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Thermo Scientific Zeba Spin Desalting Columns are made of low protein-binding polypropylene and are compatible with a wide range of standard laboratory instruments and consumables.
Our Zeba columns are designed for use with most swinging-bucket or fixed-angle bench- or floor-model centrifuges (please verify head clearance before use), and are available in six volume capacities and two molecular weight cut-offs (MWCOs), 7K and 40K.
The MWCO should be lower than the molecular weight of the target molecule to help ensure it is retained while contaminants are removed. Columns with 7K help provide finer resolution for smaller molecules, useful for purifying smaller proteins (e.g., cytochrome c) or peptides (e.g., insulin). Columns with 40K are better suited for large proteins (e.g., BSA), protein complexes (e.g., hemoglobin) and large enzymes (e.g,. pyruvate kinase).
Sample volume | 2–12 µL | 30–130 µL | 200–700 µL | 500–2,000 µL | 700–4,000 µL | 4,000–10,000 µL |
Resin bed volume | 75 μL | 0.5 mL | 2 mL | 5 mL | 10 mL | 20 mL |
Product type | Micro Spin Column | 0.5 mL Spin Column | 2 mL Spin Column | 5 mL Spin Column | 10 mL Spin Column | 20 mL Spin Column |
See all 7K MWCO Zeba Spin Desalting Columns, Plates, and Cartridges › |
Sample volume | 5–14 µL | 70–200 µL | 200–900 µL | 300–2,000 µL | 1,000–4,000 µL | 4,000–10,000 µL |
Resin bed volume | 75 μL | 0.5 mL | 2 mL | 5 mL | 10 mL | 20 mL |
Product type | Micro Spin Column | 0.5 mL Spin Column | 2 mL Spin Column | 5 mL Spin Column | 10 mL Spin Column | 20 mL Spin Column |
See all 40K MWCO Zeba Spin Desalting Columns, Plates, and Cartridges › |
MWCO | 7K | 7K | 7K | 40K |
Sample volume | 20–100 µL | 50–250 µL | 100–1,500 µL | 20–100 µL |
Resin bed volume | N/A | 1 mL | 5 mL | N/A |
Product type | 96-well filter plates | 1 mL Desalting Cartridges | 5 mL Desalting Cartridges | 96-well filter plates |
Additional products used to efficiently wash away contaminates and elute your purified sample:
Zeba spin desalting columns quickly and effectively remove salts and other small molecules from your sample using gel-filtration chromatography. They contain ready-to-use size exclusion resin that enables high salt retention and protein recovery. Zeba columns eliminate the need for gravity-filtration, thus speeding up the desalting process. View the video to learn how to desalt your sample using the Zeba Spin Desalting Columns.
Zeba Spin Desalting Columns are available in a variety of sizes and volume capacities compared to competitor offerings (Table 1). Zeba columns can also be used with volumes as small as 5 uL (in micro format) to as large as 10 mL
Zeba 40K Spin Desalting Columns contain a new and enhanced high-performance size-exclusion chromatography resin. These redeveloped Zeba columns demonstrate improved protein recovery for a variety of proteins in the 40 kDa and above molecular weight range (Table 2).
Recovery* | |||
---|---|---|---|
Protein | Molecular Size | 7K Zeba Column | 40K Zeba Column |
Bacitracin | 1.5 kDa | 15% | 12% |
Aprotinin | 6.5 kDa | 58% | 37% |
Ubiquitin | 8.5 kDa | 78% | 51% |
Lactalbumin | 14 kDa | 88% | 64% |
Trypsin Inhibitor | 21 kDa | 94% | 76% |
Carbonic Anhydrase | 30 kDa | 90% | 79% |
Ovalbumin | 42 kDa | 95% | 95% |
BSA | 67 kDa | 94% | 93% |
Rabbit IgG | 150 kDa | 92% | 92% |
*Recovery of 100 uL sample volume, with a loaded concentration of 0.5 mg/mL
Table 2. Protein recovery comparison using Zeba Protein Desalting Columns with 7K and 40K MWCO resin.
Zeba 7K Spin Desalting Columns contain a high-performance size-exclusion chromatography resin that allows for exceptional protein desalting and recovery. When compared to competitors, Zeba 7K desalting columns have superior protein recovery even at dilute protein concentrations (Figure 1).
Figure 1. Zeba Micro Spin Desalting Columns, 7K MWCO, recovery performance. BSA and ubiquitin samples of varying concentrations were processed using Zeba Micro Spin Desalting Columns, 7K MWCO, alongside comparable competitor products. The data show that, even at very dilute protein concentrations (250 ng), the Zeba Desalting columns outperform those of the competitors, yielding higher protein recovery for both BSA and ubiquitin.
Zeba 40K Spin Desalting Columns provide superior performance that improves protein recovery compared to competitors (Figure 2). Further, the new resin in the Zeba 40K desalting columns allows for higher desalting and is more effective at small protein removal (Figure 3).
Thermo Scientific Zeba Resin technology provides consistent performance and peace of mind. The unique, proprietary Zeba Resin enables better protein recovery, even for samples with low protein concentrations.
For Research Use Only. Not for use in diagnostic procedures.