Photo showing the assorted volumes of the Slide-A-Lyzer G3 Dialysis Cassette from volumes 3 mL to 125 mL

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes and Flasks offer an easy-to-use and convenient alternative to dialysis tubing for samples of a variety of volumes (from 1–250 mL) and molecular weight cutoffs (MWCOs). With rigid, free-standing frames, large-surface-area regenerated cellulose membranes, and a locking cap, these devices allow for easy sample loading/retrieval while offering enhanced efficiency and sample security for your dialysis experiments.

Slide-A-Lyzer Dialysis Cassettes

The newest generation of Slide-A-Lyzer, the G3 features a more compact shape, improved self-flotation, significantly less plastic waste and a new, secure cap design with wider opening that allows for easier loading and retrieval with serological pipette. They are available in five volume capacities and in 2K, 3.5K, 10K, and 20K MWCOs.

Choose the right Slide-A-Lyzer G3 dialysis cassette for your experiments

 
 Volume range
MWCO1–3 mL5–15 mL10–30 mL25–70 mL50–125 mL
2KSlide-A-Lyzer G3
3 mL/2K
A52961
Slide-A-Lyzer G3
15 mL/2K
A52962
Slide-A-Lyzer G3
30 mL/2K
A52963
Slide-A-Lyzer G3
70 mL/2K
A52964
Slide-A-Lyzer G3
125 mL/2K
A52965
3.5KSlide-A-Lyzer G3
3 mL/3.5K
A52966
Slide-A-Lyzer G3
15 mL/3.5K
A52967
Slide-A-Lyzer G3
30 mL/3.5K
A52968
Slide-A-Lyzer G3
70 mL/3.5K
A52969
Slide-A-Lyzer G3
125 mL/3.5K
A52970
10KSlide-A-Lyzer G3
3 mL/10K
A52971
Slide-A-Lyzer G3
15 mL/10K
A52972
Slide-A-Lyzer G3
30 mL/10K
A52973
Slide-A-Lyzer G3
70 mL/10K
A52974
Slide-A-Lyzer G3
125 mL/10K
A52975
10K (gamma irradiated)Slide-A-Lyzer G3
3 mL/10K (gamma irradiated)
A52981
Slide-A-Lyzer G3
15 mL/10K (gamma irradiated)
A52982
Slide-A-Lyzer G3
30 mL/10K (gamma irradiated)
A52983
Slide-A-Lyzer G3
70 mL/10K (gamma irradiated)
A52984
Slide-A-Lyzer G3
125 mL/10K (gamma irradiated)
A52985
20KSlide-A-Lyzer G3
3 mL/20K
A52976
Slide-A-Lyzer G3
15 mL/20K
A52977
Slide-A-Lyzer G3
30 mL/20K
A52978
Slide-A-Lyzer G3
70 mL/20K
A52979
Slide-A-Lyzer G3
125 mL/20K
A52980

Three generations of dialysis innovation

The new, third generation G3 Slide-A-Lyzer features the following design improvements over previous generations:

  • Improved ergonomics—A new re-designed frame that is lighter and significantly more compact than previous generations, making it easier to handle for loading and retrieval.
Photo showing the new Slide-A-Lyzer G3 alongside the previous Slide-A-Lyzer G2 showing its more compact, ergonomic shape
  • New cap design—The all new, quarter-turn cap is designed to be opened easily without disturbing the sample while at the same time offering secure closure with visual and haptic closure feedback. The cap opening is significantly wider offering easier sample access, an injection port is also integrated within the cap as an additional means of sample loading and retrieval.
Zoomed in photo showing the new locking cap design of the Slide-A-Lyzer G3
  • Increased collection angle—The bottom chamber angle has been increased to allow for more efficient retrieval of dialyzed sample by pipette, reducing potential sample loss.

  • Self-flotation—The Slide-A-Lyzer G3 cassette, like the G2 before it, is designed to float independently without the use of external buoys. This design has been further improved to ensure a more stable vertical orientation in the dialysis buffer, allowing for more efficient dialysis conditions.

  • Greener by design—The Slide-A-Lyzer G3 features up to a 68% reduction in plastic waste compared to previous generations while offering the same secure, high-performance dialysis. To this end, it has been included as part of our sustainable product design portfolio of products, a suite of research solutions that have been designed to reduce environmental impact.
Green Leaf Logo

See how they work:

Slide-A-Lyzer G2 Dialysis Cassettes

Slide-A-Lyzer G2 dialysis cassettes provide convenience, flexibility, and performance for sample dialysis. These second-generation (G2) Slide-A-Lyzer dialysis cassettes are free-standing, self-floating, and pipette-loadable.

Sample loading and removal are easily accomplished using a serological pipette or hypodermic needle (optional) attached to a syringe (Figure 1). The built-in air chamber provides sample buoyancy and vertical orientation of the cassette during dialysis. Slide-A-Lyzer G2 Dialysis Cassettes are available in five volume capacities and in 2K, 3.5K, 7K, 10K, and 20K MWCOs.

