zeba desalting spin columns and plates

Thermo Scientific Zeba desalting products are widely used in various research applications that involve the separation of proteins, and other macromolecules from soluble low molecular weight substances (e.g., salts) that may adversely impact the stability of the analytes or interfere with downstream applications. Zeba Desalting products are also used in protein solution buffer exchange protocols for the generation of more appropriate buffers before subsequent applications such as western blotting, immunoprecipitation, activity assays, and mass spectrometry.

Zeba Desalting products yield exceptional desalting and protein-recovery characteristics and provide consistent performance over a wide range of sample sizes and protein concentrations. These desalting products speed up the desalting process by eliminating the time needed for samples to drop out of gravity flow columns. They offer a fast and efficient protocol for the collection and concentration of clean samples in one fraction, require no chromatography system, and permit multi-sample processing.

Choose the desalting product for your experiment ›

Benefits of Zeba desalting columns, plates, and cartridges:

  • High performance—proprietary resin provides excellent protein recovery and greater than 95% contaminant removal
  • Flexible—available in spin columns, filter spin plates and cartridges for a range of needs
  • Fast— spin columns eliminate the need for fraction screening or waiting for protein to emerge by gravity flow

Choose the right desalting product for your experiment

 zeba desalting spin columnzeba desalting spin columnzeba desalting spin plate

Zeba Spin Desalting Columns

Zeba 96-well Spin Plates

Zeba Chromatography Columns

Desalting methodSpin-column allows processing within 5-8 minutesChromatography
Sample volume range2 μL–4 mL20–100 μL15–250 μL
Resin Bed volume75 μL–10 mL550 μL1 mL–5 mL
Resin MWCO*7 kDa, 40 kDa7 kDa, 40 kDa7 kDa
FPLC compatibleNoNoYes
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*Molecular Weight Cut-Off (MWCO)

Sample desalting using gel filtration chromatography

Zeba desalting products rely on gel filtration chromatography, where different molecular weight cut-off (MWCO) limits exclude molecules based on size. Compared with dialysis, this technology offers the advantages of speed, compatibility with organic and other solvents, and the ability to concentrate contaminating material down to a relatively small volume, which is useful for work with toxic/radioactive substances.

Zeba Desalting Handbook

Thermo Scientific Zeba Resin technology provides consistent performance and peace of mind. The unique, proprietary Zeba Resin enables better protein recovery, even for samples with low protein concentrations.

Download the Zeba Desalting Handbook ›

front cover of zeba desalting handbook

For Research Use Only. Not for use in diagnostic procedures.