Thermo Scientific Zeba Desalting Spin Columns are widely used in various research applications that involve the separation of proteins, and other macromolecules from soluble low molecular weight substances (e.g., salts) that may adversely impact the stability of the analytes or interfere with downstream applications. Zeba Desalting Spin Columns are also used in protein solution buffer exchange protocols for the generation of more appropriate buffers before subsequent applications such as western blotting, immunoprecipitation, activity assays, and mass spectrometry.
Zeba Desalting Spin Columns yield exceptional desalting and protein-recovery characteristics and provide consistent performance over a wide range of sample sizes and protein concentrations. High protein recovery and greater than 95% contaminant removal are achieved, even with dilute protein samples.
Zeba Desalting Spin Columns speed up the desalting process by eliminating the time needed for samples to drop out of gravity flow columns. They offer a fast and efficient protocol for the collection and concentration of clean samples in one fraction, require no chromatography system, and permit multi-sample processing.
|Zeba Spin Desalting Columns||Zeba 96-well Spin Plates||Zeba Chromatography Columns|
|Sample volume range||2 μL–4 mL||20–100 μL||15–250 μL|
|Resin Bed volume||75 μL–10 mL||550 μL||1 mL–5 mL|
|Resin MWCO*||7 kDa, 40 kDa||7 kDa, 40 kDa||7 kDa, 40 kDa|
*Molecular Weight Cut-Off (MWCO)
Gel filtration chromatography is based on molecular weight cut-off (MWCO) limits that exclude molecules based on size. Compared with dialysis, this technique has the advantages of speed, compatibility with organic and other solvents, and ability to concentrate the contaminating material into a relatively small volume, which is advantageous for toxic/radioactive substances.
Figure 1. Zeba Micro Spin Desalting Columns, 7K MWCO, recovery performance. BSA and ubiquitin samples of varying concentrations were processed using Zeba Micro Spin Desalting Columns, 7K MWCO, alongside comparable competitor products. The data show that, even at very dilute protein concentrations (250 ng), the Zeba Desalting columns outperform those of the competitors, yielding higher protein recovery for both BSA and ubiquitin.
Zeba 40K Spin Desalting Columns contain a new and enhanced high-performance size-exclusion chromatography resin that improves protein recovery and offers higher desalting and molecular weight recovery than before.
Figure 2. Superior sample recovery of low protein loads with Zeba 40K spin columns. BSA and IgG of varying concentrations were applied in 50µl aliquots to either 0.5 mL Zeba 40K, competitor C, or Competitor B spin columns. All columns were equilibrated with PBS, then centrifuged per manufacturers’ instructions for each resin. Recovered protein (%) in each flow through sample was determined by BCA assay. Flow through samples were performed on SDS-PAGE and stained with either Coomassie Blue or silver stain. Although 12.5 µg total protein load was recovered efficiently by all three resins, lower recoveries were observed with competitor resins at 1 ug and 250 ng protein loads.
Figure 3. New enhanced Zeba 40K resin is more effective than that of competitors at removing small proteins. Purified protein samples (0.5mg/mL) of varying molecular weights were applied individually in 50 ul aliquots in either 0.5 mL Zeba 40K, Competitor C, or Competitor B spin columns equilibrated in PBS. Columns were centrifuged per manufacturers’ instructions for each resin, and the recovered protein (%) in each flow-through sample was determined by BCA protein assay. As opposed to competitor columns, the Zeba 40K resin was more efficient at removing smaller proteins (evidenced by the lower small protein recovery values).
For Research Use Only. Not for use in diagnostic procedures.