Thermo Scientific Zeba desalting products contain proprietary high-performance resins with exceptional desalting and protein-recovery characteristics. They can help process even very dilute protein samples, with high levels of protein recovery and greater than 95% retention (removal) of salts and other small molecules.

  • High performance—proprietary resin enables excellent protein recovery and efficient contaminant removal
  • Flexible—available in spin columns, filter spin plates, and cartridges for a range of needs
  • Fast—no fraction screening or waiting for protein to emerge by gravity flow
  • Economical—cost-effective products that offer great performance

Choose the right Zeba desalting device for you

 Zeba Spin Desalting ColumnsZeba 96-well Spin PlatesZeba Chromatography Columns
Sample volume range2 μL–4 mL20–100 μL15–250 μL or
100–1500 μL
Bed volume range75 μL–10 mL550 μL1 mL or
5 mL
Number of devices with different volume capacities512
Molecular weight cutoffs (MWCOs)7K, 40K7K, 40K7K
Automation compatible?NoYesNo
FPLC compatible?NoNoYes
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Compare Zeba Spin Desalting column performance

Figure 1. Better performance with Thermo Scientific Zeba Spin Desalting Columns. Zeba Spin Desalting Columns provide excellent protein recovery while providing minimal sample dilution over a wider range of sample concentrations and volumes compared to alternative products. Zeba Spin Desalting Columns, 0.5 mL (7K MWCO) (Cat. No. 89882), GE SpinTrap G-25 and Bio-Rad Micro BioSpin 6 spin columns were used to desalt 40, 80 and 120 μL samples of a HeLa lysate at a concentration of 0.2 and 1 mg/mL. Desalting was performed according to the manufacturers’ recommended protocols. Protein recovery was analyzed by SDS-PAGE. For each electrophoresis gel, an aliquot of starting sample equal to 1 μg of HeLa lysate was loaded in lane 1 as the load control; all other desalted samples were loaded in the gel at the same volume as the load control. Differences in intensity between lanes are a combination of protein recovery and sample dilution caused by desalting.

Table 1. Comparison of protein recovery, from the 7K MWCO and 40K MWCO, 0.5 mL Thermo Scientific Zeba Spin Desalting Columns.

 Typical % recovered
from 100 μL sample
Recovery ofConcentration loadedSize of molecule7K Zeba Column40K Zeba Column
Ubiquitin0.5 mg/mL8.7 kDa75%60%
α-Lactalbumin1.0 mg/mL14.1 kDa85%75%
Soybean trypsin
0.5 mg/mL20.1 kDa85%65%
Carbonic anhydrase0.5 mg/mL29 kDa90%75%
Ovalbumin0.5 mg/mL44 kDa90%85%
Bovine serum
0.5 mg/mL66 kDa>90%>90%
Human IgG0.5 mg/mL150 kDa>90%>90%

Table 2. Comparison of small molecule removal, for the 7K MWCO and 40K MWCO, 0.5 mL Thermo Scientific Zeba Spin Desalting Columns.

 Typical % removed
from 100 μL sample
Removal ofConcentration loadedSize of molecule7K Zeba Column40K Zeba Column
NaCl1 M58.44 Da>99%>99%

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Biomarker quantitation assay guide

This 72-page guide provides detailed information about different tools for protein and RNA quantitation. Download this valuable technical resource that covers technologies useful for cancer and inflammation research, immunology, neurology and more. Learn more about how antibody pairs, ELISA kits, and multiplex kits for the Invitrogen Luminex platform may help advance your research.

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