Thermo Scientific™ Slide-A-Lyzer™ Dialysis Cassettes help facilitate the rapid and effective dialysis of sample volumes ranging from 100 μL to 30 mL. The cassette design helps maximize surface area to sample volume ratio and enables excellent sample recoveries.
In comparison to standard flat tubing, these innovative cassettes do not require knots or clips that may lead to leaking and sample loss, helping to obtain more complete sample recovery. They are available in 4 volume capacities and in molecular weight cutoffs (MWCOs) of 2K, 3.5K, 7K, 10K, and 20K.
Choose your Slide-A-Lyzer cassette by volume & molecular weight cutoff (MWCO)
| Volume range | ||||
|---|---|---|---|---|
| Molecular weight cut off | 0.1–0.5 mL | 0.5–3 mL | 3–12 mL | 12–30 mL |
| 2K | Catalog number 66205 | Catalog number 66203 | Catalog number 66212 | Catalog number 66230 |
| 3.5K | Catalog number 66333 66335 (kit) | Catalog number 66330 66332 (kit) | Catalog number 66110 66107 (kit) | Catalog number 66130 |
| 7K | Catalog number 66373 | Catalog number 66370 66372 (kit) | Catalog number 66710 66707 (kit) | Not available |
| 10K | Catalog numbers 66383, 66384 66385 (kit) | Catalog numbers 66380, 66381 66382 (kit) | Catalog numbers 66810, 66811 66807 (kit) | Catalog number 66830 |
| 10K (gamma irradiated) | Catalog number 66454 | Catalog number 66455 | Catalog number 66453 | Catalog number 66456 |
| 20K | Catalog number 66005 | Catalog number 66003 | Catalog number 66012 | Catalog number 66030 |
Learn more about Slide-A-Lyzer Cassettes
Table 1. Quantitative sample recovery.
Three sample volume batches of water (0.5 mL, 1.7 mL and 3.0 mL) were loaded and recovered per the respective manufacturer’s instructions in a Slide-A-Lyzer Dialysis Cassette or conventional dialysis tubing to determine the volumes of recovery. Water volume recovered was determined gravimetrically.
| Sample volume loaded | Thermo Scientific Slide-A-Lyzer Dialysis Cassette % volume recovery | Traditional dialysis tubing % volume recovery |
|---|---|---|
| 3.0 mL | 99.47 | 92.32 |
| 1.7 mL | 99.30 | 93.12 |
| 0.5 mL | 98.76 | 87.51 |
Thermo Scientific Slide-A-Lyzer Cassette procedure summary
1. Insert syringe needle through the gasket via one of the corner ports. Inject the sample, withdraw the excess air and remove the syringe.
2. Attach a float buoy and dialyze. (Buoys also serve as convenient bench-top stands for the cassettes.)
3. Insert empty syringe needle at a second corner port. Inject air to expand the cassette chamber, then withdraw the dialyzed sample.
Thermo Scientific Slide-A-Lyzer Dialysis Cassette Video
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