Thermo Scientific Zeba desalting products contain proprietary high-performance resins with exceptional desalting and protein-recovery characteristics. They can help process even very dilute protein samples, with high levels of protein recovery and greater than 95% retention (removal) of salts and other small molecules.
- High performance—proprietary resin enables excellent protein recovery and efficient contaminant removal
- Flexible—available in spin columns, filter spin plates, and cartridges for a range of needs
- Fast—no fraction screening or waiting for protein to emerge by gravity flow
- Economical—cost-effective products that offer great performance
Choose the right Zeba desalting device for you
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| Zeba Spin Desalting Columns | Zeba 96-well Spin Plates | Zeba Chromatography Columns | |
| Sample volume range | 2 μL–4 mL | 20–100 μL | 15–250 μL or 100–1500 μL |
| Bed volume range | 75 μL–10 mL | 550 μL | 1 mL or 5 mL |
| Number of devices with different volume capacities | 5 | 1 | 2 |
| Molecular weight cutoffs (MWCOs) | 7K, 40K | 7K, 40K | 7K |
| Automation compatible? | No | Yes | No |
| FPLC compatible? | No | No | Yes |
| Learn more | Order now | Order now |
Compare Zeba Spin Desalting Column performance
Figure 1. Better performance with Thermo Scientific Zeba Spin Desalting Columns. Zeba Spin Desalting Columns provide excellent protein recovery while providing minimal sample dilution over a wider range of sample concentrations and volumes compared to alternative products. Zeba Spin Desalting Columns, 0.5 mL (7K MWCO)(Product # 89882), GE SpinTrap G-25 and Bio-Rad Micro BioSpin 6 spin columns were used to desalt 40, 80 and 120 μL samples of a HeLa lysate at a concentration of 0.2 and 1 mg/mL. Desalting was performed according to the manufacturers’ recommended protocols. Protein recovery was analyzed by SDS-PAGE. For each electrophoresis gel, an aliquot of starting sample equal to 1 μg of HeLa lysate was loaded in lane 1 as the load control; all other desalted samples were loaded in the gel at the same volume as the load control. Differences in intensity between lanes are a combination of protein recovery and sample dilution caused by desalting.
Table 1. Comparison of protein recovery, from the 7K MWCO and 40K MWCO, 0.5 mL Thermo Scientific Zeba Spin Desalting Columns.
| Typical % recovered from 100 μL sample |
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|---|---|---|---|---|
| Recovery of | Concentration loaded | Size of molecule | 7K Zeba Column | 40K Zeba Column |
| Ubiquitin | 0.5 mg/mL | 8.7 kDa | 75% | 60% |
| α-Lactalbumin | 1.0 mg/mL | 14.1 kDa | 85% | 75% |
| Soybean trypsin inhibitor |
0.5 mg/mL | 20.1 kDa | 85% | 65% |
| Carbonic anhydrase | 0.5 mg/mL | 29 kDa | 90% | 75% |
| Ovalbumin | 0.5 mg/mL | 44 kDa | 90% | 85% |
| Bovine serum albumin |
0.5 mg/mL | 66 kDa | >90% | >90% |
| Human IgG | 0.5 mg/mL | 150 kDa | >90% | >90% |
Table 2. Comparison of small molecule removal, for the 7K MWCO and 40K MWCO, 0.5 mL Thermo Scientific Zeba Spin Desalting Columns.
| Typical % removed from 100 μL sample |
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|---|---|---|---|---|
| Removal of | Concentration loaded | Size of molecule | 7K Zeba Column | 40K Zeba Column |
| NaCl | 1 M | 58.44 Da | >99% | >99% |
| Dithiothreitol | 0.5 M | 154 Da | 99% | 99% |
| Sulfo-NHS-LC-biotin | 0.27 mM | 557 Da | 85% | 85% |
| Dy549 dye | 0.2 mM | 1026 Da | 75% | 90% |
| Bacitracin | 0.5 mg/mL | 1200 Da | 75% | 95% |
| Vitamin B12 | 0.5 mg/mL | 1386 Da | 85% | 95% |
Biomarker quantitation assay guide
This 72-page guide provides detailed information about different tools for protein and RNA quantitation. Download this valuable technical resource that covers technologies useful for cancer and inflammation research, immunology, neurology and more. Learn more about how antibody pairs, ELISA kits, and multiplex kits for the Invitrogen Luminex platform may help advance your research.
For Research Use Only. Not for use in diagnostic procedures.