Choose the right Slide-A-Lyzer G2 cassette for your experiments

 
 Volume range
MWCO0.25–0.5 mL1–3 mL5–15 mL10–30 mL25–70 mL
2KSlide-A-Lyzer G2
0.5 mL/2K
87717
Slide-A-Lyzer G2
3 mL/2K
87718
Slide-A-Lyzer G2
15 mL/2K
87719
Slide-A-Lyzer G2
30 ml/2K
87720
Slide-A-Lyzer G2
70 ml/2K
87721
3.5KSlide-A-Lyzer G2
0.5 mL/3.5K
87722
Slide-A-Lyzer G2
3 mL/3.5K
87723
Slide-A-Lyzer G2
15 mL/3.5K
87724
Slide-A-Lyzer G2
30 mL/3.5K
87725
Slide-A-Lyzer G2
70 mL/3.5K
87726
7KSlide-A-Lyzer G2
0.5 mL/7K
87727
Slide-A-Lyzer G2
3 mL/7K
87728
Not availableNot availableNot available
10KSlide-A-Lyzer G2
0.5 mL/10K
87729
Slide-A-Lyzer G2
3 mL/10K
87730
Slide-A-Lyzer G2
15 mL/10K
87731
Slide-A-Lyzer G2
30 mL/10K
87732
Slide-A-Lyzer G2
70 mL/10K
87733
10K
(gamma irradiated)
Slide-A-Lyzer G2
0.5 mL/10K (gamma)
88250
Slide-A-Lyzer G2
3 mL/10K (gamma)
88251
Slide-A-Lyzer G2
15 mL/10K (gamma)
88252
Slide-A-Lyzer G2
30 mL/10K (gamma)
88253
Slide-A-Lyzer G2
70 mL/10K (gamma)
88254
20KSlide-A-Lyzer G2
0.5 mL/20K
87734
Slide-A-Lyzer G2
3 mL/20K
87735
Slide-A-Lyzer G2
15 mL/20K
87736
Slide-A-Lyzer G2
30 mL/20K
87737
Slide-A-Lyzer G2
70 mL/20K
87738

Learn more about Slide-A-Lyzer G2 dialysis cassettes

Slide-A-Lyzer G2 Dialysis Cassettes

Figure 1. Easy sample loading and recovery. Samples can be loaded into a Slide-A-Lyzer G2 Dialysis Cassette with either a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.
 

Slide-A-Lyzer original cassettes

Slide-A-Lyzer Dialysis Cassettes help facilitate the rapid and effective dialysis of sample volumes ranging from 100 μL to 30 mL. The cassette design helps maximize surface area to sample volume ratio and enables excellent sample recoveries.

In comparison to standard flat tubing, these innovative cassettes do not require knots or clips that may lead to leaking and sample loss, helping to obtain more complete sample recovery. They are available in four volume capacities and in MWCOs of 2K, 3.5K, 7K, 10K, and 20K.

Choose your Slide-A-Lyzer cassette by volume and MWCO

 Volume range
MWCO0.1–0.5 mL0.5–3 mL3–12 mL10–30 mL
2KSlide-A-Lyzer G1
0.5 mL/2K
66205
Slide-A-Lyzer G1
3 mL/2K
66203
Slide-A-Lyzer G1
12 mL/2K
66212
Slide-A-Lyzer G1
30 ml/2K
66230
3.5KSlide-A-Lyzer G1
0.5 mL/3.5K
66333
66335 (kit)
Slide-A-Lyzer G1
3 mL/3.5K
66330
66332 (kit)
Slide-A-Lyzer G1
12 mL/3.5K
66110
66107 (kit)
Slide-A-Lyzer G1
30 mL/3.5K
66130
7KSlide-A-Lyzer G1
0.5 mL/7K
66373
Slide-A-Lyzer G1
3 mL/7K
66370
66372 (kit)
Slide-A-Lyzer G1
12 mL/7K
66710
66707 (kit)
Not available
10KSlide-A-Lyzer G1
0.5 mL/10K
6638366384
66385 (kit)
Slide-A-Lyzer G1
3 mL/10K
6638066381
66382 (kit)
Slide-A-Lyzer G1
12 mL/10K
6681066811
66807 (kit)
Slide-A-Lyzer G1
30 mL/10K
66830
10K
(gamma irradiated)
Slide-A-Lyzer G1
0.5 mL/10K (gamma)
66454
Slide-A-Lyzer G1
3 mL/10K (gamma)
66455
Slide-A-Lyzer G1
12 mL/10K (gamma)
66453
Slide-A-Lyzer G1
30 mL/10K (gamma)
66456
20KSlide-A-Lyzer G1
0.5 mL/20K
66005
Slide-A-Lyzer G1
3 mL/20K
66003
Slide-A-Lyzer G1
12 mL/20K
66012
Slide-A-Lyzer G1
30 mL/20K
66030

Learn more about Slide-A-Lyzer cassettes

Table 1. Quantitative sample recovery.

