Artistic 3D rendition of a circular plasmid

Expand your experimental possibilities beyond synthetic siRNAs using vector-based expression of target-specific shRNA and miRNA. Achieve:

  • Stable or transient target knockdown
  • Lentiviral delivery in hard-to-transfect, primary, and non-dividing cells
  • Co-expression of a fluorescent reporter for visualization

RNAi vector technologies allow you to:

  • Regulate gene inhibition with inducible RNAi expression
  • Select for a pure population of cells stably expressing an RNAi sequence
  • Control gene expression in vivo with tissue-specific promoters

Summary of Invitrogen RNAi vector technologies

Learn more
Learn more

DescriptionmicroRNA-adapted inserts for more efficient processing of the RNAi moleculeTraditional short hairpin inserts for transient or long-term RNAi
Promoter typePol IIPol III
# shRNA per vectorPolycistronic expression of miR-adapted shRNAs (chaining)Single shRNA expression per promoter
Expression trackingCo-expression of GFP allows expression to be trackedDelivery can be tracked with a separate cassette but not by shRNA expression
Vector compatibilityCompatible with most Gateway destination vectorsCompatible with BLOCK-iT destination vectors

Pre-designed and custom miR RNAi vector inserts
Lentiviral, inducible, and GFP-expressing options available


Custom shRNA vectors for any organism

Lentiviral and adenoviral options available

Highly efficient delivery of BLOCK-iT Pol II miR RNAi Expression Vectors or BLOCK-iT shRNA Vectors is necessary to achieve significant levels of knockdown. Optimizing transfection or transduction, controlling for experimental variability, and using a powerful transfection reagent vastly improves the chances for RNAi success. The use of positive and negative controls will also help in the assessment of your experiments.

Optimizing delivery conditions

  • Start with the cell-type specific protocol and transfection reagent most suitable for your experiment
  • Use a DNA plasmid that expresses a fluorescent protein, such as GFP, to monitor that the plasmid has entered the cell
  • Apply a viability indicator such as Ethidium Homodimer-1 (EthD-1) to monitor cell viability related to transfection conditions
  • Utilize Hoechst nuclei stain to measure the percent of transfected and/or dead cells to the total cell population

Technical inquires:
Our Technical Application Scientists are available to help assist you at

Ordering & Order Status inquires:
If you have questions about pre-designed RNAi orders and order status, please contact us at

If you have any questions about Custom RNAi orders and order status, please contact us at

For Research Use Only. Not for use in diagnostic procedures.