Zeba Desalting Products

Thermo Scientific Zeba desalting products contain proprietary high-performance resins with exceptional desalting and protein-recovery characteristics. They can help process even very dilute protein samples, with high levels of protein recovery and greater than 95% retention (removal) of salts and other small molecules.

High performance—proprietary resin enables excellent protein recovery and efficient contaminant removal
Flexible—available in spin columns, filter spin plates, and cartridges for a range of needs
Fast—no fraction screening or waiting for protein to emerge by gravity flow
Economical—cost-effective products that offer great performance

Choose the right Zeba desalting device for you


	Zeba Spin Desalting Columns	Zeba 96-well Spin Plates	Zeba Chromatography Columns
Sample volume range	2 μL–4 mL	20–100 μL	15–250 μL or 100–1500 μL
Bed volume range	75 μL–10 mL	550 μL	1 mL or 5 mL
Number of devices with different volume capacities	5	1	2
Molecular weight cutoffs (MWCOs)	7K, 40K	7K, 40K	7K
Automation compatible?	No	Yes	No
FPLC compatible?	No	No	Yes
	Learn more	Order now	Order now

Compare Zeba Spin Desalting Column performance

Figure 1. Better performance with Thermo Scientific Zeba Spin Desalting Columns. Zeba Spin Desalting Columns provide excellent protein recovery while providing minimal sample dilution over a wider range of sample concentrations and volumes compared to alternative products. Zeba Spin Desalting Columns, 0.5 mL (7K MWCO)(Product # 89882), GE SpinTrap G-25 and Bio-Rad Micro BioSpin 6 spin columns were used to desalt 40, 80 and 120 μL samples of a HeLa lysate at a concentration of 0.2 and 1 mg/mL. Desalting was performed according to the manufacturers’ recommended protocols. Protein recovery was analyzed by SDS-PAGE. For each electrophoresis gel, an aliquot of starting sample equal to 1 μg of HeLa lysate was loaded in lane 1 as the load control; all other desalted samples were loaded in the gel at the same volume as the load control. Differences in intensity between lanes are a combination of protein recovery and sample dilution caused by desalting.

Table 1. Comparison of protein recovery, from the 7K MWCO and 40K MWCO, 0.5 mL Thermo Scientific Zeba Spin Desalting Columns.

			Typical % recovered from 100 μL sample
Recovery of	Concentration loaded	Size of molecule	7K Zeba Column	40K Zeba Column
Ubiquitin	0.5 mg/mL	8.7 kDa	75%	60%
α-Lactalbumin	1.0 mg/mL	14.1 kDa	85%	75%
Soybean trypsin inhibitor	0.5 mg/mL	20.1 kDa	85%	65%
Carbonic anhydrase	0.5 mg/mL	29 kDa	90%	75%
Ovalbumin	0.5 mg/mL	44 kDa	90%	85%
Bovine serum albumin	0.5 mg/mL	66 kDa	>90%	>90%
Human IgG	0.5 mg/mL	150 kDa	>90%	>90%

Table 2. Comparison of small molecule removal, for the 7K MWCO and 40K MWCO, 0.5 mL Thermo Scientific Zeba Spin Desalting Columns.

			Typical % removed from 100 μL sample
Removal of	Concentration loaded	Size of molecule	7K Zeba Column	40K Zeba Column
NaCl	1 M	58.44 Da	>99%	>99%
Dithiothreitol	0.5 M	154 Da	99%	99%
Sulfo-NHS-LC-biotin	0.27 mM	557 Da	85%	85%
Dy549 dye	0.2 mM	1026 Da	75%	90%
Bacitracin	0.5 mg/mL	1200 Da	75%	95%
Vitamin B12	0.5 mg/mL	1386 Da	85%	95%