Protein Dialysis, Desalting, and Concentration
After cell lysis or isolation, lysates often contain contaminants that are not compatible with downstream steps. We offer a variety of specialty devices and resins that simply and efficiently desalt, exchange buffer, and remove detergents from samples. In addition, if the protein concentration is too dilute for further processing or analysis, the sample can be concentrated quickly using centrifugal concentrators.
Choose the right protein clean-up product for your experiments
|Dialysis devices, cassettes, 96-well plates and tubing||Desalting spin columns, 96-well spin plates and cartridges||Concentrators||Detergent removal spin columns and 96-well spin plates|
|Sample volume processing range||10 μL–250 mL||2 μL–4 mL||100 μL–100 mL||25 μL–1 mL|
|Sample processing time (volume dependent)||2–24 hrs||5–10 min||5–30 min*||15–20 min|
|Key applications||Buffer exchange, desalting, virus purification||Desalting, buffer exchange, removal of unincorporated label||Sample concentration, desalting, buffer exchange||Removal of anionic, nonionic and zwitterionic detergents|
|Recommended sample type||Purified protein||Lysate or purified protein||Lysate or purified protein||Purified protein or peptide mixture|
|Compatible with viscous samples?||Yes||No||No||No|
|Sample diluted during processing?||Possible||No||No||No|
|Gamma-irradiated options available?||Yes||No||No||No|
* for 10K MWCO (device dependent—times may vary for other MWCO’s)
Featured dialysis, desalting, and concentration categories
Slide-A-Lyzer™ devices, cassettes, and flasks in a variety of formats and MWCOs, Pierce™ 96-well microdialysis plates, & SnakeSkin™ Dialysis Tubing
Quickly remove salts and small molecules from protein solutions using Thermo Scientific™ Zeba™ Spin Columns, plates, and cartridges
Thermo Scientific™ Pierce™ Concentrators are available in four distinct molecular weight cutoffs (MWCO) for volumes ranging between 100 μL – 100 mL
Detergent removal resins in two formulations—optimized for high- or low-peptide concentrations—available in spin columns and 96-well spin filter plates
The Thermo Scientific™ Pierce™ RED system with easy-to-use inserts or preloaded plates for automation-compatible plasma protein binding assays
We offer reliable products to help you effectively isolate peptides, proteins, and protein complexes directly from your crude sample
Available in multiple formats, Thermo Scientific™ Pierce™ High-Capacity Endotoxin Removal Resin selectively binds and removes endotoxins from protein, peptide, and antibody samples using a modified ε-poly-L-lysine [poly(ε-lysine)] affinity ligand. Endotoxin levels in biological samples are reduced by ≥99% in as fast as 1 hour using our spin column format, with ≥85%. protein recovery.
For Research Use Only. Not for use in diagnostic procedures.