Slide-A-Lyzer G3 dialysis cassettes and flasks consist of rigid, free-standing frames that contain large surface area regenerated cellulose membranes, and a locking cap. These devices enable easy sample loading, retrieval, and security for your dialysis experiments, and are available in volumes ranging from 1–250 mL in a large number of molecular weight cutoffs (MWCOs). Unlike standard flat tubing, these innovative devices do not require knots or clips that can lead to leakage and sample loss.
Slide-A-Lyzer G3 dialysis cassettes and flasks offer the following benefits:
Use Slide-A-Lyzer G3 dialysis cassettes and flasks in various applications, including low molecular-weight contaminant and salt removal, buffer exchange, and sample concentration.
The Slide-A-Lyzer G3 is simple to use. Load the cassette with sample, put it into the dialysis buffer, and then retrieve the sample.
Watch the video for more details.
|MWCO||1–3 mL||5–15 mL||10–30 mL||25–70 mL||65–125 mL|
|10K (E-Beam Irradiated)||Slide-A-Lyzer G3|
Important Discontinuation Notice: The Slide-A-Lyzer G2 line will be discontinued. Please consider the use of the new and improved G3 generation.
|Old Cat. No.||Slide-A-Lyzer G2 dialysis cassettes (discontinued on July 1, 2023)||New Cat. No.||Slide-A-Lyzer G1 and G3 dialysis cassettes (replacement products)|
|87717||Slide-A-Lyzer G2, 2K MWCO, 0.5 mL||66205|
|87718||Slide-A-Lyzer G2, 2K MWCO, 3 mL||A52961||Slide-A-Lyzer G3, 2K MWCO, 3 mL|
|87719||Slide-A-Lyzer G2, 2K MWCO, 15 mL||A52962||Slide-A-Lyzer G3, 2K MWCO, 15 mL|
|87720||Slide-A-Lyzer G2, 2K MWCO, 30 mL||A52963||Slide-A-Lyzer G3, 2K MWCO, 30 mL|
|87721||Slide-A-Lyzer G2, 2K MWCO, 70 mL||A52964||Slide-A-Lyzer G3, 2K MWCO, 70 mL|
|A52965||Slide-A-Lyzer G3, 2K MWCO, 125 mL|
|87722||Slide-A-Lyzer G2, 3.5K MWCO, 0.5 mL||66333||Slide-A-Lyzer G1, 3.5K MWCO, 0.5 mL|
|87723||Slide-A-Lyzer G2, 3.5K MWCO, 3 mL||A52966||Slide-A-Lyzer G3, 3.5K MWCO, 3 mL|
|87724||Slide-A-Lyzer G2, 3.5K MWCO, 15 mL||A52967||Slide-A-Lyzer G3, 3.5K MWCO, 15 mL|
|87725||Slide-A-Lyzer G2, 3.5K MWCO, 30 mL||A52968||Slide-A-Lyzer G3, 3.5K MWCO, 30 mL|
|87726||Slide-A-Lyzer G2, 3.5K MWCO, 70 mL||A52969||Slide-A-Lyzer G3, 3.5K MWCO, 70 mL|
|A52970||Slide-A-Lyzer G3, 3.5K MWCO, 125 mL|
|87727||Slide-A-Lyzer G2, 7K MWCO, 0.5 mL||66373||Slide-A-Lyzer G1, 7K MWCO, 0.5 mL|
|87728||Slide-A-Lyzer G2, 7K MWCO, 3 mL||66370||Slide-A-Lyzer G1, 7K MWCO, 3 mL|
|87729||Slide-A-Lyzer G2, 10K MWCO, 0.5 mL||66383||Slide-A-Lyzer G3, 10K MWCO, 0.5 mL|
|87730||Slide-A-Lyzer G2, 10K MWCO, 3 mL||A52971||Slide-A-Lyzer G3, 10K MWCO, 3 mL|
|87731||Slide-A-Lyzer G2, 10K MWCO, 15 mL||A52972||Slide-A-Lyzer G3, 10K MWCO, 15 mL|
|87732||Slide-A-Lyzer G2, 10K MWCO, 30 mL||A52973||Slide-A-Lyzer G3, 10K MWCO, 30 mL|
|87733||Slide-A-Lyzer G2, 10K MWCO, 70 mL||A52974||Slide-A-Lyzer G3, 10K MWCO, 70 mL|
|A52975||Slide-A-Lyzer G3, 10K MWCO, 125 mL|
|88250||Slide-A-Lyzer G2, 10K MWCO, 0.5 mL, Gamma Irradiated||66454||Slide-A-Lyzer G1, 10K MWCO, 0.5 mL, Gamma-Irradiated|
|88251||Slide-A-Lyzer G2, 10K MWCO, 3 mL, Gamma Irradiated||A52981||Slide-A-Lyzer G3, 10K MWCO, 3 mL, E-Beam Irradiated|
|88252||Slide-A-Lyzer G2, 10K MWCO, 15 mL, Gamma Irradiated||A52982||Slide-A-Lyzer G3, 10K MWCO, 15 mL, E-Beam Irradiated|
|88253||Slide-A-Lyzer G2, 10K MWCO, 30 mL, Gamma Irradiated||A52983||Slide-A-Lyzer G3, 10K MWCO, 30 mL, E-Beam Irradiated|
|88254||Slide-A-Lyzer G2, 10K MWCO, 70 mL, Gamma Irradiated||A52984||Slide-A-Lyzer G3, 10K MWCO, 70 mL, E-Beam Irradiated|
|A52985||Slide-A-Lyzer G3, 10K MWCO, 125 mL, E-Beam Irradiated|
|87734||Slide-A-Lyzer G2, 20K MWCO, 0.5 mL||66005||Slide-A-Lyzer G1, 20K MWCO, 0.5 mL|
|87735||Slide-A-Lyzer G2, 20K MWCO, 3 mL||A52976||Slide-A-Lyzer G3, 20K MWCO, 3 mL|
|87736||Slide-A-Lyzer G2, 10K MWCO, 15 mL||A52977||Slide-A-Lyzer G3, 20K MWCO, 15 mL|
|87737||Slide-A-Lyzer G2, 20K MWCO, 30 mL||A52978||Slide-A-Lyzer G3, 20K MWCO, 30 mL|
|87738||Slide-A-Lyzer G2, 20K MWCO, 70 mL||A52979||Slide-A-Lyzer G3, 20K MWCO, 70 mL|
|A52980||Slide-A-Lyzer G3, 20K MWCO, 125 mL|
Slide-A-Lyzer Dialysis Cassettes help facilitate the rapid and effective dialysis of sample volumes ranging from 100 μL to 30 mL. The cassette design helps maximize surface area to sample volume ratio and enables excellent sample recoveries.
