Protein Dialysis, Desalting, and Concentration

After cell lysis or isolation, lysates often contain contaminants that are not compatible with downstream steps. We offer a variety of specialty devices and resins that simply and efficiently desalt, exchange buffer, and remove detergents from samples. In addition, if the protein concentration is too dilute for further processing or analysis, the sample can be concentrated quickly using centrifugal concentrators.

To address the need for rapid removal of small molecules from protein sample preparations, we have expanded our offering with the Pierce Dye and Biotin Removal Spin Columns and 96-Well Filter Plates. These products facilitate removal of non‑conjugated fluorescent dyes, biotin, unused reducing agents, crosslinkers, and other reaction byproducts with excellent protein recovery.

Choose the right protein clean-up product for your experiments

  Dyes and biotin removal columns Dialysis cassettes and devices Desalting spin columns Detergent removal spin Concentrators
Sample volume processing range 50 μL–4 mL 10 μL–250 mL 2 μL–4 mL 25 μL–1 mL 100 μL–100 mL
Sample processing time (volume dependent) 5–15 min 2–24 hrs 5–10 min 15–20 min 5–30 min*
Key applications Removal of unreacted/unincorporated fluorescent dyes, biotins, crosslinkers, and reducing agents during post-reaction cleanup Buffer exchange, desalting, virus purification Desalting, buffer exchange, removal of unincorporated label Removal of anionic, nonionic and zwitterionic detergents Sample concentration, desalting, buffer exchange
Recommended sample type Purified protein Purified protein Lysate or purified protein Purified protein or peptide mixture Lysate or purified protein
Compatible with viscous samples? No Yes No No No
Sample diluted during processing? No Possible No No No
Gamma-irradiated options available? No Yes No No No

* for 10K MWCO (device dependent—times may vary for other MWCO’s)

Pierce Dye and Biotin Removal columns enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from samples containing proteins of interest that are larger than 7 kDa.

Thermo Scientific Pierce Concentrators are available in four distinct molecular weight cutoffs (MWCO) for volumes ranging between 100 μL – 100 mL.

Slide-A-Lyzer devices, cassettes, and flasks in a variety of formats and MWCOs, Pierce 96-well microdialysis plates, & SnakeSkin Dialysis Tubing.

Detergent removal resins in two formulations—optimized for high- or low-peptide concentrations—available in spin columns and 96-well spin filter plates.

Quickly remove salts and small molecules from protein solutions using Thermo Scientific Zeba Spin Columns, plates, and cartridges.

The Thermo Scientific Pierce RED system with easy-to-use inserts or preloaded plates for automation-compatible plasma protein binding assays.

Related categories

Mini- and microcentrifuges to help accelerate your protein preparation protocols with RCFs up to 21,000 x g in compact, safe, easy-to-use instruments.

Designed for solid performance and consistent results, our benchtop centrifuges deliver efficient sample processing for your protein purification & isolation applications.

Endotoxin removal

Available in multiple formats, Thermo Scientific Pierce High-Capacity Endotoxin Removal Resin selectively binds and removes endotoxins from protein, peptide, and antibody samples using a modified ε-poly-L-lysine [poly(ε-lysine)] affinity ligand. Endotoxin levels in biological samples are reduced by ≥99% in as fast as 1 hour using our spin column format, with ≥85% protein recovery.