• Citations (9)

Description

The Silencer® Select siRNAs are classic 21-mers which incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemistry. A choice of Silencer® Select siRNAs are available—Pre-Designed or Validated. Silencer® Select Pre-Designed siRNAs are guaranteed-to-silence based on their proven design while the Silencer® Select Validated siRNAs are functionally tested to reduce target gene expression. Sets of Silencer® Select siRNA Libraries are also available in tubes or plate for high throughput screening. The Silencer® Select Positive and Negative Control RNAs should be included in every siRNA experiment.

The benefits of using Silencer® Select siRNAs include:

  • Potent—up to 100-fold more potent than currently available siRNAs and fewer off-target effects
  • Most Specific—LNA® chemical modifications reduce off-target effects by up to 90%
  • Reliable—demonstrated improvements in consistency and reliability of phenotypic results
  • Guaranteed—100% guaranteed to silence, the best guarantee in the industry

Note: Silencer® Pre-Designed & Validated siRNAs are designed using our first-generation algorithm and DO NOT include any chemical modifications. They are recommended for completing in vitro RNAi studies that were started using Silencer® siRNAs. For new studies we recommend Silencer Select siRNAs.

Approximate Turnaround Times* 
Standard purification: 4 business days 
HPLC purification: 6 business days (20 and 40 nmol); 15 business days (250 nmol) 
In Vivo Ready: 15 business days (250 nmol) 

Catalog # Pre-designed/Validated Purification Size
4390817 Pre-designed HPLC purified 20 nmol
4390818 Pre-designed HPLC purified 40 nmol
4404014 Pre-designed HPLC purified 250 nmol
4404010 Pre-designed In Vivo Ready 250 nmol
4390815 Pre-designed non-inventoried Standard purity 20 nmol
4390771 Pre-designed non-inventoried Standard purity 5 nmol
4390816 Pre-designed non-inventoried Standard purity 40 nmol
4392420 Pre-designed Standard purity 5 nmol
4392421 Pre-designed Standard purity 20 nmol
4392422 Pre-designed Standard purity 40 nmol
4427037 Pre-designed Standard purity 1 nmol
4390821 Validated HPLC purified 20 nmol
4390822 Validated HPLC purified 40 nmol
4407270 Validated HPLC purified 250 nmol
4407267 Validated In Vivo Ready 250 nmol
4390824 Validated Standard purity 5 nmol
4390825 Validated Standard purity 20 nmol
4390826 Validated Standard purity 40 nmol
4427038 Validated Standard purity 1 nmol

*Estimated time from order confirmation to ship date from Austin, TX for orders confirmed by 2 pm CST. Does not include time required for shipping (typically overnight for US and Canada deliveries, 2 days for Europe, 2-3 days elsewhere).

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Citations & References

  1. Harismendy O, Notani D, Song X, Rahim NG, Tanasa B, Heintzman N, Ren B, Fu XD, Topol EJ, Rosenfeld MG, Frazer KA (2011) 9p21 DNA variants associated with coronary artery disease impair interferon-γ signalling response. Nature 470(7333):264-8.
  2. Okada Y, Yamagata K, Hong K, Wakayama T, Zhang Y (2010) A role for the elongator complex in zygotic paternal genome demethylation. Nature 463(7280):554-8
  3. Lukas C, Savic V, Bekker-Jensen S, Doil C, Neumann B, Pedersen RS, Grøfte M, Chan KL, Hickson ID, Bartek J, Lukas J (2011) 53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of chromosomes under replication stress. Nat Cell Biol 13(3):243–53.
  4. Slevogt H, Zabel S, Opitz B, Hocke A, Eitel J, N'guessan PD, Lucka L, Riesbeck K, Zimmermann W, Zweigner J, Temmesfeld-Wollbrueck B, Suttorp N, Singer BB (2008) CEACAM1 inhibits Toll-like receptor 2-triggered antibacterial responses of human pulmonary epithelial cells. Nat Immunol 9(11):1270–8.
  5. Siu MK, Chan HY, Kong DS, Wong ES, Wong OG, Ngan HY, Tam KF, Zhang H, Li Z, Chan QK, Tsao SW, Strömblad S, Cheung AN (2010) p21-activated kinase 4 regulates ovarian cancer cell proliferation, migration, and invasion and contributes to poor prognosis in patients. Proc Natl Acad Sci U S A. 107(43):18622–7.
  6. Hardingham GE, Chawla S, Johnson CM, Bading H. (1997) Distinct functions of nuclear and cytoplasmic calcium in the control of gene expression.Nature 385(6613):260–5.
  7. Zhu XD, Zhuang Y, Ben JJ, Qian LL, Huang HP, Bai H, Sha JH, He ZG, Chen Q (2011) Caveolae-dependent endocytosis is required for class A macrophage scavenger receptor-mediated apoptosis in macrophages. J Biol Chem 286(10):8231–9.
  8. Macheiner D, Gauglhofer C, Rodgarkia-Dara C, Grusch M, Brachner A, Bichler C, Kandioler D, Sutterlüty H, Mikulits W, Schulte-Hermann R, Grasl-Kraupp B (2009) NORE1B is a putative tumor suppressor in hepatocarcinogenesis and may act via RASSF1A. Cancer Res 69(1):235-42.
  9. Vlotides G, Cooper O, Chen YH, Ren SG, Greenman Y, Melmed S (2009) Heregulin regulates prolactinoma gene expression. Cancer Res 69(10):4209-16.

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