In comparison to standard flat tubing, these innovative cassettes do not require knots or clips that may lead to leaking and sample loss, helping to obtain more complete sample recovery. They are available in four volume capacities and in MWCOs of 2K, 3.5K, 7K, 10K, and 20K.
|MWCO||0.1–0.5 mL||0.5–3 mL||3–12 mL||10–30 mL|
0.5 mL/10K (gamma)
3 mL/10K (gamma)
12 mL/10K (gamma)
30 mL/10K (gamma)
Three sample volume batches of water (0.5 mL, 1.7 mL, and 3.0 mL) were loaded and recovered per the respective manufacturer’s instructions in a Slide-A-Lyzer Dialysis Cassette or conventional dialysis tubing to determine the volumes of recovery. Water volume recovered was determined gravimetrically.
|Sample volume loaded||Slide-A-Lyzer dialysis cassette % volume recovery||Traditional dialysis tubing % volume recovery|
1. Insert syringe needle through the gasket via one of the corner ports. Inject the sample, withdraw the excess air and remove the syringe.
2. Attach a float buoy and dialyze. (Buoys also serve as convenient bench-top stands for the cassettes.)
3. Insert empty syringe needle at a second corner port. Inject air to expand the cassette chamber, then withdraw the dialyzed sample.
Slide-A-Lyzer dialysis flasks are very easy to use. Add and remove sample using a serological pipette or by directly pouring the sample through a wide screw-cap opening at the top of the flask. No knots or clips are needed to seal the units. Slide-A-Lyzer dialysis flasks are manufactured using clean room conditions and have low-protein binding regenerated cellulose membranes to enable maximum sample recovery and sample purity. The dialysis flasks are available in distinct colors, corresponding to the MWCO of the dialysis membrane: purple (20,000 daltons), orange (10,000 daltons), pink (3,500 daltons), and blue (2,000 daltons).
Slide-A-Lyzer dialysis flasks are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of an inert plastic body. The membrane and flask materials are compatible with most laboratory buffers.
With Slide-A-Lyzer dialysis flasks, typical low-molecular weight contaminant removal, buffer exchange, and desalting can be accomplished in eight hours to overnight. The rate of dialysis depends on a variety of factors, including sample volume, size and shape of the molecule being dialyzed, agitation, and temperature. Minimum dialysis time must be determined empirically for each specific system.
Dialysis cassettes offer a more convenient and secure alternative to traditional dialysis tubing. Traditional dialysis membrane tubing can be awkward to load and retrieve your sample, is slippery when wet and requires knots or plastic clips to secure the sample—these extra steps contribute to the possibility of lost sample. The Slide-A-Lyzer dialysis cassette offers a rigid-framed, free-standing, and self-floating vessel (G3 & G1) with a secure cap closure and large surface area regenerated cellulose membranes for simple and efficient dialysis experiments. Samples can be loaded and retrieved easily using a serological pipette or syringe.
Thermo Scientific created the original Slide-A-Lyzer G1 in 1997 as the first ready-to-use alternative to traditional tubing for dialysis experiments and has been refining the design over the past twenty years.
|Slippery when wet|
Flat tubing is difficult to handle when wet, increasing risk of sample loss during sample addition/removal
|sample handling||Easy handling and secure sample delivery|
Plastic frame and cap allow for secure sample addition/removal
|Risk of sample loss|
Sample can leak out if clamp becomes loose or falls off
|sample recovery||Sample protection|
Welded membrane and leak-proof cap helps minimize the risk of sample loss
Non-optimized design that typically requires overnight dialysis
|speed||Fast and efficient|
Innovative design with high surface/volume ratio allows for more rapid dialysis than conventional tubing
Comparison of Thermo Scientific Slide-A-Lyzer Cassettes with traditional dialysis tubing.
For Research Use Only. Not for use in diagnostic procedures.