Protein purification products and technologies

Find the best tools for your workflow from our broad selection of resins and formats for the purification of proteins and antibodies, or create a customized purification solution by covalent attachment of a ligand to one of our activated supports.

Our prepared and activated magnetic beads, magnetic agarose beads, standard and Superflow agarose resins, Applied Biosystem POROS resins, and other chromatography media enable protein purification at nearly any scale: assay, screening, batch, pilot, or process. In addition, we’ve developed a range of accessory products for all aspects of purification, including disposable columns and binding and elution buffers including disposable columns, binding and elution buffers, and endotoxin quantitation and removal products.

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Features of our purification resins

  • Broad product selection—prepared affinity and ion exchange supports for the purification and enrichment of proteins and antibodies, as well as activated supports for custom ligand immobilization
  • More formats—magnetic beads, 96-well filter plates, loose resins, spin columns and kits, and FPLC cartridges to facilitate screening and other small-scale experiments, as well as process-scale purification
  • High performance—resins designed to maximize protein yield and reduce background
  • Economical—pricing that is similar to or better than our leading competitors

Choose the right purification strategy
  Ion Exchange Purification Antibody Purification Fusion Protein Purification Biotin Affinity Purification Protein Immobilization
Purity level Medium to high (application dependent) High High High High
Ligand or chemistries available Strong anion exchange (SAX),
Strong cation exchange (SCX)
Protein A,
Protein G,
Protein A/G,
Protein L,
Melon gel
NeutrAvidin protein,
Monomeric avidin 
Activations for reaction to amines,
carboxylic acids,
and other groups
Base bead types available POROS resin Agarose,
Magnetic beads,
POROS resin
Superflow agarose,
Magnetic beads
Magnetic beads
Magnetic beads
Packaging options Loose resin Loose resins or beads, spin columns and kits, chromotography cartridges, 96-well spin plates Loose resins or beads, spin columns and kits, chromotography cartridges, 96-well spin plates Loose resins or beads, spin columns and kits, chromotography cartridges, 96-well spin plates Loose resins or beads, dry powder, spin columns and kits

Endotoxin contamination is a common problem during protein purification. Learn more about our endotoxin quantitation and removal products.

Featured protein purification categories

Our magnetic agarose resins offer the high binding capacity of agarose combined with the automation capabilities of magnetic beads.  We offer a variety of ligands, including Protein A/G, Ni-NTA, Glutathione, and Anti-FLAG than can help enable the high throughput purification of recombinants protein and antibodies.

Our convenient Pierce LAL Chromogenic Endotoxin Quantitation Kit  measures endotoxin levels as low as 0.1 EU/mL in 30 minutes. Our efficient endotoxin removal resin reduces endotoxin levels in biological samples up to 99% in less than 1 hour using our convenient spin column format, with protein recovery ≥ 85%.

We offer strong cation exchange (SCX) and strong anion exchange (SAX) resins and membranes. Our ion exchange (IEX) resins are rigid polymeric beads with covalent surface chemistries, which enable easier handling and packing while offering superior physical and chemical stability.

We offer multiple formats and pack sizes of Protein A, Protein G, Protein A/G, and Protein L immobilized resins that have specific affinity to immunoglobulins, allowing purification of all antibodies of a particular target class regardless of antigen specificity.

We offer a broad range affinity purification products designed for the purification of recombinant proteins—specifically cobalt and nickel ion-metal affinity chromatography (IMAC) resins for His-tagged proteins and immobilized glutathione resins for GST-tagged proteins. Small-scale resins are also available for c-myc and HA-tagged proteins.

The strong association between avidin and biotin has been successfully employed in various affinity purification schemes. We offer a variety of immobilized biotin binding supports, including avidin, streptavidin, NeutrAvidin protein, Invitrogen CaptAvidin, and monomeric avidin resins.

We offer kits in a range of formats for various immunoprecipitation (IP) and co-immunoprecipitation (co-IP) strategies, as well as products for capturing protein-nucleic acid interactions (ChIP and RIP) and solutions for enriching recombinant or modified proteins (pull-down assays).

Pipette tip-based, ready-to-use Thermo Scientific MSIA microcolumns enable affinity purification of very small quantities of target analytes from complex biological samples. Use robotics-ready MSIA microcolumns to immunoprecipitate and purify cleaner analytes with enhanced reproducibility.

Protein enrichment strategies involve the selective isolation of protein subclasses sharing a common posttranslational modification (PTM), such as phosphorylation and glycosylation, typically via affinity ligands such as ion-metal affinity chromatography (IMAC) or immobilized lectins.

We’ve developed activated supports for the immobilization of proteins and other molecules for target-specific affinity purification. The functional groups on the resins utilize a range of coupling mechanism for immobilizing amine-, sulfhydryl-, carbonyl-, and carboxyl-based targets.

For your convenience, we offer a wide range of disposable plastic columns (spin- and gravity-based) for small-scale purification needs, Thermo Scientific BupH Dry-Blend Buffer Packs, and Thermo Scientific Pierce 10X and 20X Concentrated Buffers. 

When you need high performance and high throughput on a large scale, POROS bulk chromatography resins are the industry standard for process-scale bioseparations.

Related categories

Mini- and microcentrifuges to accelerate your protein preparation protocols with RCFs up to 21,000 x g in compact, safe, easy-to-use instruments.

Designed for solid performance and consistent results, our benchtop centrifuges deliver efficient sample processing for your protein purification & isolation applications.