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Thermo Scientific™ Slide-A-Lyzer™ MINI Dialysis Devices have a unique cup-like design and are available in 0.1, 0.5, and 2 mL capacities.
Our MINI dialysis devices come in two formats: 0.1 mL devices, which can be placed into a foam float during dialysis, and 0.5 mL and 2 mL sizes, which are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively.
The tubes serve as dialysis reservoirs for easy and self-contained dialysis, and are available in 2K, 3.5K, 7K, 10K, and 20K molecular weight cutoffs (MWCOs).
MWCO Membrane | 10–100 μL | 100–500 μL | 0.5–2 mL |
---|---|---|---|
2K | Catalog numbers 69580 69553 | Not available | Not Available |
3.5K | Catalog numbers 69550 69552 | Catalog numbers 88400 | Catalog numbers 88403 |
7K | Catalog numbers 69560 69562 | Not available | Not Available |
10K | Catalog numbers 69570 69572 | Catalog number 88401 | Catalog number 88404 |
20K | Catalog numbers 69590 69555 | Catalog number 88402 | Catalog number 88405 |
Membrane MWCO (K) | Protein/Peptide | Recovery (%) |
---|---|---|
3.5 | Insulin Chain B (3.5 kDa) | 90.13 |
10 | Cytochrome C (12.4 kDa) | 94.44 |
20 | Myoglobin (17 kDa) | 95 |
† Insulin chain B, cytochrome C and myoglobin (0.25 mg/mL) in either 50mM sodium phosphate, 75 mM NaCl at pH 7.2 or 0.2 M carbonate-bicarbonate buffer at pH 9.4 were dialyzed overnight (17 hours) at 4°C. The amount of protein in the retentate was determined using the Pierce BCA Protein Assay (Product # 23225).
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