Number of Transfections
5 nmol of siRNA
Plate Type (Transfection Vol.) 10 nM Final siRNA Concentration 100 nM Final siRNA Concentration
6 well (2.5 ml) 200 20
12 well (1 ml) 500 50
24 well (0.5 ml) 100 10
96 well (0.1 ml) 5000 50
  Number of Transfections
20 nmol of siRNA
 
Plate Type (Transfection Vol.) 10 nM Final siRNA Concentration 100 nM Final siRNA Concentration
6 well (2.5 ml) 800 80
12 well (1 ml) 2000 200
24 well (0.5 ml) 4000 400
96 well (0.1 ml) 20,000 2000
  Number of Transfections
40 nmol of siRNA
 
Plate Type (Transfection Vol.) 10 nM Final siRNA Concentration 100 nM Final siRNA Concentration
6 well (2.5 ml) 1600 160
12 well (1 ml) 4000 400
24 well (0.5 ml) 8000 800
96 well (0.1 ml) 40,000 4000