Three sample volume batches of water (0.5 mL, 1.7 mL, and 3.0 mL) were loaded and recovered per the respective manufacturer’s instructions in a Slide-A-Lyzer Dialysis Cassette or conventional dialysis tubing to determine the volumes of recovery. Water volume recovered was determined gravimetrically.

Sample volume loadedSlide-A-Lyzer dialysis cassette % volume recoveryTraditional dialysis tubing % volume recovery
3.0 mL9.4792.32
1.7 mL99.3093.12
0.5 mL98.7687.51

Thermo Scientific Slide-A-Lyzer cassette procedure summary

PB2260_slide-a-lyzer_cassette_procedure1

1. Insert syringe needle through the gasket via one of the corner ports. Inject the sample, withdraw the excess air and remove the syringe.

PB2260_slide-a-lyzer_cassette_procedure2

2. Attach a float buoy and dialyze. (Buoys also serve as convenient bench-top stands for the cassettes.)

PB2260_slide-a-lyzer_cassette_procedure3

3. Insert empty syringe needle at a second corner port. Inject air to expand the cassette chamber, then withdraw the dialyzed sample.

Slide-A-Lyzer dialysis orange flasks

Thermo Scientific Slide-A-Lyzer dialysis flasks facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules in larger sample volumes up to 250 mL.

Features of Slide-A-Lyzer dialysis flasks:

  • Easy to use—Simply pipette or pour sample into flask and begin dialysis
  • Fast dialysis—Flat flask chamber with two membranes provides high surface-area to volume ratio, enabling dialysis of a 250 mL sample in eight hours to overnight
  • High recovery—Rectangular flask design maximizes recovery of entire sample volume via opening at top of flask
  • Multiple molecular-weight cut-offs—Select the membrane MWCO that best suits your sample's molecular weight
  • Color-coded frames—Easily identify membrane pore size (MWCO) based on the frame color

Slide-A-Lyzer dialysis flasks are very easy to use. Add and remove sample using a serological pipette or by directly pouring the sample through a wide screw-cap opening at the top of the flask. No knots or clips are needed to seal the units. Slide-A-Lyzer dialysis flasks are manufactured using clean room conditions and have low-protein binding regenerated cellulose membranes to enable maximum sample recovery and sample purity. The dialysis flasks are available in distinct colors, corresponding to the MWCO of the dialysis membrane: purple (20,000 daltons), orange (10,000 daltons), pink (3,500 daltons), and blue (2,000 daltons).

Slide-A-Lyzer dialysis flasks are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of an inert plastic body. The membrane and flask materials are compatible with most laboratory buffers.

With Slide-A-Lyzer dialysis flasks, typical low-molecular weight contaminant removal, buffer exchange, and desalting can be accomplished in eight hours to overnight. The rate of dialysis depends on a variety of factors, including sample volume, size and shape of the molecule being dialyzed, agitation, and temperature. Minimum dialysis time must be determined empirically for each specific system.

Choose the right Slide-A-Lyzer flask device for your experiments

MWCOMax volumeCat. No.
2K250 mL87760
3.5K230 mL87761
10K250 mL87762
20 K250 mL87763
Flotation disks for flasks

Slide-A-Lyzer flasks video

What is a dialysis cassette?

Dialysis cassettes offer a more convenient and secure alternative to traditional dialysis tubing. Traditional dialysis membrane tubing can be awkward to load and retrieve your sample, is slippery when wet and requires knots or plastic clips to secure the sample—these extra steps contribute to the possibility of lost sample. The Slide-A-Lyzer dialysis cassette offers a rigid-framed, free-standing, and self-floating vessel (G3 & G1) with a secure cap closure and large surface area regenerated cellulose membranes for simple and efficient dialysis experiments. Samples can be loaded and retrieved easily using a serological pipette or syringe.

Thermo Scientific created the original Slide-A-Lyzer G1 in 1997 as the first ready-to-use alternative to traditional tubing for dialysis experiments and has been refining the design over the past twenty years.

Three Generations of Dialysis Innovation

Cassette devices

 
 
 
Traditional tubingvs.Cassette
Slippery when wet
Flat tubing is difficult to handle when wet, increasing risk of sample loss during sample addition/removal
sample handlingEasy handling and secure sample delivery
Plastic frame and cap allow for secure sample addition/removal
Risk of sample loss
Sample can leak out if clamp becomes loose or falls off
sample recoverySample protection
Welded membrane and leak-proof cap helps minimize the risk of sample loss
Time consuming
Non-optimized design that typically requires overnight dialysis
speedFast and efficient
Innovative design with high surface/volume ratio allows for more rapid dialysis than conventional tubing

Comparison of Thermo Scientific Slide-A-Lyzer Cassettes with traditional dialysis tubing.

Need to dialyze samples smaller than 1 mL? Try Slide-A-Lyzer Mini
Need to dialyze many small samples? Try Pierce Microdialysis Plates

Related products

For Research Use Only. Not for use in diagnostic procedures.