PB2200P_protein_dialysis_desalting

Thermo Scientific ™ Zeba™ Spin Desalting Columns are made of low protein binding polypropylene, and are compatible with a wide range of standard laboratory instruments and consumables. 

Our Zeba™ columns are designed to be compatible with most swinging-bucket or fixed-angle bench- or floor-model centrifuges (please verify head clearance before use), and are available in 5 volume capacities and 2 molecular weight cut offs (MWCOs), 7K and 40K.

Choose the right Zeba™ Spin Desalting Column for you

 
Format Micro Spin Column 0.5 mL Spin Column 2 mL Spin Column 5 mL Spin Column 10 mL Spin Column
Resin bed
volume
75 μL 0.5 mL 2 mL 5 mL 10 mL
Volume capacity
(7K MWCO)
2–12 μL 30–130 μL 200–700 μL 500–2000 μL 700–4000 μL
Volume capacity
(40K MWCO)
5–14 μL 70–200 μL 200–900 μL 300–2000 μL 1000–4000 μL
7K MWCO pack sizes Catalog numbers
89877, 89878
Catalog numbers
 89882, 89883
Catalog numbers
 89889, 89890
Catalog numbers
 89891, 89892
Catalog numbers
 89893, 89894
40K MWCO pack sizes Catalog numbers
 87764, 87765
Catalog numbers
87766, 87767
Catalog numbers
87768, 87769
Catalog numbers
 87770, 87771
Catalog numbers
 87772, 87773

Compare Zeba Spin Desalting Columns

Table 1. Comparison of recommended sample volume capacity of common spin desalting products.

PB2222_zeba_common_spin_desalting

Table 2. Thermo Scientific Zeba Spin Desalting Columns provide exceptional protein recovery over a wider range of sample concentrations and volume compared to alternative products.

Desalting columns were equilibrated with a final buffer containing 25 mM Tris, 25 mM NaCl at pH7.5. Samples were dilutied in 25 mM Tris, 500 mM NaCl at pH 7.5 and then desalted into the final buffer. All samples were processed according to the manufacturers’ recommended instructions, but load volume may exceed recommended ranges (see below). There was no significant difference in salt concentration between any vendor when used within the recommended ranges. Protein concentration was determined using the Thermo Scientific™ BCA Assay (Product # 23227). Recoveries with lysate are typically slightly lower than seen with individual proteins due to the loss of small molecular weight compounds and lysis contaminants that can influence protein assay results.

  Sample Volume 7K Zeba
Spin Columns,
0.5 mL
GE
SpinTrap™ G-25
Bio-Rad Micro
Bio-Spin™
6 Columns
Sample Volume 7K Zeba
Spin Columns,
10 mL
GE
PD-10 Columns
Resin bed volume   550 µL 500 µL 700 µL   10 mL 8.3 mL 8.3 mL
Method   Spin Spin Spin   Spin Spin Drip
  % Protein Recovery % Protein Recovery
BSA 0.04 mg/mL 40 µL 70–80% <10% <10% 1.5 mL 75–90% 20–30% 40–50%***
80 µL 80–95% <10% <10% 2.5 mL 75–90% 30–40% 45–55%***
120 µL >90% <10% <20% 3.5 mL 75–90% 60–70%* 55–65%*
BSA 0.2 mg/mL 40 µL 70–80% <15% 25–35% 1.5 mL >90% 60–70% >90%***
80 µL 80–95% 30–45% 45–60% 2.5 mL >90% 70–80% 85–95%***
120 µL >90% 55–70% 65–80% 3.5 mL >90% 75–85%** 75–85%**
BSA 1 mg/mL 40 µL 80–95% 45–60% 70–80 1.5 mL >90% 80–90% >90%***
80 µL >90% 60–75% 80–90 2.5 mL >90% 80–90%* >90%***
120 µL >90% 75–90% >90% 3.5 mL >90% 80–90%* 85–95%*
HeLa Lysate 0.2 mg/mL** 40 µL 45–55% <10% <15% 1.5 mL 70–85% 55–65% >90%***
80 µL 60–70% 15–25% 30–40% 2.5 mL 70–85% 65–75% 80–95%***
120 µL 70–80% 45–55% 50–60% 3.5 mL 80–95% 70–80%* 75–85%*
HeLa Lysate 1 mg/mL** 40 µL 65–75% 45–55% 45–55% 1.5 mL 80–95% 80–90% >90%***
80 µL 75–85% 60–70% 70–80% 2.5 mL 80–95% 80–95% >90%***
120 µL 80–90% 70–80% 80–90% 3.5 mL 85–95% 80–95%* 85–95%*

* Note: Above the recommended sample volumes for the columns tested and would result in insufficient salt removal. They are included only to help demonstrate the effect of small volume on protein recovery.
** Note: Recoveries with lysate are typically slightly lower than those seen with individual proteins due to the loss of small molecular weight compounds and lysis contaminants that can influence protein assay results.
*** Note: Sample diluted during processing and recovered in 3.5 mL final volume. Protein recovery determined on total final volume.

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