraction Integral and membrane associated Surface membrane proteins
Sample processing time 1-2 hrs 1 hr

2 hrs

Mechanical disruption required? Yes, for tissue Yes, for tissue No
Amount of sample processed 100 samples with 107 cells or 100 samples with 50-100mg tissue 50 samples with 5 million cells or 25 samples of 20 to 40 mg tissue per kit 8 experiments, with two confluent 15cm dishes or four T75 flasks
Protein assay compatibility Rapid Gold BCA, BCA, Micro BCA, Pierce 660 BCA, Pierce 660, detergent compatible Bradford Lysis buffer compatible with BCA and Rapid Gold BCA.  Elution buffer not compatible
Downstream compatibility Western blot, IP, protein purification, radio-ligand binding assays IP, western blot, ELISA, amine reactive labeling Western blot, ELISA, mass spectrometry analysis
Protease or phosphatase inhibitors recommended? Yes, both Yes, both Yes, both
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  NE-PER Nuclear and Cytoplasmic Extraction Reagents Subcellular protein fractionation kits (cells or tissues) Syn-PER Synaptic Protein Extraction Reagent
Compatible sample types Tissues and cultured mammalian cells Tissues or cultured mammalian cells Brain tissue and primary neurons
Fractions isolated Nucleus, cytoplasm Nucleus, cytoplasm, membrane, cytoskeletal, chromatin bound Synaptic Proteins
Sample processing time 2 hrs 2–3 hrs <1 hr
Mechanical disruption required? Yes, for tissue Yes, for tissue Yes, for tissue
Amount of sample processed 50 samples with 2 million cells (20 µL packed) 25 extractions of 200 mg tissue or 50 samples with 2 million cells (20 µL packed) 10 g tissue or 500 x 35 mm dishes of primary cultured neurons
Protein assay compatibility BCA (CER dilute 1:4), Coomassie™ Plus (CER dilute 1:4), Pierce 660, detergent compatible Bradford BCA (all fractions), Pierce 660 (except PEB); NEB also compatible with Coomassie™ Plus and detergent compatible Bradford BCA
Downstream compatibility Both: western blot, ELISA, EMSA, reporter assays, enzyme assays, amine reactive labeling
CER only: RNA EMSA, kinase assays, and RT-PCR
IP, western blot, ELISA, EMSA, reporter assays, enzyme assays, amine reactive labeling Neurotransmitter release assays, enzyme assays immunoassays, chromatography,
electrophoresis
Protease or phosphatase inhibitors recommended? Yes, both Protease inhibitors included in kit Yes, both
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  GPCR Extraction & Stabilization Reagent Mem-PER Plus Membrane Protein Extraction Kit Cell Surface Protein Isolation Kit
Compatible sample types Tissues and cultured mammalian cells Tissues and cultured mammalian cells Cultured mammalian cells
Fractions isolated GPCR enriched fraction Integral and membrane associated Surface membrane proteins
Sample processing time 1-2 hrs 1 hr

2 hrs

Mechanical disruption required? Yes, for tissue Yes, for tissue No
Amount of sample processed 100 samples with 107 cells or 100 samples with 50-100mg tissue 50 samples with 5 million cells or 25 samples of 20 to 40 mg tissue per kit 8 experiments, with two confluent 15cm dishes or four T75 flasks
Protein assay compatibility Rapid Gold BCA, BCA, Micro BCA, Pierce 660 BCA, Pierce 660, detergent compatible Bradford Lysis buffer compatible with BCA and Rapid Gold BCA.  Elution buffer not compatible
Downstream compatibility Western blot, IP, protein purification, radio-ligand binding assays IP, western blot, ELISA, amine reactive labeling Western blot, ELISA, mass spectrometry analysis
Protease or phosphatase inhibitors recommended? Yes, both Yes, both Yes, both
  Order now Order now Order now
  NE-PER Nuclear and Cytoplasmic Extraction Reagents Subcellular protein fractionation kits (cells or tissues) Syn-PER Synaptic Protein Extraction Reagent
Compatible sample types Tissues and cultured mammalian cells Tissues or cultured mammalian cells Brain tissue and primary neurons
Fractions isolated Nucleus, cytoplasm Nucleus, cytoplasm, membrane, cytoskeletal, chromatin bound Synaptic Proteins
Sample processing time 2 hrs 2–3 hrs <1 hr
Mechanical disruption required? Yes, for tissue Yes, for tissue Yes, for tissue
Amount of sample processed 50 samples with 2 million cells (20 µL packed) 25 extractions of 200 mg tissue or 50 samples with 2 million cells (20 µL packed) 10 g tissue or 500 x 35 mm dishes of primary cultured neurons
Protein assay compatibility BCA (CER dilute 1:4), Coomassie™ Plus (CER dilute 1:4), Pierce 660, detergent compatible Bradford BCA (all fractions), Pierce 660 (except PEB); NEB also compatible with Coomassie™ Plus and detergent compatible Bradford BCA
Downstream compatibility Both: western blot, ELISA, EMSA, reporter assays, enzyme assays, amine reactive labeling
CER only: RNA EMSA, kinase assays, and RT-PCR
IP, western blot, ELISA, EMSA, reporter assays, enzyme assays, amine reactive labeling Neurotransmitter release assays, enzyme assays immunoassays, chromatography,
electrophoresis
Protease or phosphatase inhibitors recommended? Yes, both Protease inhibitors included in kit Yes, both
  Order now

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Subcellular fractionation overview