44894-AminoLink-Plus-230

We offer a variety of activated supports and accessories for the immobilization of proteins, antibodies, and other molecules. These resins are available separately or in convenient kits. Choose resins based on NHS-ester, AminoLink™, SulfoLink™, GlycoLink™, CarboxyLink™, and other activation technologies.

  • Product choice—a variety of coupling chemistries to meet different immobilization strategies
  • High performance—resins that maximize coupling efficiency and binding capacity
  • Flexible—available in multiple pack sizes and formats

Choose the right immobilization solution for your experiment

 
 Pierce NHS-Activated AgaroseAminoLink Coupling ResinAminoLink Plus Coupling ResinSulfoLink Coupling ResinGlycoLink Coupling ResinCarboxyLink Coupling
Target-NH2-NH2-NH2-SH-CHO-COOH
Support6% agarose (slurry or dry powder)4% agarose4% agarose, highly crosslinked6% agaroseUltralink (polyacrylamide/
azlactone copolymer)
4% agarose
Coupling capacity>25 mg/mL resin1–20 mg/mL resin20 mg/mL resin20 mg/mL resin1–10 mg/mL resin5 mg/mL resin
Coupling
time
30 min4 hr4 hr2–3.5 hr30 min4 hr
Recommended forProteinsProteins, antibodiesProteins, antibodiesProteins, peptides, antibodiesGlycoproteins, polyclonal antibodiesUnmodified peptides
AdvantagesHigh coupling efficiency and capacityHigh coupling efficiencyHigh coupling efficiencyAllows for gentle elutionCorrectly orients antibodies during couplingFlexible and gentle coupling conditions
DisadvantagesNarrow pH range (7–9)May be coupled at antigen-binding siteMay be coupled at antigen-binding siteProtein must be reduced firstNot recommended for monoclonal antibodiesNot recommended for antibodies
 26196, 26197 (dry resin),
 26200 (slurry)
20381 (10 mL), 20382 (50 mL)20501 (10 mL), 20505 (50 mL)20401 (10 mL), 20402 (50 mL), 20404 (250 mL)53149 (10 mL)20266 (25 mL)
Coupling-Efficiency-GraphThermo Scientific Pierce NHS-Activated Agarose more effectively couples dilute samples than competing products. Rabbit IgG (0.5 mg) was applied to 0.25 mL of activated agarose in PBS at pH 7.2 for 30 minutes. The protein concentration of the flow-through and washes (uncoupled IgG) was determined using the Pierce 660nm Protein Assay. The bound IgG was calculated by subtracting the amount of uncoupled IgG from the amount added. The coupling efficiencies of both dry and slurry forms of Pierce NHS-Activated Agarose were higher than NHS-activated Sepharose™ 4 Fast Flow (GE Healthcare) and Affi-Gel™ 10 Gel (Bio-Rad).
44999-002-SulfoLink-Peptide-TCEPFigure 1. TCEP effectively reduces peptide sulfhydryls (cysteine residues) to maximize covalent immobilization efficiency to SulfoLink Coupling Resin. Peptide A (0.5 mg) and human calcitonin peptide (1 mg) were incubated with (+) or without (-) 25 mM TCEP for 30 minutes and then reacted to 2 mL of SulfoLink Resin for 30 minutes using the default kit procedure. Each peptide contained a C-terminal cysteine, as well as an N-terminal fluorescent tag by which the proportion of non-coupled peptide was determined upon recovery of the reaction solution.

High-yield protein coupling

Immobilization efficiencies for various proteins on 1 mL of AminoLink Plus Resin when immobilized using the pH 7.2 coupling procedure.

ProteinProtein (mg) reactedProtein (mg) coupledCoupling efficiency (%)
Protein G4.64.083
Mouse IgG4.74.596
Rat IgG4.74.493
GAM*-IgG0.90.884
Human IgG4.84.697
Human IgM0.90.893

*GAM-IgG is goat anti-mouse IgG, a secondary antibody.

Additional activated supports and accessories are available, as well as convenient kit formats that included disposable columns, buffers, and coupling reagents. Some activated supports are also available in magnetic bead formats.