We offer a variety of products, such as magnetic beads and resins, for the purification of recombinant proteins from E. coli, yeast, insect, or mammalian cell cultures. Magnetic beads and resin have high mg-scale protein binding capacity and are available in multiple formats to accommodate a variety of needs (high-throughput screening, batch, pilot, and process purification).
|Other epitope-tagged proteins|
|Ligands available||Nickel, cobalt||Glutathione||Anti-DYKDDDDK (FLAG),|
or Anti-HA antibody
|Formats||magnetic beads, filter plates, loose resins (magnetic agarose, standard agarose and superflow agarose), spin columns and kits, FPLC cartridges||magnetic beads, filter plates, loose resins (magnetic agarose, standard agarose, and superflow agarose), spin columns and kits, FPLC cartridges||magnetic beads, loose resin (agarose, UltraLink)|
|Select products||His-tagged Protein Purification||GST-tagged Protein Purification||Epitope-tagged Protein Purification|
High-yield, high-purity, medium-scale purification of 6xHis-Tagged protein. More than 4 grams of over-expressed 6xHis-GFP were purified in 3 hours using 200 mL columns containing HisPur Ni-NTA Superflow Agarose or Qiagen Ni-NTA Superflow. One liter of lysate was loaded at a flow rate of 20 mL/min, then washed until baseline with wash buffer containing 30 mM imidazole. Bound protein was eluted with buffer containing 300 mM imidazole. Fractions containing purified 6xHis-GFP were pooled and quantitated using Pierce 660 nm Protein Assay (Cat. No. 22662). Load, flow-through, wash, and eluate fractions were separated by SDS-PAGE, stained with Imperial Protein Stain (Cat. No. 24615) and evaluated using myImageAnalysis Software (Cat. No. 62237) to determine purity.
See experiment details and additional data
Thermo Scientific Pierce Glutathione Agarose facilitates high yield and high purity GST-fusion protein purification.E. coli lysate (14.4 mg total protein) containing overexpressed GST was incubated with 50 µL GSH resin from various suppliers and purified per manufacturers' instructions. The amount of GST eluted from the resin (yield) was quantified by Thermo Scientific Coomassie Plus Protein Assay. Purity was assessed by densitometry of the stained gel lanes. M= MW marker; L=Lysate load; FT=Flow-through; E=Elution.
Use this sensitive, broad range endotoxin quantitation kit to measure endotoxin contamination between 0.01 - 1 EU/mL.
For Research Use Only. Not for use in diagnostic procedures.