One assay surveying the entire coronavirus genome for epidemiological investigation
A major challenge for microbiologists and virologists is the prediction of patterns of evolution and emergence of disease agents. RNA viruses like the coronavirus share the biological feature of high genetic variability, which causes them to appear as clouds of mutants. Coronavirus variants also emerge through antigenic shift within animal reservoirs, such as bats and snakes.
Ion Torrent targeted next-generation sequencing (NGS) enables a streamlined research workflow for complete genome sequencing and epidemiological studies of SARS-CoV-2 (the coronavirus responsible for COVID-19). Through the use of a set of highly specific, universal coronavirus primers in combination with a high-fidelity master mix, all genomic segments are amplified and the DNA amplicons are sequenced on any Ion Torrent system to deliver highly accurate coronavirus typing in under a day.
The Ion AmpliSeq SARS-CoV-2 Research Panel consists of 2 pools with amplicons ranging from 125–275 bp in length for complete viral genome sequencing and variant detection.
Benefits of using Ion Torrent NGS include:
- Fast turnaround time—get sequencing data in as little as 2.5 hours
- Fewer operator introduced errors with automated workflow—~45 minutes of hands on time
- Accuracy of variants
- Higher success rates and increased test eligibility—analyze samples with lower viral loads
- Low input requirements with PCR-based sensitivity—amplify directly from sample
Targeted NGS workflow for Ion AmpliSeq SARS-CoV-2 Research Panel
In addition to targeted sequencing, which covers >99% of the viral genome and variants, Ion Torrent systems can be used for de novo (whole genome) sequencing of SARS-CoV-2 by NGS .
Selecting the right chip
Up to 80 samples can be multiplexed on a single Ion 540 Chip:
No. of samples
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Ion AmpliSeq Designer
Design on demand with ease and order Ion AmpliSeq research panels focused on your genes and organisms of interest
Torrent Suite Software
Perform basecalling and alignment for single samples, run plugins for secondary analysis and easily manage your NGS data
For Research Use Only. Not for use in diagnostic procedures.