We offer a variety of affinity products, such as magnetic beads and resins, for the purification of recombinant proteins from cultures of bacteria or yeast, such as E. coli or Pichia. Magnetic beads and resins are available in multiple formats to accommodate a variety of needs, from high-throughput screening, batch, pilot, and process purification. Superflow agarose resins have undergone extensive chemical characterization.

  • Broad product selection—choose affinity supports for the purification of proteins with a variety of fusion tags, including 6xHis, GST, c-myc, and HA.
  • More formats—magnetic beads, magnetic agarose, loose resin, spin columns and kits, FPLC cartridges, and 96-well filter plates enable fusion protein purification from microgram to kilogram scales.
  • High performance—resins are designed to maximize protein yield and reduce background.
  • Economical—pricing is similar to or better than other suppliers.

Choose a resin based on recombinant or epitope tag

  His-tagged (6xHis)
proteins
GST-tagged
proteins
c-Myc-tagged
proteins
HA-tagged
proteins
Ligands available Nickel, cobalt Glutathione Anti-c-Myc antibody Anti-HA antibody
Formats magnetic beads, filter plates, loose resins (magnetic agarose, standard agarose and superflow agarose), spin columns and kits, FPLC cartridges magnetic beads, filter plates, loose resins (magnetic agarose, standard agarose, and superflow agarose), spin columns and kits, FPLC cartridges magnetic beads, loose resin (agarose) magnetic beads, loose resin (agarose) 
Processing scale µg–kg µg–kg µg–mg µg–mg
Select products His-tagged Protein Purification GST-tagged Protein Purification 88842 (magnetic)
20168 (agarose)
88836 (magnetic)
26181 (agarose)

 

Example product data

High-yield, high-purity, medium-scale purification of 6xHis-Tagged protein. More than 4 grams of over-expressed 6xHis-GFP were purified in 3 hours using 200 mL columns containing HisPur Ni-NTA Superflow Agarose or Qiagen™ Ni-NTA Superflow. One liter of lysate was loaded at a flow rate of 20 mL/min, then washed until baseline with wash buffer containing 30 mM imidazole. Bound protein was eluted with buffer containing 300 mM imidazole. Fractions containing purified 6xHis-GFP were pooled and quantitated using Pierce 660 nm Protein Assay (Cat. No. 22662). Load, flow-through, wash, and eluate fractions were separated by SDS-PAGE, stained with Imperial Protein Stain (Cat. No. 24615) and evaluated using myImageAnalysis Software (Cat. No. 62237) to determine purity. See experiment details and additional data.


Thermo Scientific Pierce Glutathione Agarose facilitates high yield and high purity GST-fusion protein purification. E. coli lysate (14.4 mg total protein) containing overexpressed GST was incubated with 50 µL GSH resin from various suppliers and purified per manufacturers' instructions. The amount of GST eluted from the resin (yield) was quantified by Thermo Scientific™ Coomassie Plus Protein Assay. Purity was assessed by densitometry of the stained gel lanes. M= MW marker; L=Lysate load; FT=Flow-through; E=Elution.